Analysis of the mechanism of blood cell- and bone marrow-derivedcell-induced separation of the blood and lymphatic vasculature

• Project/Area Number: 22590187
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General anatomy (including Histology/Embryology)
• Research Institution: The University of Tokyo
• Principal Investigator: ICHISE Taeko 東京大学, 医科学研究所, 助教 (00396863)
• Co-Investigator(Kenkyū-buntansha): ICHISE Hirotake 東京大学, 医科学研究所, 助教 (10313090)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: リンパ管 / PLC-gamma2 / VEGFR-3 / Cre/loxP / マウス / 巨核球 / 血小板

Research Abstract

We have previously demonstrated that PLC?2 plays an essential role in initiating and maintaining the separation of the blood and lymphatic vasculature. To further investigate the PLC?2-mediated mechanism by which lymphatic vessels are separated from blood vessels, we generated genetically engineered mice expressing PLC?2 in cell type-specific, Cre/loxP-dependent manners. Using the mice, we obtained results suggesting that megakaryocytes might play a central role in the vascular separation.

Research Products

• [Journal Article] Ras/MAPK Signaling Modulates VEGFR-3 Expression through Ets-Mediated p300 Recruitment and Histone Acetylation on the Vegfr3 Gene in Lymphatic Endothelial Cells. (2012)
  • Author(s): Ichise, T., Yoshida, N., Ichise, H.
  • Journal Title: PLoS One. Volume: 7 Issue: 12 Pages: e51639-e51639
  • DOI: 10.1371/journal.pone.0051639
  • Peer Reviewed
• [Journal Article] Y.Platelet activation receptor CLEC-2 regulates blood/lymphatic vessel separation by inhibiting proliferation, migration, and tube formation of lymphatic endothelial cells. (2012)
  • Author(s): Osada, M., Inoue, O., Ding, G., Shirai, T., Ichise, H., Hirayama, K., Takano, K., Yatomi, Y., Hirashima, M., Fujii, H., Suzuki-Inoue, K., Ozaki
  • Journal Title: The Journal of biological chemistry. Volume: 287 Issue: 26 Pages: 22241-22252
  • DOI: 10.1074/jbc.m111.329987
  • Peer Reviewed
• [Journal Article] N., Miki, H.Nucleoredoxin sustains Wnt/β-catenin signaling by retaining a pool of inactive Dishevelled protein. (2010)
  • Author(s): Funato, Y., Terabayashi, T., Sakamoto, R., Okuzaki, D., Ichise, H., Nojima, H.,Yoshida
  • Journal Title: Current biology. Volume: 20 Issue: 21 Pages: 1945-1952
  • DOI: 10.1016/j.cub.2010.09.065
• [Journal Article] Nucleoredoxin negatively regulates Toll-like receptor 4 signaling via recruitment of flightless-I to myeloid differentiation primary response gene (88). (2010)
  • Author(s): Hayashi, T., Funato, Y., Terabayashi, T., Morinaka, A., Sakamoto, R., Ichise, H., Fukuda, H., Yoshida, N., Miki, H.
  • Journal Title: The Journal of biological chemistry. Volume: 285 Issue: 24 Pages: 18586-18593
  • DOI: 10.1074/jbc.m110.106468
• [Journal Article] リンパ管新生におけるRasの役割 (2010)
  • Author(s): 市瀬広武
  • Journal Title: リンパ学 Volume: 33 Pages: 102-106
  • URL: http://mol.medicalonline.jp/archive/search-jo=ed6lymph&ye=2010&vo=33&issue=2
• [Presentation] A Modulatory Role of Ras in VEGFR-3Signalling during Normal and PathologicalLymphangiogenesis (2011)
  • Author(s): Hirotake Ichise
  • Organizer: 第32回 日本炎症・再生医学会シンポジウム "Tumor Microenvironment and Inflammation"
  • Place of Presentation: 国立京都国際会館,京都
  • Year and Date: 2011-06-02
• [Presentation] リンパ管新生におけるRasの役割 (2010)
  • Author(s): 市瀬広武
  • Organizer: 第34回日本リンパ学会総会
  • Place of Presentation: 東京大学山上会館,東京
  • Year and Date: 2010-06-26
• [Presentation] リンパ管新生におけるRasの役割 (2010)
  • Author(s): 市瀬広武
  • Organizer: 第34回日本リンパ学会総会
  • Place of Presentation: 東京大学山上会館
  • Year and Date: 2010-06-26
• [Presentation] Role of Ras in lymphangiogenesis (2010)
  • Author(s): Hirotake Ichise
  • Organizer: Gordon Research Conference 2010 "Molecular Mechanisms in Lymphatic Function & Disease"
  • Place of Presentation: Lucca (Barga), Italy
  • Year and Date: 2010-06-15
• [Presentation] Role of Ras in lymphangiogenesis Gordon Research Conference 2010 (2010)
  • Author(s): Hirotake Ichise
  • Organizer: Molecular Mechanisms in Lymphatic Function & DiseaseJune 15
  • Place of Presentation: Lucca (Barga), Italy
• [Presentation] Modulatory Role of Ras in VEGFR-3 Signalling during Normal and Pathological Lymphangiogenesis
  • Author(s): Hirotake Ichise
  • Organizer: 第３２回 日本炎症・再生医学会
  • Place of Presentation: 国立京都国際会館（京都）
  • Invited
• [Book] よくわかるリンパ浮腫のすべて (2011)
  • Author(s): 市瀬広武,光嶋 勲
  • Total Pages: 213
  • Publisher: 永井書店
• [Book] よくわかるリンパ浮腫のすべて （分担執筆） (2011)
  • Author(s): 市瀬 広武 （光嶋 勲 編著）
  • Publisher: 永井書店