Study on the roles of DEC1 and DEC2 in the rhythmic regulation of lipid metabolism.

• Project/Area Number: 22590223
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Environmental physiology (including Physical medicine and Nutritional physiology)
• Research Institution: Hiroshima University
• Principal Investigator: NOSHIRO Mitsuhide 広島大学, 大学院・医歯薬保健学研究院, 准教授 (00144858)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 概日リズム / 時計遺伝子 / 脂質代謝 / 脂肪分化 / 核内受容体 / DEC1 / DEC2 / 遺伝子発現

Research Abstract

DEC1 and DEC2 are involved in various biological phenomena including clock systems, cell differentiation, and metabolism. I identified novel nuclear receptor response elements, ROR-response elements, in Dec1 and Dec2promoters. I also examined expression of Rorα Dec1, andDec2in differentiating 3T3-L1 adipocytes. The results suggested that RORαsuppresses adipogenicdifferentiation at a later stage of differentiation by RORE-mediated stimulation of Dec1and Dec2expression.

Research Products

• [Journal Article] Regulation of basic helix-loop-helix transcription factors Dec1 and Dec2 by RORα and their roles in adipogenesis. (2012)
  • Author(s): Ozaki N
  • Journal Title: Genes to Cells Volume: 17 Issue: 2 Pages: 107-121
  • DOI: 10.1111/j.1365-2443.2011.01574.x
  • Peer Reviewed
• [Journal Article] BHLH transcription factor DEC2 regulates pro-apoptotic factor Bim in human oral cancer HSC-3 cells (2012)
  • Author(s): Wu, Y., Sato, F., Kawamoto, T., Noshiro, M.
  • Journal Title: Biomed Res Volume: 33 Pages: 75-82
  • NAID: 130004470811
  • URL: http://www.ncbi.nlm.nih.gov/pubmed/22572381
  • Peer Reviewed
• [Journal Article] Identification of a new clock-related element EL-box involved in circadian regulation by BMAL1/CLOCK and HES1 (2012)
  • Author(s): Ueshima, T., Kawamoto, T., Honda, K.K, Noshiro, M., Fujimoto, K., Nakao, S., Ichinose, N., Hashimoto, S., Gotoh, O., Kato, Y
  • Journal Title: Gene Volume: 510 (2) Issue: 2 Pages: 118-125
  • DOI: 10.1016/j.gene.2012.08.022
  • Peer Reviewed
• [Journal Article] Smad3 and Snail show circadian expression in human gingival fibroblasts, human mesenchymal stem cell, and in mouse liver (2012)
  • Author(s): Sato, F., Wu, Y., Noshiro, M.
  • Journal Title: Biochem Biophys Res Commun Volume: 419 Issue: 2 Pages: 441-446
  • DOI: 10.1016/j.bbrc.2012.02.076
  • Peer Reviewed
• [Journal Article] The basic helix-loop-helix transcription factor DEC2 inhibits TGF-beta-induced tumor progression in human pancreatic cancer BxPC-3 cells (2012)
  • Author(s): Sato, F., Kawamura, H., Kawamoto, T., Noshiro, M.,(他10名)
  • Journal Title: Int J Mol Med Volume: 30 Issue: 3 Pages: 495-501
  • DOI: 10.3892/ijmm.2012.1037
  • Peer Reviewed
• [Journal Article] The BHLH transcription factor DEC1 plays an important role in the epithelial-mesenchymal transition of pancreatic cancer (2012)
  • Author(s): Wu, Y., Kawamoto, T., Noshiro, M., et al.(他10名)
  • Journal Title: Int J Oncol Volume: 41 Issue: 4 Pages: 1337-1346
  • DOI: 10.3892/ijo.2012.1559
  • Peer Reviewed
• [Journal Article] Basic helix-loop-helix transcription factors DEC1 and DEC2 regulate the paclitaxel-induced apoptotic pathway of MCF-7 human breast cancer cells (2011)
  • Author(s): Wu, Y., Sato, F., Kawamoto, T., Fujimoto, K., Noshiro, M., (他4名)
  • Journal Title: Int J Mol Med Volume: 27 Issue: 4 Pages: 491-495
  • DOI: 10.3892/ijmm.2011.617
  • Peer Reviewed
• [Journal Article] Roles of DEC1 and DEC2 in the core loop of the circadian clock, and clock outputs to metabolism. (2011)
  • Author(s): Kato Y, Noshiro M, Fujimoto K, Kawamoto T.
  • Journal Title: Hirosaki Med.J. Volume: 61(Suppl.)
• [Journal Article] Basic helix-loop-helix transcription factors DEC1 and DEC2 regulate the paclitaxel-induced apoptotic pathway of MCF-7 human breast cancer cells. (2011)
  • Author(s): Wu, Y., Sato, F., Noshiro, M., Kato, Y., Kijima, H., et al.
  • Journal Title: Int J Mol Med Volume: 27 Pages: 491-495
  • Peer Reviewed
• [Journal Article] Anti-apoptotic effect of the basic helix-loop-helix (bHLH) transcription factor DEC2 in human breast cancer cells (2010)
  • Author(s): Liu, Y., Sato, F., Kawamoto, T., Noshiro, M.
  • Journal Title: Genes Cells Volume: 15 Issue: 4 Pages: 315-325
  • DOI: 10.1111/j.1365-2443.2010.01381.x
  • Peer Reviewed
• [Journal Article] Anti-apoptotic effect of the basic helix-loop-helix(bHLH) transcription factor DEC2 in human breast cancer cells. (2010)
  • Author(s): Liu, Y., Sato, F., Noshiro, M., Kato, Y., Kijima, H., et al.
  • Journal Title: Genes to Cells Volume: 15 Pages: 315-325
  • Peer Reviewed
• [Presentation] DEC 研究の展望 (2012)
  • Author(s): 血圧制御など 加藤幸夫 シンポジウム
  • Organizer: 時刻と時間の生理学
  • Place of Presentation: 札幌
  • Year and Date: 2012-03-10
• [Presentation] 時計遺伝子 DEC1 の発現調節と機能における核内受容体との関連 (2012)
  • Author(s): 能城光秀
  • Organizer: 時刻と時間の生理学
  • Place of Presentation: 札幌
  • Year and Date: 2012-03-10
• [Presentation] 概日リズムを制御する新規時計エレメント EL-box の同定 (2012)
  • Author(s): 河本健、能城光秀、藤本勝巳、加藤幸夫
  • Organizer: 第54回歯科基礎医学会学術大会・総会
  • Place of Presentation: 郡山
• [Presentation] 概日リズムを制御する新規時計エレメントEL-boxの同定 (2012)
  • Author(s): 河本健
  • Organizer: 第54回歯科基礎医学会学術大会・総会
  • Place of Presentation: 郡山
• [Presentation] 核内受容体 RORαによる時計遺伝子DEC1,DEC2 の発現調節と脂肪分化: 尾崎徳継 (2011)
  • Author(s): 能城光秀、河本健、福崎麗、藤本勝巳、丹根一夫、加藤幸夫
  • Organizer: 第52回日本生化学会中国・四国支部例会
  • Place of Presentation: 広島市
• [Presentation] 間葉系幹細胞の軟骨分化におけるDEC2の役割 (2011)
  • Author(s): 笹本智子、藤本勝巳、金輪真佐美、河本健、能城光秀、道田将彦、尾崎徳継、丹根一夫、加藤幸夫
  • Organizer: 第52回日本生化学会中国・四国支部例会
  • Place of Presentation: 広島市
• [Presentation] 間葉系幹細胞の増殖・分化ならびにインテクリン発現に対する転写因子GATA6の作用 (2011)
  • Author(s): 道田将彦、河本健、能城光秀、加藤幸夫, ら
  • Organizer: 第52回日本生化学会中国・四国支部例会
  • Place of Presentation: 広島市
• [Presentation] 核内受容体RORαによる時計遺伝子DEC1,DEC2の発現調節と脂肪分化 (2011)
  • Author(s): 尾崎徳継、能城光秀、加藤幸夫, ら
  • Organizer: 第52回日本生化学会中国・四国支部例会
  • Place of Presentation: 広島市
• [Presentation] 核内受容体(RORα)とSREBP1cによるベーシック・ヘリックス・ループ・ヘリックス型転写因子(Dec1, Dec2)の発現調節と脂肪分化 (2010)
  • Author(s): 尾崎徳継, 能城光秀, 福崎麗, 河本健, 藤本勝巳, 丹根一夫, 加藤幸夫
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会大会・合同大会
  • Place of Presentation: 神戸市
  • Year and Date: 2010-12-09
• [Presentation] 核内受容体(RORα)による時計遺伝子(Dec1, Dec2)の制御 (2010)
  • Author(s): 尾崎徳継、能城光秀、河本健、藤本勝巳、丹根一夫、加藤幸夫
  • Organizer: 第51回日本生化学会中国・四国支部例会
  • Place of Presentation: 山口市
  • Year and Date: 2010-05-14
• [Presentation] 核内受容体 (ROR・) による時計遺伝子 (Dec1, Dec2) の制御 (2010)
  • Author(s): 尾崎徳継、能城光秀、河本健、藤本勝巳、丹根一夫、加藤幸夫
  • Organizer: 第51回日本生化学会中国・四国支部例会
  • Place of Presentation: 山口市
• [Presentation] 核内受容体 (ROR・) と SREBP1c によるベーシック・ヘリックス・ループ・ヘリックス型転写因子(Dec1, Dec2) の発現調節と脂肪分化 (2010)
  • Author(s): 尾崎徳継,能城光秀,福崎麗, 河本健,藤本勝巳,丹根一夫,加藤幸夫
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会大会・合同大会
  • Place of Presentation: 神戸市
• [Patent(Industrial Property Rights)] 脂質代謝関連疾患の検査方法、ならびに脂質代謝関連疾患の予防および/または治療剤の評価方法 (2011)
  • Inventor(s): 加藤幸夫、河本健、藤本勝巳、能城光秀
  • Industrial Property Rights Holder: 広島大学
  • Industrial Property Number: 2011-022172
  • Filing Date: 2011-02-03
• [Patent(Industrial Property Rights)] 非ヒト動物、細胞、血圧調節物質の評価方法、血圧調節条件の評価方法および血圧の調節方 (2010)
  • Inventor(s): 加藤幸夫、河本健、能城光秀、中島歩
  • Industrial Property Rights Holder: 広島大学
  • Industrial Property Number: 2010-236891
  • Filing Date: 2010-10-21
• [Patent(Industrial Property Rights)] 非ヒト動物、細胞、血圧調節物質の評価方法、血圧調節条件の評価方法および血圧の調節方法 (2010)
  • Inventor(s): 加藤幸夫、河本健、能城光秀、中島歩
  • Industrial Property Rights Holder: 広島大学
  • Industrial Property Number: 2010-236891
  • Filing Date: 2010-10-21