Identification of gene networks controlling spleen organogenesis

• Project/Area Number: 22590176
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General anatomy (including Histology/Embryology)
• Research Institution: Kumamoto University
• Principal Investigator: KATSU Kenjiro 熊本大学, 発生医学研究所, 助教 (30363526)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
• Keywords: 解剖学 / 発生・分化 / 臓器形成 / 脾臓 / 臓器形式 / 臟器形成 / 脾臟

Research Abstract

The vertebrate spleen has important functions in immunity and haematopoiesis. We studiedearly spleen development using chicken embryo as a model animal. Gene expression patternsof seven transcription factors were examined by in situ hybridization. We found thatexpression of these transcription factors are temporally and spatially regulated duringspleen development. We also found that Fgf16, Fgf receptor-1, and -2 were expressed inthe spleen primordium. We proposed a model that FGF16-FGFR1/2 signaling controlsexpressions of transcription factors during spleen development.

Research Products

• [Journal Article] Multi-modal effects of BMP signaling onNodal expression in the lateral platemesoderm during left-right axis formationin the chick embryo (2013)
  • Author(s): Katsu, K., Tatsumi, N., Niki, D.,Yamamura, K-I., and Yokouchi, Y
  • Journal Title: Developmental Biology Volume: 374 Pages: 71-84
  • Peer Reviewed
• [Journal Article] Multi-modal effects of BMP signaling on Nodal expression in the lateral plate mesoderm during left-right axis formation in the chick embryo (2013)
  • Author(s): Kenjiro Katsu, Norifumi Tatsumi, Daisuke Niki, Ken-ichi Yamamura, Yuji Yokouchi
  • Journal Title: Developmental Biology Volume: 374 Issue: 1 Pages: 71-84
  • DOI: 10.1016/j.ydbio.2012.11.027
  • Peer Reviewed
• [Journal Article] BMP inhibition by DAN in Hensen's node isa critical step for the establishment ofleft-right asymmetry in the chick embryo (2012)
  • Author(s): Katsu, K., Tokumori, D., Tatsumi, N.,Suzuki, A., Yokouchi, Y
  • Journal Title: Developmental Biology Volume: 363 Pages: 15-26
  • Peer Reviewed
• [Journal Article] BMP inhibition by DAN in Hensen's node is a critical step for the establishment of left-right asymmetry in the chick embryo (2012)
  • Author(s): Kenjiro Katsu, Daisuke Tokumori, Norifumi Tatsumi, Atsushi Suzuki, Yuji Yokouchi
  • Journal Title: Developmental Biology Volume: 363 Issue: 1 Pages: 15-26
  • DOI: 10.1016/j.ydbio.2011.12.015
  • Peer Reviewed
• [Journal Article] A Secreted BMP Antagonist, Cer1, FineTunes the Spatial Organization of theUreteric Bud Tree during Mouse KidneyDevelopment (2011)
  • Author(s): Chi, L., Saarela, U., Railo, A.,Prunskaite-Hyyrylainen, R., Skovorodkin,I., Anthony, S., Katsu, K., Liu, Y., Shan,J., Salgueiro, A. M., Belo, J. A., Davies,J., Yokouchi, Y., Vainio, S. J
  • Journal Title: PLoS ONE Volume: 6
  • Peer Reviewed
• [Journal Article] A Secreted BMP Antagonist, Cerl, Fine Tunes the Spatial Organization of the Ureteric Bud Tree during Mouse Kidney Development (2011)
  • Author(s): Chi, L., Saarela, U., Railo, A., Prunskaite-Hyyrylainen, R., Skovorodkin, I., Anthony, S., Katsu, K., Liu, Y., Shan, J., Salgueiro, AM, Belo, JA, Davies, J., Yokouchi, Y., Vainio, SJ
  • Journal Title: PLOS ONE Volume: 6 Issue: 11 Pages: e27676-e27676
  • DOI: 10.1371/journal.pone.0027676
  • Peer Reviewed
• [Presentation] 脾臓形成を制御する遺伝子ネットワークの同定とその作用機序 (2012)
  • Author(s): 勝 賢二郎、仁木大輔、辰巳徳史、横内裕二
  • Organizer: 第117回 日本解剖学会総会・全国学術集会
  • Place of Presentation: 山梨大学甲府キャンパス(山梨県)
  • Year and Date: 2012-03-26
• [Presentation] 脾臓形成を制御する遺伝子ネットワークの同定とその作用機序 (2012)
  • Author(s): 勝賢二郎, 他
  • Organizer: 第117回日本解剖学会総会・全国学術集会
  • Place of Presentation: 山梨大学甲府キャンパス(山梨県)
  • Year and Date: 2012-03-26
• [Presentation] Expression patterns of transcription factors in the chick developing spleen (2011)
  • Author(s): Katsu,K. and Yokouchi,Y
  • Organizer: 第88回日本生理学会大会・第116回日本解剖学会総会・全国学術集会 合同大会
  • Place of Presentation: パシフィコ横浜(神奈川県)