Histological analysis of T cell differentiation process based on hypermulticolor-hypersensitive fluorescence imaging method

• Project/Area Number: 22590194
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General anatomy (including Histology/Embryology)
• Research Institution: Juntendo University
• Principal Investigator: TSURUI Hiromichi 順天堂大学, 医学部, 助教 (40217386)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 画像分光 / 半導体色素 / サイトカインネットワーク / 超高感度蛍光イメージング / 分化特異的転写因子 / 細胞分化・組織形成 / 蛍光免疫組織染色 / 超多色蛍光イメージング / サイトカイン染色 / cytokine / multi agent system / molecular dynamics / GPGPU / MM-PBSA / TCR-pMHC相互作用 / T細胞分化 / 蛍光イメージング

Research Abstract

As the methods to evaluate chromophore-antibody conjugation state, gel-filtration chromatography, fluorescence-correlation spectroscopy, hyper-sensitive spectral imaging and high-magnification SEM were established or applied. Through those methods, two conjugation methods were established and applied to simultaneous imaging of T -bet, GATA-3, ROR-gt, FoxP3. IL-10 and IFN-a were also successfully imaged.

Research Products

• [Journal Article] Phenotype conversion from rheumatoid arthritis to systemic lupus erythematosus by introduction of Yaa mutation into FcgRIIB-deficient C57BL/6 (2013)
  • Author(s): Kawano S, Lin Q, Amano H, Kaneko T, Nishikawa K, Tsurui H, Tada N, Nishimura H,Takai T, Shirai T, Takasaki Y and Hirose S
  • Journal Title: mice. Volume: 43 Issue: 3 Pages: 770-778
  • DOI: 10.1002/eji.201243057
  • Peer Reviewed
• [Journal Article] Lissencephaly with marked ventricular dilation, agenesis of corpus callosum (2013)
  • Author(s): Akihisa Okumura , Masaharu Hayashi, Hiromichi Tsurui, Yoko Yamakawa, Shinpei Abe, Takahiro Kudo, Ryuyo Suzuki, Toshiaki Shimizu, Keiko Shimojima, Toshiyuki Yamamoto
  • Journal Title: and cerebellar hypoplasia caused by TUBA1A mutation Brain and Developoment Volume: Vol35 Issue 3 Issue: 3 Pages: 274-279
  • DOI: 10.1016/j.braindev.2012.05.006
  • Peer Reviewed
• [Journal Article] Transgenic overexpression of G5PR that is normally augmented in centrocytes impairsthe enrichment of high-affinity antigen-specific B cells, increases peritoneal B-1a cells, and induces autoimmunity in aged female mice (2012)
  • Author(s): hi H, Ohtsuji M, Tsurui H, Hirose S, and Sakaguchi N
  • Journal Title: J. Immunol Volume: 189 Issue: 3 Pages: 1193-1201
  • DOI: 10.4049/jimmunol.1102774
  • Peer Reviewed
• [Journal Article] Presumptive role of 129 strain-derived Sle16 locus in rheumatoid arthritis in a new mouse model with Fcy receptor type Iib-deficient C57BL/6 genetic background. (2011)
  • Author(s): Sato-Hayashizaki A, Ohtsuji M, Lin Q, Hou R, Ohtsuji N, Nishikawa K, Tsurui H, Sudo K, Ono M, Izui S, Shirai T, Takai T, Nishimura H, Hirose S
  • Journal Title: Arthritis & Rheumatism Volume: 63 Issue: 10 Pages: 2930-2938
  • DOI: 10.1002/art.30485
  • Peer Reviewed
• [Journal Article] Inhibitory IgG Fc receptor promoter region polymorphism is a key genetic element for murine systemic lupus erythematosus (2010)
  • Author(s): Lin Q Hou R, Sato A, Ohtsuji M, Ohtsuji N, Nishikawa K, Tsurui H, Amano H, Amano E, Sudo K, Nishimura H, Shirai T, Hirose S
  • Journal Title: J Autoimmunity Volume: 34 Issue: 4 Pages: 356-363
  • DOI: 10.1016/j.jaut.2009.08.011
• [Journal Article] FTY720 exerts a survival advantage through the prevention of end-stage glomerular inflammation in lupus-prone BXSB mice (2010)
  • Author(s): Ando S, Amano H, Amano E, Minowa K, Watanabe T, Nakano S, Nakiri Y, Morimoto S, Tokano Y, Lin Q, Hou R, Ohtsuji M, Tsurui H, Hirose S, Takasaki Y.
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 394 Issue: 3 Pages: 804-810
  • DOI: 10.1016/j.bbrc.2010.03.078
• [Journal Article] Phagocytosis specific autofluorescence in marginal metallophilic macrophage and marginal zone macrophage (2010)
  • Author(s): Tsurui H, Hirose S
  • Journal Title: International Immunology Volume: 22 Sup 1 part3 Pages: 40-40
• [Presentation] Thorough characterization of TCR-pMHC binding free energy estimated by string model and Miyazawa-Jernigan matrix (2012)
  • Author(s): TSURUI Hiromichi, HIROSE Sachiko
  • Organizer: 第41回 日本免疫学会総会・学術集会
  • Place of Presentation: 神戸
• [Presentation] Phenotype conversion from RA to SLE in FcγRIIB-deficient B6 mice by Yaa mutation (2012)
  • Author(s): KAWANO Shinya, AMANO Hirofumi, LIN Qingshun, KANEKO Toshiyuki, NISHIKAWA Keiko, OKAZAKI Hideki, TSURUI Hiromichi, NISHIMURA Hiroyuki, SHIRAI Toshikazu, TAKASAKI Yoshinari, HIROSE Sachiko.
  • Organizer: 第41回 日本免疫学会総会・学術集会
  • Place of Presentation: 神戸
• [Presentation] The role of SAP-signal in SLE (2012)
  • Author(s): Lin Qingshun, TSURUI Hiromichi, NISHIKAWA Keiko, OKAZAKI Hideki, OHTSUJI Mareki, NISHIMURA Hiroyuki, ONO Masao, SHIRAI Toshikazu, HIROSE Sachiko.
  • Organizer: 第41回 日本免疫学会総会・学術集会
  • Place of Presentation: 神戸
• [Presentation] A novel locus of B6 mice on chromosome 12 plays a role in common process shared by SLE, RA, and Sjögren syndrome (2012)
  • Author(s): Kaneko Toshiyuki, AMANO Hirofumi, OHTSUJI Mareki, NISHIKAWA K, KAWANO S, OHTSUJI N, Lin Q, OKAZAKI H, TSURUI H, NISHIMURA H, SHIRAI T, TAKASAKI Y, HIROSE Sachiko.
  • Organizer: 第41回 日本免疫学会総会・学術集会
  • Place of Presentation: 神戸
• [Presentation] Characterization of TCR-pMHC interaction based on string model and Miyazawa-Jernigan matrix (2011)
  • Author(s): 鶴井博理・広瀬幸子
  • Organizer: 日本免疫学会学術集会
  • Place of Presentation: 千葉・幕張メッセ
  • Year and Date: 2011-11-28
• [Presentation] Phagocytosis specific autofluorescence in marginal metallophilic macrophage and marginal zone macrophage (2010)
  • Author(s): Tsurui H, Hirose S
  • Organizer: 22th International Congress of Immunology
  • Place of Presentation: Kobe
  • Year and Date: 2010-08-23
• [Book] Molecular Dynamics - Studies of Synthetic and Biological Macromolecules (2012)
  • Author(s): TSURUI H and TAKAHASHI T.
  • Publisher: InTech
• [Book] Practical Estimation of TCR-pMHC binding free-energy based on the dielectric model and the Coarse-grained model in “Molecular Dynamics - Studies of Synthetic and Biological Macromolecules” (2012)
  • Author(s): Tsurui Hiromichi and Takahashi Takuya
  • Total Pages: 29
  • Publisher: InTech
• [Book] Molecular Dynamics Book 2, ISBN979-953-307-865-5のうちの1章Practical Estimation of TCR-pMHC Binding Free-Energy Based on the Dielectric Model and the Course-grained Modelを担当 (2012)
  • Author(s): Hiromichi Tsurui, Takuya Takahashi
  • Total Pages: 432
  • Publisher: InTech