| Project/Area Number |
22590217
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General physiology
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| Research Institution | Osaka Medical College |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
山路 純子 (田代 純子) 大阪医科大学, 医学部, 講師 (40340559)
白岩 有桂 大阪医科大学, 医学部, 助教 (20596605)
森 禎章 大阪医科大学, 医学部, 准教授 (70268192)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Keywords | 内リンパ腔電位 / クローディン / タイト結合 / 血管条辺縁細胞 / Ca2+濃度 / 蝸牛内リンパ腔電位 / クロディーン / Ca2+ / 末梢性感音難聴 / ノックアウトマウス / 急性虚血 / キメラマウス / Ca^<2+> / 相同組み換え / ES細胞 |
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| Research Abstract |
It had been reported that mutatios in the gene encoding claudin-14 cause deafness and nephrolithiasis. In this experiment, we made the claudin-14 knockout mice to examine the functional role of tight junctions in the endolyphatic surface cells. The obtained experimental results were as follows; 1) endocochlear potential (EP) in the endolymph in knockout mice was lower than that in wild type mice. In the preset, we are trying to measure the H+, K+, Na+,Ca2+ concentrations in endolymph. All above experimental results will be published in the next year.
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