Analysis of microvillous formation mechanism and cell adhesion regulation by mucin-type glycoproteins

• Project/Area Number: 22590198
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General anatomy (including Histology/Embryology)
• Research Institution: National Institute of Advanced Industrial Science and Technology
• Principal Investigator: TACHIBANA Kouichi 独立行政法人産業技術総合研究所, 健康工学部門, 主任研究員 (80216986)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: ムチン型糖蛋白質 / 細胞接着 / 微絨毛 / ERM 蛋白質 / リン酸化 / 細胞骨格 / 細胞球状化 / ERM蛋白質 / 細胞・組織 / 生物物理 / ムチン / CD43 / マイクロビライ

Research Abstract

We found expression of leukocyte surface mucin such as CD43 or CD34 resulted in microvillus formation and spherical cell shape in HEK293T cells. These phenomena were induced by the inhibition of cell adhesion by mucins via activation of ERM proteins. Mean

Research Products

• [Journal Article] Formation of microvilli and phosphorylation of ERM family proteins by CD43, a potent inhibitor for cell adhesion (2011)
  • Author(s): Yamane J, Ohnishi H, Sasaki H, Narimatsu H, Ohgushi H, Tachibana K
  • Journal Title: Cell Adhesion & Migration Volume: 5 Issue: 2 Pages: 119-132
  • DOI: 10.4161/cam.5.2.13908
  • Peer Reviewed
• [Journal Article] Formation of microvilli and phosphorylation of ERM family proteins by CD43, a potent inhibitor for cell adhesion. (2011)
  • Author(s): Junko Yamane, et al.
  • Journal Title: Cell Adhesion & Migration Volume: 5 Pages: 119-132
  • Peer Reviewed
• [Presentation] 細胞接着阻害因子CD43 によるマイクロビライ形成機序の解析 (2010)
  • Author(s): 立花宏一、山根順子
  • Organizer: CD43. BMB2010
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-07
• [Presentation] Mechanisms of Microvillous Formation by the cell adhesion inhibitor, CD43. (2010)
  • Author(s): 立花宏一
  • Organizer: BMB2010
  • Place of Presentation: 神戸国際会議場(兵庫県)
  • Year and Date: 2010-12-07
• [Presentation] Cell surface sialomucin, CD43, induces Microvillous formation via inhibition of cell adhesion (2010)
  • Author(s): Tachibana K, Yamane J
  • Organizer: 日本生物物理学会年会
  • Place of Presentation: 仙台
  • Year and Date: 2010-09-22
• [Presentation] Cell surface sialomucin, CD43, induces Microvillous formation via inhibition of cell adhesion. (2010)
  • Author(s): 立花宏一
  • Organizer: 日本生物物理学会
  • Place of Presentation: 東北大学工学部(宮城県)
  • Year and Date: 2010-09-22