Functional interaction between the plasma membrane Ca-ATPase and the Na/Ca exchanger in reverse EC coupling in heart cells.
| Project/Area Number |
22590207
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General physiology
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| Research Institution | Saga University |
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Principal Investigator |
SHIOYA Takao 佐賀大学, 医学部, 助教 (20253594)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 心筋細胞 / カルシウムシグナリング / 心筋活動電位 / リバースECカップリング / Na/Ca交換 / 細胞膜Caポンプ / 心筋の生理 / パッチクランプ / Ｎａ／Ｃａ交換電流 / 細胞内マイクロドメイン / 細胞内カルシウム / コンピュータシミュレーション / Na/Ca交換電流 / 細胞膜Caポンプ電流 |
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| Research Abstract |
Heart cells have two distinct Ca-transporters on their plasma membrane: the plasma membrane Ca-ATPase (PMCA) and the Na/Ca exchanger (NCX). This study sought for the functional cooperation between them, using the whole-cell clamp recording from mouse heart cells and the computer simulation of local Ca2+concentration profile. This study concludes: 1) the PMCA in heart cells maintains a low intracellular Ca2+level nearby itself, and regulates the NCX using the local Ca2+level as the signal medium.2) This NCX regulation suppresses the reverse EC coupling, preventing the excess prolongation of the action potential.
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Report
(4 results)
Research Products
(27 results)
