The role of PACAP-mediated sperm-cumulus cell interaction in fertilization
| Project/Area Number |
22590170
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | University of Toyama |
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Principal Investigator |
TANII Ichiro 富山大学, 大学院・医学薬学研究部(医学), 教授 (40207171)
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| Co-Investigator(Kenkyū-buntansha) |
ARADATE Tadashi 富山大学, 大学院・医学薬学研究部(医学), 助教 (30303233)
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| Co-Investigator(Renkei-kenkyūsha) |
MATSUDA Kouhei 富山大学, 大学院・理工学研究部(理学), 教授 (60222303)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 発生学 / 形態形成学 / 受精 / 先体 / 卵丘細胞 / PACAP / ニューロキニンA / 精子 / 神経ペプチド / タキキニン / サブスタンスP / 精子運動能 / 先体反応 |
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| Research Abstract |
Cumulus cells have been suggested to promote fertilization although the precise mechanism remains unclear. We showed that PACAP was present in the mouse sperm acrosome and it stimulated cumulus cells to secrete fertilization-promoting factorswhich accelerate sperm penetration through the zona pellucida. We identified neurokinin A as a cumulus cell factor that enhanced both the sperm motility and acrosome reactionwhich are necessary for sperm to penetrate the zona pellucida.
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Report
(4 results)
Research Products
(14 results)
