Analysis of the voltage-dependent calcium channelβsubunit in response to sympathetic nerve dependent phosphorylation

• Project/Area Number: 22590200
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General physiology
• Research Institution: Hirosaki University (2012); University of Miyazaki (2010-2011)
• Principal Investigator: MURAKAMI Manabu 弘前大学, 医学(系)研究科(研究院), 教授 (80302090)
• Co-Investigator(Kenkyū-buntansha): ONO Kyoichi 秋田大学, 医学(系)研究科(研究院), 教授 (70185635) ; OHBA Takayoshi 秋田大学, 医学(系)研究科(研究院), 助教 (80431625) ; NEMOTO Takayuki 宮崎大学, 医学部, 助教 (90506833) ; YANAGITA Toshihiko 宮崎大学, 医学部, 准教授 (60295227) ; IIGIMA Toshihiko 秋田大学, 医学(系)研究科(研究院), 名誉教授 (30004724)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 生体膜 / チャネル / トランスポーター / 能動輸送 / カルシウム / 交感神経 / カテコールアミン / 電気生理 / マウス / リン酸化 / 副腎髄質 / STIM1

Research Abstract

Mice with β_2 subunit-null mutations exhibited reduced cardiac muscle contraction in response to isoproterenol, and did not exhibit significant increases in Ca^2+ channel currents upon PKA-dependent phosphorylation. These findings indicate that the primary target for PKA by the sympathetic nervous system might be the subunit

Research Products

• [Journal Article] Involvement of the orexin system in adrenal sympathetic regulation (2013)
  • Author(s): Nemoto T, Toyoshima-Aoyama F, Ueda Y, Ohba T, Yanagita T, Watanabe H, Shirasaka T, Tsuneyoshi I, Ishida Y, Hirota K, Sawaguchi A, Murakami M
  • Journal Title: Pharmacology Volume: (In press)
  • Peer Reviewed
• [Journal Article] Agonist-mediated activation and trafficking of the RAMP-based Family B GPCRs. (2013)
  • Author(s): Kuwasako K,Kitamura K, Nagata S, Murakami M, Kato J.
  • Journal Title: Curr Protein and Peptide Science Volume: in press
  • Peer Reviewed
• [Journal Article] Endothelin-1-induced down-regulation of NaV1.7 expression in adrenal chromaffin cells: attenuations of catecholamine secretion and tau dephosphorylation. (2013)
  • Author(s): Nemoto T, Yanagita T, Maruta T, Sugita C, Satoh S, Kanai T, Wada A, Murakami M.
  • Journal Title: FEBS letters Volume: in press
  • Peer Reviewed
• [Journal Article] Involvement of the orexin system in adrenal sympathetic regulation. (2013)
  • Author(s): Nemoto T, Toyoshima-Aoyama F, Ueda Y, Ohba T, Yanagita T, Watanabe H, Shirasaka T, Tsuneyoshi I, Ishida Y, Hirota K, Sawaguchi A, Murakami M.
  • Journal Title: Pharmacology Volume: in press
  • Peer Reviewed
• [Journal Article] Agonist-mediated activation and trafficking of the RAMP-based Family B GPCRs (2012)
  • Author(s): Kuwasako K,Kitamura K, Nagata S, Murakami M, Kato J
  • Journal Title: Curr Protein and Peptide Science Volume: (In press)
  • Peer Reviewed
• [Journal Article] Novel PKCα-mediated phosphorylation site(s) on cofilin and their potential role in terminating histamine release (2012)
  • Author(s): Sakuma et al.,
  • Journal Title: Molecular Biology of the Cell Volume: 23 Pages: 3707-3721
  • Peer Reviewed
• [Journal Article] Encouragement of role‐play : effectiveness of the student role‐play in the pharmacology education at the school of Medicine, Dentistry and Pharmaceutical Sciences (2012)
  • Author(s): Okamoto et al.,
  • Journal Title: J. Mol. Cell Cardiol. Volume: 52 Pages: 988-997
  • Peer Reviewed
• [Journal Article] Store-operated Ca^2+ entry suppresses distention-induced ATP release from urothelium (2011)
  • Author(s): Matsumoto-Miyai K., Kagase A., Yamada E., Yoshizumi M., Murakami M., Ohba T., Kawatani M
  • Journal Title: American Journal of Physiology-Renal Physiology Volume: F716-720
  • Peer Reviewed
• [Journal Article] Transcriptional up-regulation of cell surface NaV1.7 sodium channels by insulin-like growth factor-1 via inhibition of glycogen synthase kinase-3 β in adrenal chromaffin cells : enhancement of 22^Na^+ influx, 45^Ca^2+ influx and catecholamine secretion (2011)
  • Author(s): Yanagita, T., Satoha, S., Uezono, Y., Matsuo, K., Nemoto, T., Maruta, T., Yoshikawa, N., Iwakirid, T., Minami, K., and Murakami, M
  • Journal Title: Neuropharmacology Volume: 61 Pages: 1265-1274
  • Peer Reviewed
• [Journal Article] Insulin-induced neurite-like process outgrowth : Acceleration of tau protein synthesis via a phosphoinositide 3-kinase-mammalian target of rapamycin pathway (2011)
  • Author(s): Nemoto T, Yanagita T, Satoh S, Maruta T, Kanai T, Murakami M, Wada A
  • Journal Title: Neurochem Int Volume: 59 Pages: 880-888
  • Peer Reviewed
• [Journal Article] N-type voltage-dependent calcium channels in the sympathetic nervous system (2011)
  • Author(s): Murakami M
  • Journal Title: Hirosaki Medical Journal Volume: 62
  • Peer Reviewed
• [Journal Article] Store-operated Ca^<2+> entry suppresses distention-induced ATP release from urothelium (2011)
  • Author(s): Matsumoto-Miyai K, Kagase A, Yamada E, Yoshizumi M, Murakami M, Ohba T, Kawatani M
  • Journal Title: American Journal of Physiology-Renal Physiology
  • Peer Reviewed
• [Journal Article] Transcriptional up-regulation of cell surface NaV1.7 sodium channels by insulin-like growth factor-1 via inhibition of glycogen synthase kinase-3β in adrenal chromaffin cells : enhancement of ^<22>Na^+ influx, ^<45>Ca^<2+> influx and catecholamine secretion (2011)
  • Author(s): Yanagita T, Satoha S, Uezono Y, Matsuo K, Nemoto T, Maruta T, Yoshikawa N, Iwakirid T, Minami K, Murakami M
  • Journal Title: Neuropharmacology Volume: 61 Pages: 1265-74
  • Peer Reviewed
• [Journal Article] Store-operated Ca^<2+> entry suppresses distention-induced ATP release from urothelium. (2011)
  • Author(s): Matsumoto-Miyai K, et al.
  • Journal Title: American Journal of Physiology-Renal Physiology Volume: 300
  • Peer Reviewed
• [Journal Article] Behavioral and neurochemical characterization of mice deficient in the N-type Ca^2+ channel α1B subunit (2010)
  • Author(s): Nakagawasai, O., Onogi, H., Mitazaki, S., Sato, A., Watanabe, K., Saito, H., Murai, S., Nakaya, S., Murakami, M., Takahashi, E., Tan-No, K., Takeshi Tadano, T
  • Journal Title: Behavioural Brain Research Volume: 208 Pages: 224-230
  • Peer Reviewed
• [Journal Article] Decreased calcium channel currents and facilitated epinephrine release in the Ca^2+ channel β3 subunit-null mice (2010)
  • Author(s): Ohta, T., Ohba, T., Suzuki, T., Watanabe, H., Sasano, H., Murakami, M
  • Journal Title: Biochemical & Biophysical Research Communications Volume: 394 Pages: 464-469
  • Peer Reviewed
• [Journal Article] Klotho is associated with VEGF receptor-2 and the transient receptor potential canonical-1 Ca^2+ channel to maintain endothelial integrity (2010)
  • Author(s): Kusaba, T., Okigaki, M., Matui, A., Murakami, M., Ishikawa, K., Kimura, T., Sonomura, K., Adachi, Y., Shibuya, M., Shirayama, T., Tanda, S., Hatta, T., Sasaki, S., Moria, Y., and Matsubara, H
  • Journal Title: Proceedings of the National Academy of Sciences of the United States of America Volume: 107 Pages: 19308-19313
  • Peer Reviewed
• [Journal Article] Fluvastatin protects vascular smooth muscle cells against oxidative stress through the Nrf2-dependent antioxidant pathway (2010)
  • Author(s): Makabe, S., Takahashi, Y., Watanabe, H., Murakami, M., Ohba, T., and Ito, H
  • Journal Title: Atherosclerosis Volume: 213 Pages: 377-384
  • Peer Reviewed
• [Journal Article] Decreased calcium channel currents and facilitated epinephrine release in the Ca^<2+> channel β3 subunit-null mice. (2010)
  • Author(s): Ohta T, et al.
  • Journal Title: Biochemical & Biophysical Research Communications Volume: 394 Pages: 464-469
  • Peer Reviewed
• [Journal Article] Klotho is associated with VEGF receptor-2 and the transient receptor potential canonical-1 Ca^<2+> channel to maintain endothelial integrity. (2010)
  • Author(s): Kusaba T, et al.
  • Journal Title: Proceedings of the National Academy of Sciences of the United States of America Volume: 107 Pages: 19308-19313
  • Peer Reviewed
• [Journal Article] Fluvastatin protects vascular smooth muscle cells against oxidative stress through the Nrf2-dependent antioxidant pathway. (2010)
  • Author(s): Makabe S, et al.
  • Journal Title: Atherosclerosis Volume: 213 Pages: 377-384
  • Peer Reviewed
• [Journal Article] Behavioral and neurochemical characterization of mice deficient in the N-type Ca^<2+> channel α1B subunit. (2010)
  • Author(s): Nakagawasai O, et al.
  • Journal Title: Behavioural Brain Research Volume: 208 Pages: 224-230
  • Peer Reviewed
• [Presentation] Encouragement of role ‐ play : effectiveness of the student role‐play in the pharmacology education at the school of Medicine, Dentistry and Pharmaceutical Sciences (2013)
  • Author(s): Yanagita et al
  • Organizer: 日本薬理学会年会
  • Place of Presentation: 福岡
• [Presentation] Trial of practical pharmacotherapy education using role‐play with third‐year medical students act as physicians and patients (2013)
  • Author(s): Yanagita et al
  • Organizer: 日本薬理学会年会
  • Place of Presentation: 福岡
• [Presentation] Regulation of homodimerization of insul in receptor and insulin‐like growth factor‐1 receptor by heat shock protein 90 oligomerization (2013)
  • Author(s): Yanagita et al
  • Organizer: 日本薬理学会年会
  • Place of Presentation: 福岡
• [Presentation] Endothelin‐1‐induced down‐regulation of Na_V 1.7expression in adrenal chromaffin cells : attenuation of catecholamine secretion and tau dephosphorylation (2013)
  • Author(s): Nemoto et al
  • Organizer: 日本薬理学会年会
  • Place of Presentation: 福岡
• [Presentation] Elevated plasma factor VIII enhances venous thrombus growth in rabbit (2013)
  • Author(s): Sugita C, Yanagita T, Nemoto T, Yamashita A, Matsuura Y, Iwakiri T, Okuyama N, Asada Y, Murakami M
  • Organizer: 日本薬理学会年会
  • Place of Presentation: 福岡
• [Presentation] Encouragement of role‐play : effectiveness of the student role‐play in the pharmacology education at the school of Medicine, Dentistry and Pharmaceutical Sciences (2013)
  • Author(s): Yanagita et al.
  • Organizer: 日本薬理学会年会
  • Place of Presentation: 福岡
• [Presentation] Regulation of homodimerization of insulin receptor and insulin‐like growth (2013)
  • Author(s): Yanagita et al.
  • Organizer: 日本薬理学会年会
  • Place of Presentation: 福岡
• [Presentation] Endothelin‐1‐induced down‐regulation of Nav1.7 expression in adrenal chromaffin cells : attenuation of catecholamine secretion and tau dephosphorylation. (2013)
  • Author(s): Nemoto et al.
  • Organizer: 日本薬理学会年会
  • Place of Presentation: 福岡
• [Presentation] インスリンによるtau蛋白合成促進 (2011)
  • Author(s): 根本隆行, 柳田俊彦, 村上学
  • Organizer: 日光シンポジウムトランスポーター研究会
  • Place of Presentation: 日光
  • Year and Date: 2011-12-17
• [Presentation] Insulin-induced neurite-like process outgrowth (2011)
  • Author(s): Nemoto T, Yanagita T, Murakami M
  • Organizer: Society for Neuroscience
  • Place of Presentation: Washington DC, USA
  • Year and Date: 2011-11-16
• [Presentation] 電位依存性カルシウムチャネルと交感神経 (2011)
  • Author(s): 村上 学
  • Organizer: 第5回トランスポーター研究会九州部会
  • Place of Presentation: 宮崎 (招待講演)
  • Year and Date: 2011-09-17
• [Presentation] 電位依存性カルシウムチャネルと交感神経 (2011)
  • Author(s): 村上学
  • Organizer: 第5回トランスポーター研究会九州部会
  • Place of Presentation: 宮崎(招待講演)
  • Year and Date: 2011-09-17
• [Presentation] インスリンによる tau 蛋白合成促進 (2011)
  • Author(s): 根本 隆行, 柳田 俊彦, 村上 学
  • Organizer: 日光シンポジウムトランスポーター研究会
  • Place of Presentation: 日光
• [Presentation] Insulin-induced neurite-like process outgrowth (2011)
  • Author(s): Nemoto T, Yanagita T, Murakami M
  • Organizer: Society for Neuroscience, 40th Annual Meeting
  • Place of Presentation: Washington DC, USA,USA.
• [Presentation] 電位依存性カルシウムチャネルβ欠損マウスにおけるエピネフリン分泌亢進 (2011)
  • Author(s): 村上学, 他
  • Organizer: 第84回日本薬理学会年会
  • Place of Presentation: (誌上開催)
• [Presentation] インスリン受容体発現のHsp90とコシャペロンによる制御 (2011)
  • Author(s): 柳田俊彦, 他
  • Organizer: 第84回日本薬理学会年会
  • Place of Presentation: (誌上開催)
• [Presentation] インスリン受容体シグナルにおける正と負の発現調節機構 (2011)
  • Author(s): 根本隆行, 他
  • Organizer: 第84回日本薬理学会年会
  • Place of Presentation: (誌上開催)
• [Presentation] 新生仔ラットの心筋細胞の肥大形成におけるSTIM1の役割 (2011)
  • Author(s): 大場貴喜, 他
  • Organizer: 第84回日本薬理学会年会
  • Place of Presentation: (誌上開催)
• [Presentation] Decreased calcium channel currents and facilitated epinephrine release in the Ca^<2+> channel β3 subunit-null mice. (2010)
  • Author(s): Murakami M, et al.
  • Organizer: Society for Neuroscience, 40th Annual Meeting
  • Place of Presentation: San Diego, USA
  • Year and Date: 2010-11-16
• [Presentation] Decreased calcium channel currents and facilitated epinephrine release in the Ca^2+ channel β3 subunit-null mice (2010)
  • Author(s): Murakami M et al
  • Organizer: Society for Neuroscience, 40th Annual Meeting
  • Place of Presentation: San Diego, USA