Understanding of the mechanism underlying the tissue-specific differentiation of high endothelial venule endothelial cells
| Project/Area Number |
22590191
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | Osaka University |
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Principal Investigator |
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 血管内皮細胞 / 細胞分化 / リンパ節 / 免疫組織 / 転写因子 / リンパ球 |
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| Research Abstract |
The high endothelial venules (HEVs) are blood vessels specifically found in lymph nodes and Peyer ’ s patches. Although HEVs express specific chemokines/adhesion molecules which mediate lymphocyte t rafficking across the high-walled endothelial cells (HEV -ECs), the mechanism regulating HEVs’ development and maintenance of the unique properties remain unclear. We performed microarray and real -time quantitative PCR analyses of HEV -ECs and non-HEV -ECs inneonatal mice mesenteric LNs, and identified five transcription factors which are over fifty times more abundantly expressed in developing HEV -ECs than in non-HEV -ECs. By immunohistochemical analysis, we found that one of them showed a restricted expression pattern in the nucleus of ECs of a substantial proportion of blood vessels oflymph nodes from E17.5 to the date of birth, which corresponds temporally to HEV -EC development. The gene knockout mice of this transcription factor showed reduced expression of several HEV -associated genes, implying the functionalcontribution of this gene to HEV -EC differentiation.
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Report
(4 results)
Research Products
(27 results)
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[Journal Article] Constitutive lymphocyte transmigration across the basal lamina of high endothelial venules is regulated by the autotaxin/lysophosphatidic acid axis.2013
Author(s)
Bai Z, Cai L, Umemoto E, Takeda A, Tohya K, Komai Y, Veeraveedu PT, Hata E, Sugiura Y, Kubo A, Suematsu M, Hayasaka H, Okudaira S, Aoki J, Tanaka T,Albers HM, Ovaa H, Miyasaka M.
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Journal Title
J. Immunol
Volume: 190
Pages: 2036-2048
Related Report
Peer Reviewed
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[Journal Article] Constitutive plasmacytoid dendritic cell migration to the splenic white pulp is cooperatively regulated by CCR7- and CXCR4-mediated signaling2012
Author(s)
Umemoto E., Otani, K Ikeno, T., Verjan Garcia N, Hayasaka H., Bai, Z., Jang MH., Tanaka T., Ueda, K., Miyasaka, M
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Journal Title
J. Immunol
Volume: 189
Pages: 191-199
Related Report
Peer Reviewed
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[Journal Article] Novel regulators of lymphocyte trafficking across high endothelial venules2011
Author(s)
Umemoto, E., Hayasaka, H., Bai, Z., Cai, L., Yonekura, S., Peng, X., Takeda, A., Tohya, K., and Miyasaka, M
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Journal Title
Crit. Rev .Immunol.
Volume: 31
Pages: 147-169
Related Report
Peer Reviewed
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[Journal Article] リンパ球トラフィキングをつかさどる新しい分子機構- 生理活性脂質産生酵素オートタキシンとその産物リゾホスファチジン酸の役割2011
Author(s)
Bai, Z., Cai, L., Umemoto E., Takeda A., Ikeno, T., Hata E., Hayasaka, H., Miyasaka, M.
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Journal Title
炎症と免疫別冊
Volume: 19
Pages: 8-13
Related Report
Peer Reviewed
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[Journal Article] Two-state conformations in the hyaluronan-binding domain regulate CD44 adhesiveness under flow condition2010
Author(s)
Ogino, S., Nishida, N., Umemoto, R., Suzuki, M., Takeda, M., Terasawa, H., Kitayama, J., Matsumoto, M., Hayasaka, H., Miyasaka, M., and Shimada, I
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Journal Title
Structure
Volume: 18
Pages: 649-656
Related Report
Peer Reviewed
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