| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Research Abstract |
Circadian clock are found in most of the peripheral tissues of animals. However, the physiological roles of the clocks in peripheral tissues are yet to be shown. We hypothesized that ovarian circadian clock regulates the responsiveness of ovarian cells to LH and play a role in controlling the timing of ovulation. First, we expand our previous experiment to show the role of LH in resetting phase of circadian clock in vitro to in vivo, and obtained the consistent results. Next, molecular biological methods were employed to evaluate the hypothesis in rat ovarian granulose cells. However, it was difficult to collect sufficient cells. Therefore, cell lines established from ovarian granulose cells were used in further experiments.
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