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Estrogen-induced signaling mechanisms involed in the establishment of the sexual dimorphysm of the rat brain

Research Project

Project/Area Number 22590214
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General physiology
Research InstitutionNippon Medical School

Principal Investigator

KIYAMA Yuko  日本医科大学, 医学部, 准教授 (60234390)

Co-Investigator(Renkei-kenkyūsha) 木山 亮一   (00240739)
Project Period (FY) 2010-10-20 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2012: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywordsシグナル伝達 / エストロゲン / マイクロアレイ / 脳の性分化 / ラット / 能の性分化 / エピジェネテック
Research Abstract

In the rat brain, the sexually dimorphic nucleus (SDN) of the medial preoptic area (POA) is a major site for controlling sexual dimorphic behavior and physiological functions. The dimorphism is formed by the exposure to 17beta;-estradiol (E2) during the critical period, which begins around embryonic day 18. In order to explore key factors and mechanisms involved in the establishment of sex differences in response to E2, we prepared total RNA from brain slices containing SDN from female rats treated with E2 at birth, which was subjected to DNA microarray. The expression of the gene for protein kinase C-delta; (PKC-delta;) was significantly up-regulated by E2. We then examined the downstream effectors of PKC-delta; by Western blotting and immunohistochemistry, and found the involvement of the PKC-delta;/Rac1/LIMK1/cofilin pathway. This work demonstrated that cell migration enhanced by actin dynamics is a cue to create the sexual dimorphism in POA of the rat brain.

Report

(5 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Annual Research Report
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (16 results)

All 2013 2012 2011 2010 Other

All Journal Article (4 results) (of which Peer Reviewed: 4 results) Presentation (11 results) Remarks (1 results)

  • [Journal Article] Estrogen-induced cell signaling in the sexually dimorphic nucleus of the rat preoptic area: potential involvement of cofilin in actin dynamics for cell migration2013

    • Author(s)
      Wada-Kiyama Y, Suzuki C, Hamada T, Rai D, Kiyama R, Kaneda M, Sakuma Y
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 434(2) Issue: 2 Pages: 287-292

    • DOI

      10.1016/j.bbrc.2013.02.117

    • Related Report
      2013 Annual Research Report 2013 Final Research Report 2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Distribution and development of P2Y_1-purinoceptors in the mouse retina2013

    • Author(s)
      Rai D., Ishii T., Imada H., Wada-Kiyama Y., Kiyama R., Miyachi E., Kaneda M.
    • Journal Title

      J. Molecular Histology

      Volume: vol.44 Issue: 6 Pages: 639-644

    • DOI

      10.1007/s10735-013-9525-4

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] A conserved regulatory element in the mammalian beta-globin promoters2011

    • Author(s)
      Kiyama, R., Wada-Kiyama, Y.
    • Journal Title

      J.Mol.Evol.

      Volume: 73 Issue: 3-4 Pages: 101-108

    • DOI

      10.1007/s00239-011-9459-y

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] A conserved regulatory element in the mammalian β-globin promoters2011

    • Author(s)
      Kiyama R., Wada-Kiyama Y.
    • Journal Title

      J.Mol.Evol.

      Volume: 73 Pages: 101-108

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Presentation] Actin dynamics in the sexually dimorphic nucleus of the rat preoptic area during the critical period2012

    • Author(s)
      木山裕子, 他4名
    • Organizer
      第89回日本生理学学会大会
    • Place of Presentation
      長野県
    • Year and Date
      2012-03-31
    • Related Report
      2011 Annual Research Report
  • [Presentation] Actin dynamics in the sexually dimorphic nucleus of the rat preoptic area during the critical periods2012

    • Author(s)
      Wada-Kiyama Y., Suzuki C., Hamada T., Kiyama R., Sakuma Y.
    • Organizer
      第89回日本生理学学会大会
    • Place of Presentation
      長野県
    • Related Report
      2013 Final Research Report
  • [Presentation] Estradiol-induced phosphorylation status of cofilin cascade and the establishment of the male-phenotype of the sexually dimorphic nucleus of the rat preoptic area2011

    • Author(s)
      Wada-Kiyama Y., 他4名
    • Organizer
      The 41th Annual Meeting of the Society for Neuroscience
    • Place of Presentation
      Washington DC, MD, USA
    • Year and Date
      2011-11-13
    • Related Report
      2011 Annual Research Report
  • [Presentation] Actin dynamics involved in sexual dimorphism of the preoptic area in the rat brain by estrogen2011

    • Author(s)
      Kawashima M., 他6名
    • Organizer
      日米脳
    • Place of Presentation
      Atlanta, GA, USA
    • Year and Date
      2011-10-23
    • Related Report
      2011 Annual Research Report
  • [Presentation] Signaling of estrogen to actin dynamics via cofilin controls sexually dimorphic formation of the rat preoptic area2011

    • Author(s)
      Wada-Kiyama Y., Suzuki C., Hamada T., Kiyama R., Sakuma Y.
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      神奈川県横浜
    • Related Report
      2013 Final Research Report 2011 Annual Research Report
  • [Presentation] Estradiol-induced phosphorylation status of cofilin cascade and the establishment of the male-phenotype of the sexually dimorphic nucleus of the rat preoptic area2011

    • Author(s)
      Wada-Kiyama Y., Suzuki C., Hamada T., Kiyama R., Sakuma Y.
    • Organizer
      The Annual Meeting of the Society for Neuroscience
    • Place of Presentation
      USA
    • Related Report
      2013 Final Research Report
  • [Presentation] Actin dynamics involved in sexual dimorphism of the preoptic area in the rat brain by estrogen2011

    • Author(s)
      Kawashima M., Kamijyo K., Suzuki C., Hamada T., Kiyama R., Wada-Kiyama Y., Sakuma Y.
    • Organizer
      US-JAPAN Brain Research Cooperative Program Workshop on Prosocial Behavior at Emory University
    • Place of Presentation
      USA
    • Related Report
      2013 Final Research Report
  • [Presentation] Estradiol-induced signaling involved in the sexual differentiation of the rat preoptic area.2010

    • Author(s)
      木山裕子, 等5名
    • Organizer
      日本分子生物学会年会・日本生化学会大会 合同大会
    • Place of Presentation
      神戸ポートアイランド(兵庫県)
    • Year and Date
      2010-12-07
    • Related Report
      2010 Annual Research Report
  • [Presentation] 雌ラット脳の性分化臨界期における視索前野のエストロゲン応答因子2010

    • Author(s)
      木山裕子, 等5名
    • Organizer
      日本生理学会大会
    • Place of Presentation
      いわて県民情報交流センター(岩手県・盛岡市)
    • Year and Date
      2010-05-20
    • Related Report
      2010 Annual Research Report
  • [Presentation] Estradiol-induced signaling involved in the sexual differentiation of the rat preoptic area2010

    • Author(s)
      Suzuki C., Hamada T., Kiyama R., Sakuma Y., Wada-Kiyama Y.
    • Organizer
      第33回日本分子生物学会年会
    • Place of Presentation
      兵庫県神戸
    • Related Report
      2013 Final Research Report
  • [Presentation] Estrogen-responsive genes in the preoptic area of the female rat during the critical period for sexual differentiation of the brain2010

    • Author(s)
      Wada-Kiyama Y., Suzuki C., Hamada T., Kiyama R., Sakuma Y.
    • Organizer
      第87回日本生理学学会大会
    • Place of Presentation
      岩手県
    • Related Report
      2013 Final Research Report
  • [Remarks]

    • URL

      http://www.h-ic.664u.ne.jp/~seiri/

    • Related Report
      2013 Final Research Report

URL: 

Published: 2010-11-30   Modified: 2019-07-29  

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