In vitro blood-brain barrier reconstruction model (BBB Kit) and their application to the functional analysis
| Project/Area Number |
22590243
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
General pharmacology
|
|---|
| Research Institution | Nagasaki University |
|---|
Principal Investigator |
NIWA Masami 長崎大学, 大学院・医歯薬学総合研究科, 教授 (20136641)
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
TANAKA Kunihiko 長崎大学, 大学院・医歯薬学総合研究科, 准教授 (80380955)
|
|---|
| Project Period (FY) |
2010 – 2012
|
| Project Status |
Completed (Fiscal Year 2012)
|
| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
|
|---|
| Keywords | 中枢 / 末梢神経 / 血液脳関門 / サル血液脳関門 / 血液脳関門再構成モデル / サル脳毛細血管内皮細胞 / 脳ペリサイト / アストロサイト / 薬物脳内移行性 / タイトジャンクション / ラット血液脳関門 |
|---|
| Research Abstract |
The blood-brain barrier (BBB), a member of the neurovascular unit (NVU), functions in communication between brain capillary endothelial cells and other neighboring cells such as astrocytes and pericytes. Many drugs that are currently under development are intended to act centrally, but they do not cross the BBB. Furthermore, the crosstalk between the BBB and related cells contributing to the NVU remains to be elucidated. Based on our breakthrough in using rat primary brain capillary endothelial cells, pericytes and astrocytes to create a triple co-culture BBB model (BBB Kit), an in vitro models for screening the BBB penetration of drug candidates and research on BBB pathophysiology, we succeeded in modeling the monkey BBB with primary monkey brain capillary endothelial cells, rat pericytes and rat astrocytes. In addition, we constructed a porcine BBB model consisting of porcine brain capillary endothelial cells, rat pericytes, and rat astrocytes
|
Report
(4 results)
Research Products
(25 results)
-
-
-
-
[Journal Article] Comparison of brain capillary endothelial cell-based and epithelial (MDCK-MDR1, Caco-2 and VB-Caco-2) cell-based surrogate blood-brain barrier penetration models2012
Author(s)
Eva Hellinger, Szilvia Veszelka, Andrea Toth, Fruzsina Walter, Agnes Kittel, Monika Laura Bakk, Karoly Tihanyi, Viktor Hada, Shinsuke Nakagawa, Thuy Dinh Ha Duy, Masami Niwa, Maria A Deli, Monika Vastag.
-
Journal Title
Eurpean Jounal of Pharmaceutics and Biopharmaceutocs
Volume: 82
Pages: 34-350
Related Report
-
-
-
-
-
-
[Journal Article] Pitavastatin strengthens the barrier integrity in primary cultures of rat brain endothelial cells2010
Author(s)
Yoichi Morofuji, Shinsuke Nakagawa, Gohei So, Takeshi Hiu, Shoji Horai, Kentaro Hayashi, Kunihiko Tanaka, Kazuhiko Suyama, Maria A. Deli, Isumi Nagata, Masami Niwa
-
Journal Title
Cellular and Molecular Neurobiology
Volume: 30
Pages: 727-735
NAID
Related Report
-
[Journal Article] Protection of the blood-brain barrier by pentosan against amyloid-β-induced toxicity2010
Author(s)
Maria A. Deli, Szilvia Veszelkaa, Boglarka Csiszara, Andrea Totha, Agnes Kittel, Maria Csetec, Aron Siposc, Aniko Szalaic, Livia Fulopd, Botond Penked, Csongor S. Abrahama, Masami Niwa.
-
Journal Title
Journal of Alzheimer's Disease
Volume: 22
Pages: 777-794
Related Report
-
-
-
-
-
-
-
-
-
-
-
[Presentation] An In vitro monkey BBB model.2010
Author(s)
SNakagawa, MA Deli1, DHD Thuy, M Sagara, N Yamada, G So, R Tatsumi, K Tanaka, M Niwa
Organizer
13th International Symposium on "Signalling at the Blood-Brain and Blood-Tumour Barriers
Place of Presentation
Zurich
Related Report
-
-
-
