| Project/Area Number |
22590241
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General pharmacology
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| Research Institution | The University of Tokushima |
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Principal Investigator |
TSUCHIYA Koichiro 徳島大学, 大学院・ヘルスバイオサイエンス研究部, 教授 (70301314)
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| Co-Investigator(Kenkyū-buntansha) |
TOMITA Shuhei 鳥取大学, 医学部, 教授 (00263898)
ISHIZAWA Keisuke 徳島大学, 大学院・ヘルスバイオサイエンス研究部, 助教 (60398013)
HAMANO Shuichi 徳島大学, 大学院・ヘルスバイオサイエンス研究部, 准教授 (60530392)
TAMAKI Toshiaki 徳島大学, 大学院・ヘルスバイオサイエンス研究部, 教授 (80179879)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 腎臓 / 亜硝酸 / 亜硝酸塩 / 一酸化窒素 / 代謝 / 腎保護作用 / 耐糖能 / 硝酸塩 / 糸球体内皮細胞 / ラット / AMPK / STZ / MIN6細胞 / 硝酸 / 経口 / 腎糸球体内皮細胞 / マウス / OGTT |
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| Research Abstract |
Nitric oxide (NO) plays multipotent effects in many kinds of physiological processes. NO is generated by NO synthase (NOS), and disorders in the NOS is known to be related to diabetic nephropathy. Therefore, it has been believed that the production of NO from nitrite by non-enzymatic or enzymatic reduction responsible for the renoprotective effects of it. However, in this study, we found that nitrite suppress intracellular ATP level, then activates 5’AMP-activated protein kinase(AMPK), and following endotherial NOS activation. The activation of AMPK-eNOS pathway in endotherial cells is supposed to play a significant renal protective role in the nitrite action in addition to the NOS-independent NO production by nitrite.
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