Elucidation of cellular network for plastic change of vascular smooth muscle cell.

• Project/Area Number: 22590240
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General pharmacology
• Research Institution: Mie University
• Principal Investigator: OKAGAKI Tsuyoshi 三重大学, 生物資源学研究科, 教授 (80185412)
• Co-Investigator(Kenkyū-buntansha): OOI Atsushi 三重大学, 大学院生物資源学研究科, 准教 (70203693) ; NAKAMURA Akio 群馬大学, 大学院医学系研究科, 講師 (30282388)
• Project Period (FY): 2010-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 血管平滑筋 / 動脈硬化症 / 細胞骨格 / シグナル伝達 / 形質変換 / 血管 / 薬理学 / 遺伝子 / タンパク質 / 細胞・組織 / 蛋白質

Research Abstract

Vascular smooth muscle cell composing wall of blood vessel contributes the control of blood pressure, by stretching or contracting the cell body. Life style related illness is often originated by atherosclerosis. In such vessel, normal contractile smooth muscle cell changes its property and differentiates into synthetic phenotype. To know the mechanism of the phenotypic change of the cell, we investigated expression level of several genes, and we found that several genes including T-plastin were related to the beginning of atherosclerosis.

Research Products

• [Journal Article] Interaction of peptide-bound beads with lipopolysaccharide and lipoproteins (2014)
  • Author(s): Suzuki MM, Matsumoto M, Omi H, Kobayashi T, Nakamura A, Kishi H, Kobayashi S, Takagi T.
  • Journal Title: J. Microbiol. Methods. Volume: 100 Pages: 137-141
  • Peer Reviewed
• [Journal Article] 炭酸ナトリウム添加による凍結ゴマサバ肉の加熱ゲル形成能の向上 (2013)
  • Author(s): 美濃松兼、矢野竹男、三島隆、青木恭彦、大井淳史
  • Journal Title: 日本食品工学会誌 Volume: 14 Pages: 29-36
  • NAID: 130005078812
  • Peer Reviewed
• [Journal Article] Calcium-dependent regulation of the motor activity of recombinant full-length Physarum myosin (2012)
  • Author(s): Zhang Y1, Kawamichi H, Tanaka H, Yoshiyama S, Kohama K, Nakamura A
  • Journal Title: J. Biochem. Volume: 152 Pages: 185-190
  • NAID: 10031166127
  • Peer Reviewed
• [Journal Article] Down-regulation of myosin light chain kinase expression in vascular smooth muscle cells accelerates cell proliferation : requirement of its actin-binding domain for reversion to normal rates (2012)
  • Author(s): Wang HH, Nakamura A, Yoshiyama S, Ishikawa R, Cai N, Ye LH, Takano-Ohmuro H, Kohama K.
  • Journal Title: J. Pharmacol. Sci. Volume: 119 Pages: 91-96
  • NAID: 10030760488
  • Peer Reviewed
• [Journal Article] Effect of cigarette smoke components on vascular smooth muscle cell migration toward platelet-derived growth factor BB (2011)
  • Author(s): Yoshiyama S, Horinouchi T, Miwa S, Wang HH, Kohama K, Nakamura A.
  • Journal Title: J. Pharmacol Sci. Volume: 15 Pages: 532-535
  • NAID: 10029893589
  • Peer Reviewed
• [Journal Article] 平滑筋からのmyosinおよび調節タンパク質の精製法の改良 (2011)
  • Author(s): 近藤哲也、鈴木亮、岡垣壮
  • Journal Title: 三重大学大学院生物資源学研究科紀要 Volume: 第37号 Pages: 87-97
  • NAID: 40018949003
  • Peer Reviewed
• [Journal Article] Thermal stability of carp actin in its polymerized form (2011)
  • Author(s): Ooi, A., Okagaki, T.
  • Journal Title: Fish. Sci. Volume: 77 Pages: 1053-1059
  • NAID: 10029654910
  • Peer Reviewed
• [Journal Article] Thermal stability of carp actin in its polymerized form (2011)
  • Author(s): Ooi, A., Okagaki, T.
  • Journal Title: Fish.Sci. Volume: 77 Pages: 1053-1059
  • NAID: 10029654910
  • Peer Reviewed
• [Journal Article] 平滑筋ミオシンおよび調節タンパク質の精製法の改良 (2011)
  • Author(s): 近藤哲也、鈴木亮、岡垣壮
  • Journal Title: 三重大学生物資源学研究科紀要 Volume: 37 Pages: 87-97
  • Peer Reviewed
• [Journal Article] Calcium regulation of the ATPase activity of Physarum and scallop myosins using hybrid smooth muscle myosin : the role of the essential light chain (2010)
  • Author(s): Zhang Y, Nakamura A, Kawamichi H, Yoshiyama S, Katayama T, Kohama K.
  • Journal Title: FEBS Lett. Volume: 584 Pages: 3486-3491
  • Peer Reviewed
• [Journal Article] マダイミオシン加熱ゲル形成に及ぼすグルコン酸塩の影響 (2010)
  • Author(s): 大井敦史、田村陽二郎、岡垣壮
  • Journal Title: 日本水産学会誌 Volume: 76 Pages: 1066-1072
  • Peer Reviewed
• [Journal Article] Calcium regulation of ATPase activity of Physarum and scallop myosin using hybrid smooth muscle myosin : the role of the essential light chain. (2010)
  • Author(s): Zhang, Nakamura, Kawamichi, Yoshiyama, Katayama, Kohama
  • Journal Title: FEBS lett. Volume: 584 Pages: 3486-3491
  • Peer Reviewed
• [Presentation] コイ筋原線維ATPase活性のCa-感受性に対するCタンパク質の効果 (2013)
  • Author(s): 岡垣壮、西村陽平、磯西啓太、別府はるか、戸澤彩香、大井淳史
  • Organizer: 平成25年度日本水産学会秋季大会
  • Place of Presentation: 三重大学(津市)
• [Presentation] 血管平滑筋ミオシン軽鎖キナーゼのアクチン連関による新しい機能 (2013)
  • Author(s): 中村彰夫、松本篤、王洪輝、謝策、吉山伸司、河原田律子、高顛、石川良樹、小濱一弘
  • Organizer: 第86回日本薬理学会年会
  • Place of Presentation: 福岡国際会議場(福岡市中央区)
• [Presentation] コイ筋原線維ATPase活性のCa-感受性に対するCタンパク質の効果 (2013)
  • Author(s): 岡垣壮、西村陽平、磯西啓太、別府はるか、戸澤彩香、大井淳史
  • Organizer: 日本水産学会秋季大会
  • Place of Presentation: 三重大学
• [Presentation] 冷凍ゴマサバ肉へのアルカリ直接添加による足の増強 (2012)
  • Author(s): 美濃松兼、矢野竹男、三島隆、青木恭彦、大井淳史
  • Organizer: 平成24年度日本水産学会秋季大会
  • Place of Presentation: 水産大学校(下関市)
• [Presentation] ニコチンの血管平滑筋細胞の遊走と遺伝子発現に対する影響 (2012)
  • Author(s): 吉山伸司、小濱一弘、中村彰夫
  • Organizer: 第85回日本薬理学会年会
  • Place of Presentation: 国立京都国際会館(京都市左京区)
• [Presentation] 冷凍ゴマサバ肉へのアルカリ直接添加による足の増強 (2012)
  • Author(s): 美濃松兼、矢野竹男、三島隆、青木恭彦、大井淳史
  • Organizer: 日本水産学会秋季大会
  • Place of Presentation: 水産大学校
• [Presentation] 蛍光消光法によるコイ骨格筋F-アクチンの構造学的解析 (2010)
  • Author(s): 大井敦史
  • Organizer: 平成22年度日本水産学会秋季大会
  • Place of Presentation: 京都大学(京都市左京区)
• [Presentation] 蛍光消光法によるコイ骨格筋F-アクチンの構造学的解析 (2010)
  • Author(s): 大井敦史
  • Organizer: 平成22年度日本水産学会秋季大会
  • Place of Presentation: 京都大学