Analysis of the transcriptional regulation of gametogenesis involving SOHLH proteins

• Project/Area Number: 22590190
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General anatomy (including Histology/Embryology)
• Research Institution: Osaka University
• Principal Investigator: MIYAZAKI Junichi 大阪大学, 大学院・医学系研究科, 教授 (10200156)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 細胞分化 / 組織形成 / 生殖細胞 / 発生・分化 / 転写因子 / 発現制御 / 遺伝学 / 遺伝子 / 解剖学

Research Abstract

bHLH proteins, SOHLH2 and SOHLH1, are expressed specifically in spermatogonia and oocytes, and are required for the early spermatogonial and oocyte differentiation. We demonstrated that SOHLH2 and SOHLH1 could form a heterodimerand that the SOHLH2/SOHLH1 heterodimer could up-regulate the Sohlh1 gene through its E-boxes. Subsequent studies suggested that SOHLH2/SOHLH1/SP1 ternary complex autonomously and cooperatively regulates the Sohlh1 gene transcription during early spermatogenesis and oogenesis.

Research Products

• [Journal Article] Embryonic stem cells maintain an undifferentiated state on dendrimer-immobilized surface with D-glucose display (2011)
  • Author(s): Shohreh Mashayekhan, Mee-Hae Kim, Masahiro Kino-oka, Jun-ichi Miyazaki, Masahito Taya
  • Journal Title: Polymers Volume: Vol.3, No.4 Issue: 4 Pages: 2078-2087
  • DOI: 10.3390/polym3042078
  • Peer Reviewed
• [Journal Article] Maternal-effect gene Ces5/Ooep/Moep19/Floped is essential for oocyte cytoplasmic lattice formation and embryonic development at the maternal-zygotic stage transition (2010)
  • Author(s): Tashiro F, Kanai-Azuma M, Miyazaki S, Kato M, Tanaka T, Toyoda S, Yamato E, Kawakami H, Miyazaki T and Miyazaki J
  • Journal Title: Genes to Cells Volume: 15 Pages: 813-828
  • Peer Reviewed
• [Journal Article] Maternal-effect gene Ces5/Ooep/Moep19/Floped is essential for oocyte cytoplasmic lattice formation and embryonic developmentat the maternal-zygotic stage transition. (2010)
  • Author(s): Tashiro F, Kanai-Azuma M, Miyazaki S, Kato M, Tanaka T, Toyoda S, Yamato E, Kawakami H, Miyazaki T, Miyazaki J
  • Journal Title: Genes to Cells Volume: 15 Pages: 813-828
  • Peer Reviewed
• [Journal Article] Nuclear hormone receptor RXR participates in the glucose-stimulated insulin secretion of pancreatic β cells in mice (2010)
  • Author(s): Miyazaki S, Taniguchi H, Moritoh Y, Tashiro F, Yamamoto T, Yamato E, Ikegami H, Ozato K, Miyazaki J
  • Journal Title: Diabetes Volume: 59 Pages: 2854-2861
  • Peer Reviewed
• [Presentation] マウス ES 細胞の未分化状態維持に関与する Ces1 遺伝子の解析. (2012)
  • Author(s): 田代 文、 宮崎竜志、宮崎早月、松浦 巧、倭 英司、 宮崎純一
  • Organizer: 分子生物学会年会
  • Place of Presentation: マリンメッセ福岡
  • Year and Date: 2012-12-12
• [Presentation] マウスES細胞の未分化状態維持に関与するCes1遺伝子の解析 (2012)
  • Author(s): 田代 文
  • Organizer: 日本分子生物学会
  • Place of Presentation: マリンメッセ福岡
• [Presentation] 精巣におけるレトロトランスポゾン発現抑制に関与するマウス Cue110 遺伝子の機能解析. (2011)
  • Author(s): 能村卓慈、宮川 さとみ、宮崎純一
  • Organizer: 分子生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2011-12-13
• [Presentation] 精巣におけるレトロトランスポゾン発現抑制に関与するマウスCue110遺伝子の機能解析 (2011)
  • Author(s): 能村卓慈、宮川さとみ、宮崎純一
  • Organizer: 分子生物学会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2011-12-13
• [Presentation] 精子形成・卵形成初期における SOHLH2/SOHLH1/SP1 複合体による Sohlh1 遺伝子の発現制御機構 (2010)
  • Author(s): 能村卓慈、豊田秀一、水田順也、田代 文、倭 英司、 宮崎純一
  • Organizer: 分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2010-12-08
• [Presentation] 精子形成・卵形成初期におけるSOHLH2/SOHLH1/SP1複合体によるSohlh1遺伝子の発現制御機構 (2010)
  • Author(s): 能村卓慈、豊田秀一、水田順也、田代文、倭英司、宮崎純一
  • Organizer: 日本分子生物学会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2010-12-08
• [Book] Surface engineering to control embryonic stem cell fate. In "Methodological Advances in the Culture, Manipulation and Utilization of Embryonic Stem Cells for Basic and Practical Applications" Prof.Craig Atwood Eds, pp93-112 (2011)
  • Author(s): Mashayekhan S, Miyazaki J
  • Total Pages: 506
  • Publisher: InTech, Vienna, Austria, European Union
• [Remarks]
  • URL: http://www.med.osaka-u.ac.jp/pub/nutri/www/index.html
• [Remarks]
  • URL: http://www.med.osaka-u.ac.jp/pub/nutri/www/index.html