Histochemical studies on the expression patterns and dynamical changes of carbohydrates

• Project/Area Number: 22590193
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General anatomy (including Histology/Embryology)
• Research Institution: Kyorin University
• Principal Investigator: KAWAKAMI Hayato 杏林大学, 医学部, 教授 (30146542)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 細胞組織化学 / 糖鎖 / レクチン / 組織細胞化学 / 糖脂質 / 血管内皮 / 癌細胞 / 糖尿病 / 血管内皮細胞 / 組織アレイ / 多重標識 / 糖尿病モデル動物

Research Abstract

More than fifty kinds of lectins and sugar-specific monoclonal antibodies were histochemically applied to various kinds of human and mouse tissue specimens. Organ-specific distribution of sugar moieties were demonstrated on both parenchymal cells and vascular endothelial cells. Specific distribution of endothelial cell surface carbohydrates might be an important clue to explain the mechanisms of organ-specific cancer metastasis. Specific distribution of glycolipids were also demonstrated on rat cerebrum, small intestine and kidney under various pathological states including diabetes. Histochemical approaches revealed important aspects of the dynamic changes of sugar expression on various tissues and cells.

Research Products

• [Journal Article] Novel regulation pf cardiac metabolism and homeostasis by the adrenomedullin- receptor activity-modifying protein 2 system (2013)
  • Author(s): Takahiro Yoshizawa, Takayuki Sakurai, Akiko Kamiyoshi, Yuka Ichikawa-Shindo, Hisaka Kawate, Yasuhiro Iesato, Teruhide Koyama, Ryuichi Uetake, Lei Yang, Akihiro Yamauchi, Megumu Tanaka, Yuichi Toriyama, Kyoko Igarashi, Tsutomu Nakada, Toshihide Kashihara, Mitsuhiko Yamada, Hayato Kawakami, Hiroki Nakanishi, Ryo Taguchi, Tsuyoshi Nakanishi, Hiroshi Akazawa, Takayuki Shindo
  • Journal Title: Hypertension Volume: 61 Issue: 2 Pages: 341-351
  • DOI: 10.1161/hypertensionaha.111.00647
  • Peer Reviewed
• [Journal Article] Vascular Endothelial Adrenomedullin-RAMP2 System 18 Essential for Vascular Integrity and Organ Homeostasis (2013)
  • Author(s): Teruhide Koyama. Laura Ochoa-Callejero, Takayuki Sakurai, Akiko Kamiyoshi, Yuka Ichikawa-Shindo, Nobuyoshi Iinuma, Takuma Arai, Takahiro Yoshizawa, Yasuhiro Iesato, Yang Lei, Ryuichi Uetake, Akihiro Yamauchi, Megumu Tanaka, Kyoko Igarashi, Yuichi Tbriyama, Ralf H. Adams, Hayato Kawakami, Naoki Mochizuki, Alfredo Martinez, Takayuki Shindo
  • Journal Title: Circulation Volume: 127 Issue: 7 Pages: 842-853
  • DOI: 10.1161/circulationaha.112.000756
  • Peer Reviewed
• [Journal Article] Immunopathologic co-localization of MPO, IgG, and C3 in glomeruli in human MPO-ANCA-associated glomerulonephritis (2013)
  • Author(s): Kawashima S, Arimura Y, Sano K, Kudo A, Komagata Y, Kaname S, Kawakami H, Yamada A
  • Journal Title: Clin Nephrol Volume: 79 Issue: 04 Pages: 292-301
  • DOI: 10.5414/cn107675
• [Journal Article] Mechanisms of angiogenic suppression in uteri exposed to diethylstilbestrol neonatally in tha mouse (2013)
  • Author(s): Yamashita S, Kudo A, Kawakami H, Okada Y
  • Journal Title: Biol Reprod Volume: 112
  • Peer Reviewed
• [Journal Article] Sox17 haploinsufficiency results in perinatal biliary atresia and hepatitis in C57BL/6 background mice (2013)
  • Author(s): Uemura. Mami., Ozawa A. Nagala T. Kurosawa K., Tsurukawa. K., Nobuhisa I., Taka T. Mara. K., Kudo, A., Kawakami. H., Saijoh. Y. Kurohmaru M. Kanai, Azuma. M., Kanai. Yoshiakira.
  • Journal Title: Development Volume: 140 Issue: 3 Pages: 639-648
  • DOI: 10.1242/dev.086702
  • Peer Reviewed
• [Journal Article] Expression of matrix metalloproteinases 2 and 9 and tissue inhibitors of matrix metalloproteinases 2 and 1 in the glomeruli of human glomerular diseases: the results of studies using immunofluorescence, in situ hybridization, and immunoelectron microscopy (2012)
  • Author(s): Sekiuchi M, Kudo A, Nakabayashi K,Kanai-Azuma M, Akimoto Y, Kawakami H, Yamada A
  • Journal Title: Clin Exp Nephrol Volume: 16 Issue: 6 Pages: 863-874
  • DOI: 10.1007/s10157-012-0633-3
  • NAID: 10031145918
  • Peer Reviewed
• [Journal Article] Destructive effects of butyrate on the cell envelope of Helicobacter pylori (2012)
  • Author(s): Yonezawa H, Osaki T, Hanawa T, Kurata S, Zaman C, Woo TDH, Takahashi M, Matsubara S, Kawakami H, Ochiai K, Kamiya S
  • Journal Title: J Med Microbiol Volume: 61 Issue: 4 Pages: 582-589
  • DOI: 10.1099/jmm.0.039040-0
  • Peer Reviewed
• [Journal Article] Morphological changes in diabetic kidney are associated with increased O-GlcNAcylation of cytoskeletal proteins including alpha-actinin 4 (2011)
  • Author(s): Akimoto Y, Miura Y, Toda T, Wolfert MA, Wells L, Boons G-J, Hart GW, Endo T, Kawakami H
  • Journal Title: Clin Proteomics. Volume: 8(15) Issue: 1 Pages: 1-16
  • DOI: 10.1186/1559-0275-8-15
  • Peer Reviewed
• [Journal Article] Thiazolidinediones enhance sodium-coupled bicarbonate absorption from renal proximal tubules via PPARγ-dependent nongenomic signaling (2011)
  • Author(s): Endo Y, Yamauchi T, Kubota N, Kadowaki T
  • Journal Title: CellMetabolism Volume: 13 Issue: 5 Pages: 550
  • DOI: 10.1016/j.cmet.2011.02.015
  • Peer Reviewed
• [Journal Article] Morphological changes in diabetic kidney are associated with increased O-GlcNAcylation of cytoskeletal proteins including α-actinin 4 (2011)
  • Author(s): Akimoto Y., et.al
  • Journal Title: Clin Proteom Volume: 8 Pages: 15-15
  • Peer Reviewed
• [Journal Article] Thiazolidinediones enhance sodium-coupled bicarbonate absorption from renal proximal tubules via PPAR□-dependent nongenomic signaling (2011)
  • Author(s): Endo Y., et.al
  • Journal Title: Cell Metab Volume: 13 Pages: 550-561
  • Peer Reviewed
• [Journal Article] Cyclical and patch-like GDNF distribution along the basal surface of Sertoli cells in mouse and hamster testes (2011)
  • Author(s): Sato T., et.al
  • Journal Title: Plos One Volume: 6
  • Peer Reviewed
• [Journal Article] Distribution and function of macrophage galactose-type C-type lectin 2 (MGL2/CD301b): efficient uptake and presentation of glycosylated antigens by dendritic cells. (2010)
  • Author(s): Denda-Nagai K, Aida S, Saba K, Suzuki K, Moriyama S, Oo-Puthinan S, Tsuiji M, Morikawa A, Kumamoto Y, Sugiura D, Kudo A, Akimoto Y, Kawakami H, Bovin NV, Irimura T.
  • Journal Title: J Biol Chem. Volume: 285(25) Issue: 25 Pages: 19193-204
  • DOI: 10.1074/jbc.m110.113613
  • Peer Reviewed
• [Journal Article] Maternal-effect gene Ces5/Ooep/Moep19/Floped is essential for oocyte cytoplasmic lattice formation and embryonic development at the maternal-zygotic stage transition (2010)
  • Author(s): Tashiro F, Kanai-Azuma M, Miyazaki S, Kato M, Tanaka T, Toyoda S, Yamato E,Kawakami H, Miyazaki T, Miyazaki M
  • Journal Title: Gene Cells Volume: 15 Issue: 8 Pages: 813-828
  • DOI: 10.1111/j.1365-2443.2010.01420.x
• [Journal Article] 内因性ANP・BNPによる血管新生、血管恒常性維持機構 (2010)
  • Author(s): 徳留健,岸本一郎,山原健一,新藤隆行,川上速人,白井幹康,寒川賢治
  • Journal Title: 血管 Volume: 33 Pages: 75-80
  • NAID: 10027705041
• [Journal Article] Proteomic analysis of two types of exosomes in human whole saliva. (2010)
  • Author(s): Ogawa Y, et al.
  • Journal Title: Biol.Pharm.Bull. Volume: 34 Pages: 13-23
  • Peer Reviewed
• [Journal Article] Increased apoptosis of myoblasts in Drosophila model for the Walker-Warburg syndrome. (2010)
  • Author(s): Ueyama M, et al
  • Journal Title: PLoS One Volume: 5(7)
  • Peer Reviewed
• [Journal Article] Distribution and function of macrophage galactose-type C-type lectin2 (2010)
  • Author(s): Denda-Nagai K, et al.
  • Journal Title: J.Biol.Chem. Volume: 285 Pages: 19193-19204
  • Peer Reviewed
• [Journal Article] Deletion of CDKAL1 Affects First-phase Glucose stimulated Insulin Exocytosis. (2010)
  • Author(s): Ohara-Imaizumi M., et al.
  • Journal Title: PLoS One Volume: 5:e15553
  • Peer Reviewed
• [Journal Article] In vitro antibacterial activity of Phx-3 against Helicobacter pylori. (2010)
  • Author(s): Hanawa T, et al.
  • Journal Title: Biol Pharm Bull Volume: 33 Pages: 188-191
  • Peer Reviewed
• [Journal Article] Maternal effect gene Ces5/Ooep/Moep 19/Floped is essential for oocyte cytoplasmic lattice formation and embryonic development at the maternal-zygotic stage transition. (2010)
  • Author(s): Tashiro F, et al.
  • Journal Title: Genes to Cells Volume: 15 Pages: 813-828
  • Peer Reviewed
• [Journal Article] Pre embedding electron microscopy methods for glycan localization in chemically fixed mammalian tissue using horseradish peroxidase-conjugated lectin. (2010)
  • Author(s): Akimoto Y, et al.
  • Journal Title: Methods Mol Biol Volume: 657 Pages: 217-241
• [Presentation] 糖尿病性腎症に伴うタンパク質への糖修飾異常の解析 (2013)
  • Author(s): 秋元義弘,三浦ゆり,戸田年総,福冨俊之,Hart GW,遠藤玉夫,川上速人
  • Organizer: 第118回日本解剖学会総会・全国学術集会
  • Place of Presentation: 高松
• [Presentation] 齢マウスで観察される「精上皮の波」の特徴 (2013)
  • Author(s): 宮東昭彦，松原幸枝，三浦知子，轉石小百合，川上速人
  • Organizer: 第118回日本解剖学会総会・全国学術集会
  • Place of Presentation: 高松
• [Presentation] 細胞外基質アリールスルファターゼB(ArsB)の発現と機能解析 (2012)
  • Author(s): 光永-中坪敬子, 他6名
  • Organizer: 日本動物学会中国四国支部広島県例会
  • Place of Presentation: 広島
  • Year and Date: 2012-03-03
• [Presentation] O-GlcNAc modification of proteins and diabetes (2012)
  • Author(s): Akimoto A, Miura Y, Toda T, Wolfert MA, Wells L, Boons G-J, Hart GW, Endo T,Kawakami H
  • Organizer: International Symposium on Glyco-minded Biology of Diseases as a Basis of Pharmaceutical Sciences
  • Place of Presentation: Tokyo
• [Presentation] Detection of O-GlcNAcylated proteins by glycoproteomics and in situ proximity ligation assay (PLA) (2012)
  • Author(s): Akimoto Y, Miura Y, Toda T, Wolfert MA, Wells L, Boons G-J, Hart GW, Endo T,Kawakami H
  • Organizer: 14th International Congress of Histochemistry and Cytochemistry
  • Place of Presentation: Kyoto
• [Presentation] 糖尿病角膜症に伴うタンパク質への糖(O-GlcNAc)修飾の変化 (2012)
  • Author(s): 秋元義弘,三浦ゆり,戸田年総,松原幸枝,Hart GW,遠藤玉夫,川上速人
  • Organizer: 日本顕微鏡学会第68回学術講演会
  • Place of Presentation: つくば
• [Presentation] Involvement of the murine epididymal C4BP in the reproductive system but not in the complement system (2012)
  • Author(s): Nonaka MI, Kudo A, Kawakami H, Yoshida K, Yoshida M, Nonaka M
  • Organizer: 24th International Complement Workshop
  • Place of Presentation: Chania, Crete, Greece
• [Presentation] メダカアリールスルファターゼB(ArsB)の免疫組織化学的解析 (2012)
  • Author(s): 光永敬子，秋元義弘，安井金也，楠慎一郎，山下一郎，川上速人，安増茂樹
  • Organizer: 日本動物学会第83回大会
  • Place of Presentation: 大阪
• [Presentation] Age-related changes of the wave of the seminiferous epithelium of rodent testes and effects of EDS and testosterone administration (2012)
  • Author(s): Kudo A, Matsubara S, Miura T, Koroishi S, Kawakami H
  • Organizer: 14th International Congress of Histochemistry and Cytochemistry
  • Place of Presentation: Kyoto
• [Presentation] 糖尿病角膜症に伴うタンパク質への糖（O-GlcNAc）修飾の変化 (2012)
  • Author(s): 秋元義弘，三浦ゆり，戸田年総，松原幸枝，Hart GW，遠藤玉夫，川上速人
  • Organizer: 日本顕微鏡学会第68回学術講演会
  • Place of Presentation: 大阪
• [Presentation] 糖尿病に伴う腎臓におけるα-アクチニン4の局在の変化 (2012)
  • Author(s): 秋元義弘, 他5名
  • Organizer: 第117回日本解剖学会総会・全国学術集会
  • Place of Presentation: 甲府
• [Presentation] Immunohistochemical detection of glycolipids in various tissues of rat (2011)
  • Author(s): Kawakami H
  • Organizer: 21st International Symposium on Glycoconjugates (Glyco XXI)
  • Place of Presentation: Vienna
• [Presentation] Elevated O-GlcNAc modification of proteins in diabetic kidney (2011)
  • Author(s): Akimoto Y, Miura Y, Toda T, Wolfert MA, Wells L, Boons G-J, Hart GW, Endo T, Kawakami H
  • Organizer: 21st International Symposium on Glycoconjugates (Glyco XXI)
  • Place of Presentation: Vienna
• [Presentation] DrosophilaのO-マンノース転移酵素突然変異体における筋組織の微細構造解析 (2011)
  • Author(s): 秋元義弘,上山盛夫,一宮智美,福田稔,松原幸枝,川上速人,西原祥子
  • Organizer: 日本顕微鏡学会第67回学術講演会
  • Place of Presentation: 福岡
• [Presentation] 糖尿病性角膜症に伴うタンパク質への糖修飾変化のプロテオミクスによる解析 (2011)
  • Author(s): 秋元義弘,Hart GW,三浦ゆり,戸田年総,遠藤玉夫,川上速人
  • Organizer: 第88回日本生理学会大会・第116回日本解剖学会総会・全国学術集会合同大会
  • Place of Presentation: 横浜
• [Presentation] Immunohistochemical detection of glycolipids in various tissues of rat (2011)
  • Author(s): H.Kawakami
  • Organizer: 21st International Symposium on Glycoconjugates
  • Place of Presentation: Vienna, Austria
• [Presentation] Elevated O-GlcNAc modifi cation of proteins in diabetic kidney (2011)
  • Author(s): Y.Akimoto, 他8名
  • Organizer: 21st International Symposium on Glycoconjugates
  • Place of Presentation: Vienna, Austria
• [Presentation] 糖の組織化学 (2011)
  • Author(s): 秋元義弘、川上速人
  • Organizer: 第36回組織細胞化学講習会
  • Place of Presentation: 東京
• [Presentation] 細胞内増殖性を欠損したレジオネラ強細胞毒性株の性状解析 (2011)
  • Author(s): 三宅正紀, 他8名
  • Organizer: 第23回微生物シンポジウム
  • Place of Presentation: 銚子
• [Presentation] Characterization of Legionella hyper-toxic mutants which are intracellular growth-deficient (Toxh mutants) for infectivity to host cells (2011)
  • Author(s): M.Miyake, 他7名
  • Organizer: International Union of Microbiological Societies 2011 Congress (IUMS2011)
  • Place of Presentation: Sapporo
• [Presentation] アリールスルファターゼB(ArsB)のメダカ胚形態形成における機能の解析 (2011)
  • Author(s): 光永-中坪敬子, 他5名
  • Organizer: 日本動物学会第82回年次大会
  • Place of Presentation: 旭川
• [Presentation] 百日咳菌の緊縮応答シグナル分子(p)ppGpp生合成酵素遺伝子欠損変異株の性状 (2011)
  • Author(s): 杉崎健太郎, 他6名
  • Organizer: 第94回日本細菌学会関東支部総会
  • Place of Presentation: 東京
• [Presentation] Deletion of alarmon synthetase altered physiology and biofilm formation of Bordetella pertussis (2011)
  • Author(s): K.Sugisaki, 他6名
  • Organizer: The Joint Meeting of the 17th International symposium on Gnotobiology (ISG) and the 34th Congress of Sodiety for Microbial Ecology and Disease
  • Place of Presentation: Yokohama
• [Presentation] 糖尿病性角膜症に伴うタンパク質への糖修飾変化のプロテオミクスによる解析 (2011)
  • Author(s): 秋元義弘, 他5名
  • Organizer: 第88回日本生理学会大会第116回日本解剖学会総会・全国学術集会合同大会
  • Place of Presentation: 横浜
• [Presentation] 血管内皮細胞の機能維持・破綻に関わる脱ユビキチン化酵素の機能解析 (2010)
  • Author(s): 川上速人, 他5名
  • Organizer: 第39回杏林医学会総会
  • Place of Presentation: 東京、三鷹
  • Year and Date: 2010-11-20
• [Presentation] A carcinoma-associated mucin, Muc21/epiglycanin, modulates cell adhesion and survival. (2010)
  • Author(s): Jessica Zhao, 他7名
  • Organizer: 第30回日本分子腫瘍マーカー研究会
  • Place of Presentation: 大阪
  • Year and Date: 2010-09-21
• [Presentation] In situ proximity ligation assay法による腎臓におけるO-GlcNAc修飾タンパク質の検出 (2010)
  • Author(s): 秋元義弘,三浦ゆり,戸田年総,遠藤玉夫,Hart GW,川上速人
  • Organizer: 第51回日本組織細胞化学会総会・学術集会
  • Place of Presentation: 東京
• [Presentation] 糖修飾の組織化学解析の基礎と応用 (2010)
  • Author(s): 秋元義弘,川上速人
  • Organizer: 第35回組織細胞化学講習会
  • Place of Presentation: 甲府
• [Presentation] O-GlcNAc-modification of the proteins in diabetic tissues (2010)
  • Author(s): Akimoto Y, Miura Y, Toda T, Hart GW, Endo T, Kawakami H
  • Organizer: 7th International Symposium on Glycosyltransferases
  • Place of Presentation: Tokyo
• [Presentation] Proteomic analysis of O-GlcNAc-modified proteins in diabetic kidney (2010)
  • Author(s): Akimoto Y, Miura Y, Toda T, Hart GW, Endo T, Kawakami H
  • Organizer: The 28th Naito Conference on Glycan Expression and Regulation [I]: Function and disease mechanism
  • Place of Presentation: Hayama
• [Presentation] Sox17変異マウスでのハプロ不全と新生児肝炎モデル (2010)
  • Author(s): 金井正美, 他7名
  • Organizer: 第57回実験動物学会
  • Place of Presentation: 京都
• [Presentation] 肝臓の細胞表面に局在するアリールスルファターゼ(ArsA、ArsB) (2010)
  • Author(s): 光永敬子, 他6名
  • Organizer: 第51回日本顕微鏡学会総会
  • Place of Presentation: 名古屋
• [Presentation] Proteomic analysis of O-GlcNAc-modified proteins in diabetic kidney. (2010)
  • Author(s): Yoshihiro Akimoto, 他5名
  • Organizer: The 28th Naito Conference on glycan expression and regulation [I] function and disease mechanism
  • Place of Presentation: Hayama
• [Presentation] O-GlcNAc-modification of the proteins in diabetic tissues. (2010)
  • Author(s): Yoshihiro Akimoto, 他5名
  • Organizer: 7th International Symposium on Glycosyltransferases
  • Place of Presentation: Tokyo
• [Presentation] Roles played by the galectin family genes of Caenorhabditis elegans under stress conditions. (2010)
  • Author(s): Nemoto-Sasaki Y, 他3名
  • Organizer: The 25th International Carbohydrate Symposium
  • Place of Presentation: Chiba
• [Presentation] 糖修飾の組織化学解析の基礎と応用 (2010)
  • Author(s): 秋元義弘, 他1名
  • Organizer: 組織細胞化学講習会
  • Place of Presentation: 山梨
• [Presentation] 精細管「精上皮の波」と後期精子細胞の尾部の向きとの関係 (2010)
  • Author(s): 宮東昭彦, 他3名
  • Organizer: 第51回日本組織細胞化学会総会・学術集会
  • Place of Presentation: 東京
• [Presentation] 進行性筋ジストロフィー、ウォーカー・ワールブルク症候群のモデルとなるショウジョウバエO-マンノース転移酵素の突然変異体 (2010)
  • Author(s): 上山盛夫, 他6名
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学大会合同大会
  • Place of Presentation: 神戸
• [Presentation] 哺乳類の性分化、発生過程、性成熟、季節繁殖、冬眠状態でのセルトリ細胞のニッチ能の動態 (2010)
  • Author(s): 佐藤剛, 他14名
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸
• [Book] 日本組織細胞化学会編，組織細胞化学2012．組織細胞化学の挑戦－臨床応用研究への飛躍－ (2012)
  • Author(s): 宮東昭彦，川上速人
  • Publisher: 学際企画
• [Book] 細胞質.ジュンケイラ組織学 第3版 (2011)
  • Author(s): 川上速人, 坂井建雄,川上速人
  • Publisher: 丸善
• [Book] 糖の組織化学.組織細胞化学2011 (2011)
  • Author(s): 秋元義弘,川上速人, 日本組織細胞化学会
  • Publisher: 学際企画
• [Book] 組織細胞化学2011 (2011)
  • Author(s): 川上速人(企画、編集)
  • Total Pages: 250
  • Publisher: 学際企画
• [Book] ジュンケイラ組織学 (2011)
  • Author(s): 川上速人、坂井建雄(監訳)
  • Total Pages: 498
  • Publisher: 丸善出版
• [Book] Pre-embedding electron microscopy methods for glycan localization in chemically fixed mammalian tissue using horseradish peroxidase-conjugated lectin (2010)
  • Author(s): Akimoto Y, Kawakami H
  • Publisher: ImmunoelectronMicroscopy