| Project/Area Number |
22590205
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General physiology
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| Research Institution | Shiga University of Medical Science |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KOJIMA Akiko 滋賀医科大学, 医学部, 助教 (50447877)
OMATSU Mariko 滋賀医科大学, 医学部, 准教授 (80161397)
DING Wei-guang 滋賀医科大学, 医学部, 助教 (80242973)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 生体膜 / チャネル / トランスポーター / 能動輸送 / 心筋虚血再灌流傷害 / TRPCチャネル / Ca2+過負荷 / 細胞過拘縮 / ランゲンドルフ灌流法 / 2-APB / LaCl3 / 再疎通療法 / 活性酸素種 / 過酸化水素 / 共焦点レーザ顕微鏡 / 細胞内Ca^<2+>イメージング / 過拘縮 / パッチクランプ法 / Ca^<2+>パラドックス / マウス心室筋細胞 / fluo-3 / 虚血再灌流傷害 / Ca^<2+>過負荷 |
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| Research Abstract |
Ca2+overload has been implicated in the pathogenesis of ischemia/reperfusion injury in the heart. The present investigation used Langendorff perfusion method of mouse hearts and fluorescence Ca2+imaging of mouse ventricular myocytes and revealed that the transient receptor potential canonical (TRPC) channels mediate the Ca2+entry responsible for Ca2+overload during the reperfusion of ischemic myocardium. These observations indicate that TRPC channel blockade could provide potential new avenues to develop strategies for protecting the heart during reperfusion of ischemic myocardium.
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