Study of regeneration treatment with cobalt chloride-induced retinal degeneration model

• Project/Area Number: 22590239
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General pharmacology
• Research Institution: Gifu University
• Principal Investigator: NIWA Masayuki 岐阜大学, 医学部, 教授 (40156146)
• Co-Investigator(Kenkyū-buntansha): HARA Akira 岐阜大学, 大学院・医学系研究科, 教授 (10242728)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 再生医学 / 中枢神経 / 網膜 / 神経障害 / ES細胞 / コバルトクロライド / NMDA / 奇形種発現 / ＥＳ細胞 / 再生 / 奇形種 / 網膜障害 / 再生治療

Research Abstract

The ultimate goal of this study is treatment of retinal degeneration by regeneration. As a pre-stage, we constructed suitable regeneration condition using retinal degeneration model treating with CoCl2or NMDA. The results indicate hypoxia condition might be good for neuronal differentiation, and herpes simplex virus-thymidine kinase transgene transfected ES cells is good for prevention of expression of teratoma.

Research Products

• [Journal Article] Keratinocyte stem cells but not melanocyte stem cells are the primary target for radiation- induced hair graying. (2013)
  • Author(s): Aoki H, Hara A
  • Journal Title: J Invest Dermatol Volume: 155巻(in press)
  • Peer Reviewed
• [Journal Article] Keratinocyte stem cells but not melanocyte stem cells are the primary target for radiation-induced hair graying. (2013)
  • Author(s): Aoki H
  • Journal Title: J Invest Dermatol Volume: 155 Pages: 600-10
  • DOI: 10.1172/jci63572
  • Peer Reviewed
• [Journal Article] Genetic ablation of Rest leads to in vitro-specific derepression of neuronal genes during neurogenesis. (2012)
  • Author(s): Aoki H, Hara A
  • Journal Title: Devel opment Volume: 139巻 Pages: 667-677
  • Peer Reviewed
• [Journal Article] PBN fails to suppress in delayed neuronal death of hippocampal CA1 injury following transient forebrain ischemia in gerbils. (2012)
  • Author(s): Goda W, Hara A, Niwa M
  • Journal Title: Neurosci Lett Volume: 517巻 Pages: 47-51
  • Peer Reviewed
• [Journal Article] PBN fails to suppress in delayed neuronal death of hippocampal CA1 injury following transient forebrain ischemia in gerbils. (2012)
  • Author(s): Goda W
  • Journal Title: Neurosci Lett Volume: 517 Issue: 1 Pages: 47-51
  • DOI: 10.1016/j.neulet.2012.04.016
  • Peer Reviewed
• [Journal Article] Genetic ablation of Rest leads to in vitro-specific derepression of neuronal genes during neurogenesis (2012)
  • Author(s): Aoki H, Hara A, et al
  • Journal Title: Development Volume: 139 Issue: 4 Pages: 667-677
  • DOI: 10.1242/dev.072272
  • Peer Reviewed
• [Journal Article] Functionally distinct melanocytes populations revealed by reconstitution of hair follicles in mice. (2011)
  • Author(s): Aoki H, Hara A
  • Journal Title: Pigment Cell Melanoma Res Volume: 24巻 Pages: 125-135
  • Peer Reviewed
• [Journal Article] Protective Effect of Kit Signaling for Melanocyte Stem Cells against Radiation-Induced Genotoxic Stress. (2011)
  • Author(s): Aoki H, Hara A
  • Journal Title: J Invest Dermatol. Volume: 131巻 Pages: 1906-1915
  • Peer Reviewed
• [Journal Article] Increase of galectin-3 expression in microglia by hyperthermia in delayed neuronal death of hippocampal CA1 following transient forebrain ischemia . (2011)
  • Author(s): Satoh K, Niwa M, Hara A
  • Journal Title: Neurosci Lett. Volume: 504巻 Pages: 199-203
  • Peer Reviewed
• [Journal Article] Galectin-3 expression in delayed neuronal death of hippocampal CA1 following transient forebrain ischemia, and its inhibition by hypothermia. (2011)
  • Author(s): Satoh K, Niwa M, Hara A
  • Journal Title: Brain Res. Volume: 1382巻 Pages: 266-274
  • Peer Reviewed
• [Journal Article] Increase of galectin-3 expression in microglia by hyperthermia in delayed neuronal death of hippocampal CA1 following transient forebrain ischemia (2011)
  • Author(s): Satoh K, Niwa M, et al
  • Journal Title: Neurosci Lett Volume: 504 Issue: 3 Pages: 199-203
  • DOI: 10.1016/j.neulet.2011.09.015
  • Peer Reviewed
• [Journal Article] Galectin-3 expression in delayed neuronal death of hippocampal CA1 following transient forebrain ischemia, and its inhibition by hypothermia (2011)
  • Author(s): Satoh K, Niwa M, et al
  • Journal Title: Brain Res Volume: 1382 Pages: 266-274
  • DOI: 10.1016/j.brainres.2011.01.049
  • Peer Reviewed
• [Journal Article] Protective Effect of Kit Signaling for Melanocyte Stem Cells against Radiation-Induced Genotoxic Stress (2011)
  • Author(s): Aoki H, Hara A, et al
  • Journal Title: J Invest Dermatol Volume: 131 Issue: 9 Pages: 1906-1915
  • DOI: 10.1038/jid.2011.148
  • Peer Reviewed
• [Journal Article] Functionally distinct melanocytes populations revealed by reconstitution of hair follicles in mice (2011)
  • Author(s): Aoki H, Hara A, et al
  • Journal Title: Pigment Cell Melanoma Res Volume: 24 Issue: 1 Pages: 125-135
  • DOI: 10.1111/j.1755-148x.2010.00801.x
  • Peer Reviewed
• [Journal Article] Folate antagonist, methotrexate induces neuronal differentiation of human embryonic stem cells transplanted into nude mouse retina. (2010)
  • Author(s): Hara A, Niwa M
  • Journal Title: Neurosci Lett. Volume: 477巻 Pages: 138-143
  • Peer Reviewed
• [Journal Article] Folate antagonist, methotrexate induces neuronal differentiation of human embryonic stem cells transplanted into nude mouse retina. (2010)
  • Author(s): Hara A, Niwa M, ら
  • Journal Title: Neurosci Lett. Volume: 477 Pages: 138-143
  • Peer Reviewed
• [Presentation] 低体温および高体温は一過性前脳虚血後の遅発性神経細胞死および Galectin-3 発現に影響を及ぼす (2013)
  • Author(s): 佐藤国夫、原 明、丹羽雅之
  • Organizer: 第86回日本薬理学会年会
  • Place of Presentation: 博多
  • Year and Date: 2013-03-23
• [Presentation] 低体温および高体温は一過性前脳虚血後の遅発性神経細胞死およびGalectin-3発現に影響を及ぼす (2013)
  • Author(s): 佐藤国夫
  • Organizer: 第８６回日本薬理学会年会
  • Place of Presentation: 博多 福岡国際会議場
• [Presentation] Galectin-3 contributes to delayed neuronal death of hippocampal CA1 following transient forebrain ischemia (2011)
  • Author(s): Niwa M, Satoh K, Hara A
  • Organizer: 10th European Meeting on Glial Cells in Health and Disease
  • Place of Presentation: Praque, Czech
• [Book] Human embryonic stem cells transplanted into mouse retina induces neural differentiation.. In: Tumors of the central nervous system. Springer, Stem Cells and Cancer Stem Cells, 2 巻, Part 4 (2012)
  • Author(s): Hara A, Niwa M
  • Publisher: Springer
• [Book] Hypothermia and Hyperthermia affect neuronal degeneration, delayed neuronal death and microglial activation following transient forebrain ischemi. In. Hypothermia: Prevention, Recognition and Treatment: ed Delgado JIV and Garza VGF (2012)
  • Author(s): Niwa M, Hara A
  • Publisher: Nova Science Publish
• [Book] Human embryonic stem cells transplanted into mouse retina induces neural differentiation (2012)
  • Author(s): Hara A, Niwa M, et al
  • Publisher: In : Stem Cells and Cancer Stem Cells, Volume 2, ed by Hayat MA, Springer(In press)
• [Book] Hypothermia and Hyperthermia affect neuronal degeneration, delayed neuronal death and microglial activation following transient forebrain ischemi (2012)
  • Author(s): Niwa M, Hara A, et al
  • Publisher: In. Hypothermia : Prevention, Recognition and Treatment : ed Delgado JIV and Garza VGF, Nova Science Publish(In press)
• [Book] Human embryonic stem cells transplanted into mouse retina induces neural differentiation. In : Tumors of the central nervous system (2011)
  • Author(s): Hara A, Niwa M, ら
  • Publisher: Springer