Molecular Cell Biology of a Novel SignalingSystem in Neurons

• Project/Area Number: 22590189
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General anatomy (including Histology/Embryology)
• Research Institution: University of Yamanashi
• Principal Investigator: TAKEDA Sen 山梨大学, 医学工学総合研究部, 教授 (20272429)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 神経細胞 / 一次繊毛 / グリア細胞 / シグナル伝達系 / ヘッジホッグ / 神経膠細胞 / 情報伝達 / 細胞内輸送 / G蛋白質共役型受容体(GPCR) / 一次線毛

Research Abstract

I analyzed various functions of primary cilia in glial cells, in addition to the elucidation of neuronal primary cilia. Thesenovel findings on the molecular mechanisms underlying the signaling processes throughprimary cilia may pave the way into analysis in terms of neuron-glia interaction. Furthermore, stimulation of neuronal primary cilia with agonist to Hedgehog receptors provoked intracellular oscillation that may lead to dedifferentiation of the cells. I will further focus on this intriguing phenomenon in future project.

Research Products

• [Journal Article] Biomolecule analysis and cancer diagnostics by negative mode probe electrospray ionization (2013)
  • Author(s): Mandal, M.K., Subhrakanti, S., Yoshimura, K., Shida, Y., Takeda, S. Nonami, H. and Hiraoka
  • Journal Title: Analyst
  • Peer Reviewed
• [Journal Article] Characterization of ciliary targeting sequence of rat melanin-concentrating hormone receptor 1 (2013)
  • Author(s): Nagata A, Hamamoto A, Horikawa M, Yoshimura K, Takeda S, Saito Y
  • Journal Title: General and Comparative Endocrinology Volume: 188 Issue: 1 Pages: 159
  • DOI: 10.1016/j.ygcen.2013.02.020
  • Peer Reviewed
• [Journal Article] Hedgehog signaling regulates the myelination of peripheral nerve axons through the primary cilia (2012)
  • Author(s): Yoshimura, K. and Takeda, S.
  • Journal Title: Differentiation Volume: 83
  • Peer Reviewed
• [Journal Article] Categories of cilia: Structure and function of vertebrate cilia, towards new taxonomy. (2012)
  • Author(s): Takeda, S. and Narita, K
  • Journal Title: Differentiation Volume: 83
  • Peer Reviewed
• [Journal Article] Innovations in analytical oncology - Status quoof mass spectrometry-based diagnostics for malignant tumor- (2012)
  • Author(s): Takeda, S., Yoshimura. K. and Hiraoka, K.
  • Journal Title: Journal of Analytical Oncology Volume: 1 Pages: 74-80
  • Peer Reviewed
• [Journal Article] Proteomic analysis of multiple primary cilia reveals a novel mode of ciliary development in mammals (2012)
  • Author(s): Narita, K., Kozuka-Hata, H., Nonami, Y., Ao-Kondo, H., Suzuki, T., Nakamura, H., Yamakawa, K., Oyama, M., Inoue, T. and Takeda, S.
  • Journal Title: Biology Open Volume: 1 Pages: 815-25
  • Peer Reviewed
• [Journal Article] Analysis of renal cell carcinoma as a first step for mass spectrometry-based diagnostics (2012)
  • Author(s): Yoshimura, K., Chen, L.C., Nakazawa, T., Mandal, M.K., Yu, Z., Uchiyama, T., Hori, H., Tanabe, K., Kubota, T., Fujii, H., Katoh, R., Hiraoka, K. and Takeda, S.
  • Journal Title: Journal of the American Society for Mass Spectrometry Volume: 23 Pages: 1741-49
  • Peer Reviewed
• [Journal Article] Application of probe electrospray ionization mass spectrometry (PESI-MS) to clinical diagnosis. Solvent effects on lipid analysis (2012)
  • Author(s): Mandal,M.K., Yoshimura, K., Chen, L.C., Yu, Z., Nakazawa, T., Katoh, R., Fujii, H., Takeda, S*. and Hiraoka*. K
  • Journal Title: Journal of the American Society for Mass Spectrometry Volume: 23
  • Peer Reviewed
• [Journal Article] Anatomy of the levator claviculae muscle, with an overview and its history (2012)
  • Author(s): Odate, T., Kawai, M., Iio, K., Funayama, S., Futamata, H. and Takeda, S.
  • Journal Title: Anatomical Science International Volume: 87 Pages: 203-211
  • Peer Reviewed
• [Journal Article] Solid probe assisted nonoelectrospray ionization mass spectrometry for biological tissue diagnostics (2012)
  • Author(s): Mandal,M.K., Yoshimura, K., Saha, S., Ninomiya, S., Rahman, Md. O., Yu, Z., Chen, L.C., Shida, Y., Takeda, S. Nonami, H. and Hiraoka.
  • Journal Title: Analyst Volume: 137 Pages: 4658-61
  • Peer Reviewed
• [Journal Article] Hedgehog signaling regulates the myelination of peripheral nerve axons through the primary cilia. (2012)
  • Author(s): Kentaro Yoshimura
  • Journal Title: Differentiation Volume: 83
  • Peer Reviewed
• [Journal Article] Structure and function of vertebrate cilia, towards new taxonomy. (2012)
  • Author(s): Sen Takeda
  • Journal Title: Differentiation Volume: 83
  • Peer Reviewed
• [Journal Article] Proteomic analysis of multiple primary cilia reveals a novel mode of ciliary development in mammals (2012)
  • Author(s): Keishi Narita
  • Journal Title: Biology Open Volume: 1 Issue: 8 Pages: 815
  • DOI: 10.1242/bio.20121081
  • Peer Reviewed
• [Journal Article] 神経系細胞の一次繊毛にみられるセンサー機能 X. ニューロサイエンスの最前線 (2012)
  • Author(s): 竹田扇
  • Journal Title: Clinical Neuroscience Volume: 30 Pages: 465-468
• [Journal Article] 神経系細胞の一次繊毛にみられるセンサー機能 (2012)
  • Author(s): 竹田扇、成田啓之
  • Journal Title: Clinical Neuroscience Volume: 30 Pages: 465-8
• [Journal Article] Signaling via primary cilium affects glial cell survival under the stressed environment (2011)
  • Author(s): Yoshimura, K., Kawate, T. and Takeda, S.
  • Journal Title: Signaling via primary cilium affects glial cell survival under the stressed environment Volume: 59 Pages: 333-344
  • Peer Reviewed
• [Journal Article] Differential neuroprotective activity of two different grape seed extracts (2011)
  • Author(s): Narita, K., Hisamoato N., Okuda,T. and Takeda, S.
  • Journal Title: PLoS One Volume: 6
  • Peer Reviewed
• [Journal Article] Real time analysis of living animals by electrospray ionization mass spectrometry (2011)
  • Author(s): Yoshimura, K., Chen, LL., Yu, Z., Hiraoka, K. and Takeda, S.
  • Journal Title: Analytical Biochemistry Volume: 417 Pages: 195-201
  • Peer Reviewed
• [Journal Article] Structure and function of vertebrate cilia, towards a new taxonomy (2011)
  • Author(s): Takeda, S., Narita, K.
  • Journal Title: Differentiation Volume: 83 Issue: 2 Pages: S4-S11
  • DOI: 10.1016/j.diff.2011.11.002
  • Peer Reviewed
• [Journal Article] Hedgehog signaling regulates myelination in the peripheral nervous system through primary cilia (2011)
  • Author(s): Yoshimura, K., Takeda, S.
  • Journal Title: Differentiation Volume: 83 Issue: 2 Pages: S78-S85
  • DOI: 10.1016/j.diff.2011.10.006
  • Peer Reviewed
• [Journal Article] Signaling via Primary Cilium Affects Glial Cell Survival Under the Stressed Environment. (2011)
  • Author(s): Yoshimura, et al.
  • Journal Title: Glia Volume: 59 Pages: 333-344
  • Peer Reviewed
• [Journal Article] Differential Neuroprotective Activity of two Different Grape Seed Extracts. (2011)
  • Author(s): Narita, et al.
  • Journal Title: PLoS One Volume: 6
  • Peer Reviewed
• [Journal Article] Multiple primary cilia modulate the fluid transcytosis in choroid plexus epithelium (2010)
  • Author(s): Narita, K.,Kawate,T., Kakinuma, N. and Takeda, S.
  • Journal Title: Traffic Volume: 11 Pages: 287-301
  • Peer Reviewed
• [Journal Article] Direct analysis of lipids in mouse brain using electrospray droplet impact/SIMS. (2010)
  • Author(s): Asakawa, D., Yoshimura, T, Takeda, S., and Hiraoka, K.
  • Journal Title: Journal of Mass Spectrometry Volume: 45 Pages: 437-443
  • Peer Reviewed
• [Journal Article] Multiple Primary Cilia Modulate the Fluid Transcytosis in Choroid Plexus Epithelium. (2010)
  • Author(s): Narita, et. al.
  • Journal Title: Traffic Volume: 11 Pages: 287-301
  • Peer Reviewed
• [Journal Article] Characterization of ciliary targeting sequence of rat melanin-concentrating hormone receptor 1.
  • Author(s): Nagata, S., Hamamoto, A., Horikawa, M., Yoshimura, K., Takeda, S. and Saito, Y
  • Journal Title: General and Comparative Endocrinology Volume: 2013
  • Peer Reviewed
• [Presentation] 一次繊毛を介した末梢神経髄鞘形成の分子メカニズム (2013)
  • Author(s): 竹田 扇
  • Organizer: 第118回日本解剖学会全国学術集会
  • Place of Presentation: 香川
  • Year and Date: 2013-03-30
• [Presentation] 新しいイオン化法と機械学習を応用したがん診断支援装置の開発 (2013)
  • Author(s): 竹田 扇
  • Organizer: がんプロフェッショナル養成基盤推進プラン 大学間連携トランスレーショナル研究キックオフシンポジウム
  • Place of Presentation: 東京
  • Year and Date: 2013-03-09
• [Presentation] New approaches to the cancer diagnosis - Combination of probe electrospray ionization and machine learning - (2012)
  • Author(s): Sen TAKEDA
  • Organizer: University of Yamanashi International Symposium. UYIS2012.
  • Place of Presentation: Kofu
  • Year and Date: 2012-12-03
• [Presentation] 一次繊毛を介した 3 型ソマトスタチン受容体によるカルシウム動態の調節 (2012)
  • Author(s): 吉村健太郎、三澤透、濱本明恵、斎藤祐見子、竹田 扇
  • Organizer: 第35回日本神経科学大会
  • Place of Presentation: 名古屋
  • Year and Date: 2012-09-19
• [Presentation] What's New in Cancer Diagnostics? - An Upcoming Innovative Approach Based on Mass Spectrometry and Logistics Regression - (2012)
  • Author(s): Sen TAKEDA
  • Organizer: 14th International Congress of Histochemistry and Cytochemistry.Symposium.
  • Place of Presentation: Kyoto
  • Year and Date: 2012-08-28
• [Presentation] 関東 4 大学コンソーシアムの取組について (2012)
  • Author(s): 竹田 扇
  • Organizer: 第117回日本解剖学会全国学術集会
  • Place of Presentation: 甲府
  • Year and Date: 2012-03-26
• [Presentation] Hedgehog signaling regulates the myelination of peripheral nerve axons through the primary cilia (2011)
  • Author(s): 竹田扇
  • Organizer: 51^<st> Annual Meeting of American Society for Cell Biology
  • Place of Presentation: Colorado Convention Center (Denver, CO)
  • Year and Date: 2011-12-06
• [Presentation] Hedgehog signaling regulates the myelination of peripheral nerve axons through the primary cilia (2011)
  • Author(s): Sen Takeda and Kentaro Yoshimura
  • Organizer: 51st Annual Meeting of ASCB
  • Place of Presentation: Denver, USA
  • Year and Date: 2011-12-05
• [Presentation] シュワン細胞の一次繊毛によるヘッジホックシグナルの受容はミエリン形成を促進する (2011)
  • Author(s): 竹田扇
  • Organizer: 第34回日本神経科学大会
  • Place of Presentation: パシフィコ横浜(横浜)
  • Year and Date: 2011-09-17
• [Presentation] シュワン細胞の一次繊毛によるヘッジホッグシグナルの受容はミエリン形成を促進する (2011)
  • Author(s): 吉村健太郎、竹田 扇
  • Organizer: 第34回日本神経科学大会
  • Place of Presentation: 横浜
  • Year and Date: 2011-09-16
• [Presentation] 質量分析法の術中迅速診断への応用 (2011)
  • Author(s): 竹田 扇
  • Organizer: 第38回 BMS カンファレンス
  • Place of Presentation: 箱根
  • Year and Date: 2011-07-12
• [Presentation] 質量分析法の術中迅速診断への応用 (2011)
  • Author(s): 竹田扇
  • Organizer: 第38回BMSカンファレンス
  • Place of Presentation: 箱根高原ホテル(神奈川)(招待講演)
  • Year and Date: 2011-07-12
• [Presentation] 神経系細胞の感覚受容モジュールとしての繊毛の役割 (2011)
  • Author(s): 竹田扇
  • Organizer: 第116回日本解剖学会全国学術集会
  • Place of Presentation: 横浜市(紙面開催)
  • Year and Date: 2011-03-28
• [Presentation] 神経系細胞の感覚受容モジュールとしての繊毛の役割 (2011)
  • Author(s): 竹田 扇
  • Organizer: 第116回日本解剖学会全国学術集会
  • Place of Presentation: 横浜
  • Year and Date: 2011-03-27
• [Presentation] 肉眼解剖学実習を通じた医師・看護師双方向性教育の意義 - その効果と今後の指針 - (2011)
  • Author(s): 竹田 扇
  • Organizer: 第116回日本解剖学会全国学術集会
  • Place of Presentation: 横浜
  • Year and Date: 2011-03-27
• [Presentation] Multiple primary cilia modulate the fluid transcytosis in choroid plexus epithelium (2010)
  • Author(s): S. Takeda and K. Narita
  • Organizer: 50th Annual Meeting of ASCB, Pennsylvania Convention Center
  • Place of Presentation: Pennsylvania, USA
  • Year and Date: 2010-12-14
• [Presentation] Multiple primary cilia modulate the fluid transcytosis in choroid plexus epithelium. (2010)
  • Author(s): 竹田扇
  • Organizer: 50th Annual Meeting of the American Society for Cell Biology
  • Place of Presentation: Philadelphia(米国)
  • Year and Date: 2010-12-13
• [Presentation] Integrated science としてのバイオエシックス - 本邦のバイオエシックス草創期における今堀和友の役割 - (2010)
  • Author(s): 竹田 扇
  • Organizer: 日本生命倫理学会第22回年次大会
  • Place of Presentation: 藤田保健衛生大学 (愛知県)
  • Year and Date: 2010-11-20
• [Presentation] 神経系繊毛の多様な機能と病態 (2010)
  • Author(s): 竹田 扇
  • Organizer: 第一回繊毛研究会
  • Place of Presentation: 自然科学研究機構生理学研究所(愛知県)
  • Year and Date: 2010-11-13
• [Presentation] 生きた動物個体への質量分析法の応用と今後の課題 (2010)
  • Author(s): 竹田 扇
  • Organizer: 第58回質量分析総合討論会
  • Place of Presentation: エオポカルつくば (茨城県)
  • Year and Date: 2010-06-18
• [Presentation] Primary cilia as a novel functional module of the nervous system. (2010)
  • Author(s): Sen TAKEDA
  • Organizer: The 87th Annual Meeting of the Physiological Society of Japan
  • Place of Presentation: Malios Morioka (Iwate)
  • Year and Date: 2010-05-20
• [Presentation] Primary cilia as anovel functional module of the nervous system. (2010)
  • Author(s): 竹田扇
  • Organizer: The87^<th> Annual Meeting of the Physiological Society of Japan.
  • Place of Presentation: 盛岡
  • Year and Date: 2010-05-20
• [Presentation] 一次繊毛を介した末梢神経髄鞘形成の分子メカニズム
  • Author(s): 竹田扇
  • Organizer: 第118回日本解剖学会全国学術集会.
  • Place of Presentation: サンポート高松 (香川県)
  • Invited
• [Presentation] 16. 一次繊毛を介した3型ソマトスタチン受容体によるカルシウム動態の調節
  • Author(s): 竹田扇
  • Organizer: 第35回日本神経科学大会
  • Place of Presentation: 名古屋国際会議場 (愛知県)
• [Presentation] Multiple functions of glial primary cilia. In: Emerging roles of primary cilia in neuronal function
  • Author(s): Sen Takeda
  • Organizer: Neuro2013
  • Place of Presentation: 京都国際会議場 (京都府)
  • Invited
• [Book] カラーアトラス機能組織学 (2013)
  • Author(s): 竹田 扇
  • Publisher: エルゼビア・ジャパン/医歯薬出版
• [Book] Special Issue on Cilia (2012)
  • Author(s): Takeda, S. and Woodland, H.
  • Total Pages: 114
  • Publisher: Differentiation.2012. Elsevier
• [Book] Special Issue on Cilia (2012)
  • Author(s): Sen Takeda
  • Total Pages: 113
  • Publisher: Elsevier
• [Book] 神経細胞の細胞骨格 精神科臨床リュミエール 第16 巻『精神科医のための脳科学:これだけは知っておきたい基礎知識』 (2010)
  • Author(s): 竹田 扇
  • Publisher: 中山書店
• [Book] 精神科臨床リュミエール第16巻『精神科医のための脳科学:これだけは知っておきたい基礎知識』「神経細胞の細胞骨格」 (2010)
  • Author(s): 竹田扇(分担執筆)
  • Publisher: 中山書店
• [Remarks]
  • URL: http://www.cellbiology.jp
• [Remarks] 山梨大学大学院医学工学総合研究部 解剖学講座細胞生物学教室 HP
  • URL: http://www.cellbiology.jp
• [Remarks]
  • URL: http://www.cellbiology.jp/
• [Remarks]
  • URL: http://www.cellbiology.jp/
• [Patent(Industrial Property Rights)] 質量分析装置、及び該装置を用いた癌診断装置 (2012)
  • Inventor(s): 出水 秀明、谷畑 博司、糸井 弘人、田邉 國士、竹田扇、吉村健太郎、平岡賢三、Lee Chuin Chen、堀裕和
  • Industrial Property Rights Holder: 株式会社島津製作所、田邉 國士、竹田扇、国立大学法人山梨大学
  • Filing Date: 2012-08-27