Analysis of vascular remodeling by t-PA/t-PAR system

• Project/Area Number: 22590219
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General physiology
• Research Institution: Kinki University
• Principal Investigator: MATSUO Osamu 近畿大学, 医学部, 顧問 (40030879)
• Co-Investigator(Kenkyū-buntansha): UESHIMA Shigeru 近畿大学, 農学部, 教授 (30193791) ; OKADA Kiyotaka 近畿大学, 医学部, 講師 (20185432) ; KAWAO Naoyuki 近畿大学, 医学部, 助教 (70388510)
• Co-Investigator(Renkei-kenkyūsha): NAGAI Nobuyuki 長浜バイオ大学, アニマルバイオサイエンス学科, 教授 (90260281)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2012: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 血液凝固 / 血液レオロジー / t-PA受容体 / t-PA / 血管内皮細胞 / 組織修復 / 血管 / 蛋白分解活性 / 線溶系 / 血管平滑筋細胞 / t-PAR / ES細胞

Research Abstract

t-PAR-transfected endothelial cells enhanced the t-PA binding ability, t-PA-mediated plasminogen activation, migration and invasion of cells, and tube formation as compared with control endothelial cells. Vascular smooth muscle cells bound to t-PA and enhanced t-PA-mediated plasminogen activation. Therefore, it is thought that t-PA/t-PAR system on the membrane surface of these cells plays an important role on the tissue regeneration process.

Research Products

• [Journal Article] A smoll molecule inhibitor to plasminogen activator inhibitor 1 inhibits macrophage migration. (2013)
  • Author(s): Ichimura A, Matsumoto S, Suzuki S, Dan T, Yamaki S, Sato Y, Kiyomoto H, Ishii N, Okada K, Matsuo O, Hou FF, Vaughan DE, van Ypersele de Strihou S, Miyata T.
  • Journal Title: Arterioscler Thromb Vasc Biol. Volume: 33 Issue: 5 Pages: 935-942
  • DOI: 10.1161/atvbaha.113.301224
  • Peer Reviewed
• [Journal Article] Urokinase-type plasminogen activator and plasminogen mediate activation of macrophage phagocytosis during liver repair in vivo. (2012)
  • Author(s): Kawao N, Nagai N, Tamura Y, Horiuchi Y, Okumoto K, Okada K, Suzuki Y, Umemura K, Yano M, Ueshima S, Kaji H, Matsuo O.
  • Journal Title: Thromb Haemost Volume: 107 Issue: 04 Pages: 749-59
  • DOI: 10.1160/th11-08-0567
  • Peer Reviewed
• [Journal Article] A synthetic peptide derived from staphylokinase enhances plasminogen activator by tissue-type plasminogen activator. (2011)
  • Author(s): Okada K, Ueshima S, Matsuno H, Nagai N, Kawao N, Tanaka M, Matsuo O.
  • Journal Title: J Thromb Haemost Volume: 9 Issue: 5 Pages: 997-1006
  • DOI: 10.1111/j.1538-7836.2011.04257.x
  • Peer Reviewed
• [Journal Article] Plasminogen/plasmin modulates bone metabolism by regulating the osteoblast and osteoclast function (2011)
  • Author(s): Kanno Y, Ishisaki A, Nakajima K, Nishihara T, Toyoshima T, Okada K, Ueshima S, Matsushita K, Matsuo O
  • Journal Title: J Biol Chem Volume: 286 Issue: 11 Pages: 8952-8960
  • DOI: 10.1074/jbc.m110.152181
  • Peer Reviewed
• [Journal Article] The absence of urokinase type plasminogen activator receptor plays a role in the insulin -independent glucose metabolism. (2011)
  • Author(s): Tomogane K, Kanno Y, Kawashita E, Okada K, Takeuchi K, Ueshima S, Matsuo O, Matsuno H.
  • Journal Title: J Cardiovascu Pharmaco Volume: 57 Pages: 334-339
  • Peer Reviewed
• [Journal Article] A new synthetic peptide (SP) enhances plasminogen activation by t-PA (2011)
  • Author(s): Okada K.
  • Journal Title: J Thromb Haemost Volume: 9 Pages: 997-1006
  • Peer Reviewed
• [Journal Article] Urokinase-type plasminogen activator contributes to heterogeneity of macrophages at the border of damaged site during liver repair in mice. (2010)
  • Author(s): Kawao N, Nagai N, Tamura Y, Okada K, Yano M, Suzuki Y, Umemura K, Ueshima S, Matsuo O.
  • Journal Title: Thromb Haemost Volume: 105 Issue: 05 Pages: 892-900
  • DOI: 10.1160/th10-08-0516
  • Peer Reviewed
• [Journal Article] Enhancement o nno ytic activity in vascular endothelial cells by heterologous expression of adenine nucleotide translocase-1. (2010)
  • Author(s): Chikako Ishida
  • Journal Title: Blood Coagulation and Fibrinolysis Volume: 21 Pages: 272-278
  • Peer Reviewed
• [Presentation] The mechanism of enhancement on plasminogen activation and thrombolysis by synthetic nonadecapeptide. (2013)
  • Author(s): Okada K, Ueshima S, Kawao O, Tamura Y, Yano M, Matsuo O, Kaji H.
  • Organizer: The 90th Annual Meeting of the Physiology Society of Japan.
  • Place of Presentation: Tokyo
• [Presentation] 骨修復におけるプラスミノゲンの重要性について (2013)
  • Author(s): 河尾直之、田村行識,奥本勝美,矢野昌人,岡田清孝,松尾 理,梶 博史
  • Organizer: 第12回日本再生医療学会総会
  • Place of Presentation: 横浜
• [Presentation] The mechanism of enhancement on plasminogen activation and thrombolysis by synthetic nonadecapeptide. (2013)
  • Author(s): Okada K, Ueshima S, Matsuno H, Kawao N, Tamura Y, Yano M, Nagai N, Matsuo O
  • Organizer: 第90回日本生理学会大会
  • Place of Presentation: タワーホール船堀 (東京)
• [Presentation] 肝細胞周囲での線溶系酵素/受容体系による肝再生制御機構 (2012)
  • Author(s): 岡田清孝、上嶋 繁、河尾直之、田村行識、矢野昌人、梶 博史、松尾 理
  • Organizer: 第11回日本再生医療学会総会
  • Place of Presentation: 横浜
• [Presentation] 新規合成ペプチドとプラスミノーゲンの相互作用 (2012)
  • Author(s): 岡田清孝、上嶋 繁、河尾直之、田村行識、矢野昌人、梶 博史、松尾 理
  • Organizer: 第34回日本血栓止血学会学術集会
  • Place of Presentation: 東京
• [Presentation] 肝修復過程におけるu-PAによるマクロファージの機能制御 (2012)
  • Author(s): 河尾直之、永井信夫,田村行識,堀内喜高,奥本勝美,岡田清孝,矢野昌人,鈴木康裕,梅村和夫,梶 博史,松尾 理
  • Organizer: 第34回日本血栓止血学会学術集会
  • Place of Presentation: 東京
• [Presentation] Roles of urokinase-type plasminogen activator and plasminogen in both accumulation of macrophages and induction of their phenotype heterogeneity during liver repair. (2012)
  • Author(s): Kawao N, Nagai N, Tamura Y, Okumoto K, Okada K, Yano M, Suzuki Y, Umemura K, Kaji H, Matsuo O.
  • Organizer: The 89th Annual Meeting of the Physiology Society of Japan.
  • Place of Presentation: Matsumoto
• [Presentation] 脂肪細胞と血管平滑筋細胞の共存下におけるアントシアニンの効果 (2011)
  • Author(s): 上嶋繁
  • Organizer: 第21回日本病態生理学会
  • Place of Presentation: 東京
  • Year and Date: 2011-08-20
• [Presentation] Impaired accumulation of macrophages and absence of their phenotype heterogeneity during liver repair in urokinase-deficient mice. (2011)
  • Author(s): Kawao N, Nagai N, Tamura Y, Okumoto K, Okada K, Yano M, Suzuki Y, Umemura K, Ueshima S, Matsuo O.
  • Organizer: XXIII Congress of the International Society on Thrombosis and Haemostasis.
  • Place of Presentation: Kyoto
• [Presentation] Syntetic nonadecapeptide (SAK22-40) enhances plasminogen activation and thrombolysis. (2010)
  • Author(s): Okada K, Ueshima S, Matsuno H, Kawao N, Nagai N, Matsuo O.
  • Organizer: 20th International Congress on Fibrinolysis and Proteolysis
  • Place of Presentation: Amsterdam/ Nederland
• [Presentation] 肝細胞上での蛋白分解カスケード増幅系による肝再生調節機構の解析 (2010)
  • Author(s): 岡田清孝、上嶋 繁、河尾直之、永井信夫、松尾 理
  • Organizer: 第31回日本炎症・再生学会
  • Place of Presentation: 東京