| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2013: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000)
Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Outline of Final Research Achievements |
Enhanced cell proliferation of synthetic vascular smooth muscle cells (VSMC) are due to constantly activated cell cycle progression. Entry of Ca2+ are necessary both for the progression of G1 to S phase and for the M phase. We aimed to identify Ca2+ channels, which were involved in the regulation of cell cycle. The cell proliferation of VSMC was significantly inhibited by treatment with nifedipine. Exposure of VSMC to high KCl (90 mM) also significantly inhibited the cell proliferation. When beta subunit of L-type Ca2+ channel was constitutively expressed under the control by tetracyclin, the beta subunit protein was only localized in the nucleus. Microarray analysis demonstrated that 35 genes were significantly upregulated. These results suggest that the regulation of cell proliferation of VSMC might be determined by the specific pathway of Ca2+ entry and that the beta subunit in nucleus plays an important role by regulating many genes that involves cytosolic Ca2+ homeostasis.
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