Physiological role of calcium transport system regulated by phosphatidylinositol

• Project/Area Number: 22590254
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General pharmacology
• Research Institution: Fukuoka University
• Principal Investigator: KITA Satomi 福岡大学, 医学部, 講師 (10461500)
• Co-Investigator(Kenkyū-buntansha): YAMAMOTO Shintaro 福岡大学, 医学部, 准教授 (40336110)
• Co-Investigator(Renkei-kenkyūsha): IYODA Takuya 福岡大学, 医学部, 助教 (80465715)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2012: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2010: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: 脂質キナーゼ / 血管リモデリング / 脂質代謝異常 / シグナル伝達 / 薬理学

Research Abstract

To clarify the role of NCX1 and TRPC3 in cardiovascular diseases (hypertension, arteriosclerosis), we generated TRPC3-transgenic (Tg) mice by overexpressing the wild-type and dominant negative TRPC3 using the smooth muscle α-actin promoter. Using these mice, we showed that NCX1 and TRPC3 may predominantly play an important role in α 1-adrenoreceptor agonist-induced vascular responses and blood pressure regulation. Furthermore, we found that blood recovery after unilateral hindlimb ischemia was significantly augmented in NCX1+/- mice compared with that in wild-type mice, suggesting that NCX1 is involved in ischemia-induced angiogenesis.

Research Products

• [Journal Article] Preferential involvement of Na+/Ca2+ exchanger type-1 in the brain damage caused by transient focal cerebral ischemia in mice. (2012)
  • Author(s): Morimoto N, Kita S, Shimazawa M, Namimatsu H, Tsuruma K, Hayakawa K, Mishima K, Egashira N, Iyoda T, Horie I, Gotoh Y, Iwasaki K, Fujiwara M, Matsuda T, Baba A, Komuro I, Horie K, Takeda J, Iwamoto T, Hara H.
  • Journal Title: Biochem Biophys Res Commun Volume: 429(3-4) Issue: 3-4 Pages: 186-190
  • DOI: 10.1016/j.bbrc.2012.10.114
  • Peer Reviewed
• [Journal Article] Na+/Ca2+ exchanger 2-heterozygote knockout mice display decreased acetylcholine release and altered colonic motility in vivo. (2012)
  • Author(s): Azuma YT, Nishiyama K, Kita S, Komuro I, Nakajima H, Iwamoto T, Takeuchi T
  • Journal Title: Neurogastroenterol Motil Volume: 24(12) Issue: 12
  • DOI: 10.1111/nmo.12029
  • Peer Reviewed
• [Journal Article] Effect of heterozygous deletion of WNK1 on the WNK-OSR1/SPAK-NCC/NKCC1/NKCC2 signal cascade in the kidney and blood vessels. (2012)
  • Author(s): Susa K, Kita S, Iwamoto T, Yang SS, Lin SH, Ohta A, Sohara E, Rai T, Sasaki S, Alessi DR, Uchida S.
  • Journal Title: Clin Exp Nephrol Volume: 16(4) Issue: 4 Pages: 530-538
  • DOI: 10.1007/s10157-012-0590-x
  • NAID: 10031058026
  • Peer Reviewed
• [Journal Article] Functional interaction between Na+/Ca2+ exchanger and sodium-transporting proteins in plasma membrane microdomains. (2012)
  • Author(s): Kita, S.
  • Journal Title: Nihon Yakurigaku Zasshi Volume: 139 Pages: 61-65
  • NAID: 10030456141
• [Journal Article] Effect of heterozygous deletion of WNK1 on the WNK-OSR1/SPAK-NCC/NKCC1/NKCC2 signal cascade in the kidney and blood bessels (2012)
  • Author(s): Susa, K.
  • Journal Title: Clin Exp Nephrol Volume: (in press)
  • Peer Reviewed
• [Journal Article] Functional interaction between Na^+/Ca^<2+> exchanger and sodium-transporting protein in plasma membrane microdomains (2012)
  • Author(s): Kita, S.
  • Journal Title: Nihon Yakurigaku Zasshi Volume: 139 Pages: 61-65
  • NAID: 10030456141
  • Peer Reviewed
• [Journal Article] TRIC-A channels in vascular smooth muscle contribute to blood pressure maintenance (2011)
  • Author(s): Yamazaki D, Tabara Y, Kita S, Hanada H, Komazaki S, Naitou D, Mishima A, Nishi M, Yamamura H, Yamamoto S, Kakizawa S, Miyachi H, Yamamoto S, Miyata T, Kawano Y, Kamide K, Ogihara T, Hata A, Umemura S, Soma M, Takahashi N, Imaizumi Y, Miki T, Iwamoto T, Ta
  • Journal Title: Cell Metab Volume: 14 Issue: 2 Pages: 231
  • DOI: 10.1016/j.cmet.2011.05.011
  • NAID: 120003255678
  • URL: http://hdl.handle.net/2433/143634
  • Peer Reviewed
• [Journal Article] Protein kinase A-mediated phosphorylation of TRPC6 channels underlies suppression of angiotensin II-induced vasoconstriction (2011)
  • Author(s): Nishioka K, Nishida M, Ariyoshi M, Saiki S, Jian Z, Hirano M, Nakaya M, Sato Y, Kita S, Iwamoto T, Hirano K, Inoue R, Kurose H
  • Journal Title: Arterioscler.Thromb.Vasc.Biol. Volume: 31 Issue: 10 Pages: 2278-2286
  • DOI: 10.1161/atvbaha.110.221010
  • Peer Reviewed
• [Journal Article] New Molecular Mechanisms for Cardiovascular Disease:Cardiac Hypertrophy and Cell-Volume Regulation (2011)
  • Author(s): Yamamoto S, Kita S, Iyoda T, Yamada T, Iwamoto T.
  • Journal Title: Journal of Pharmacological Sciences Volume: 116 Issue: 4 Pages: 343-349
  • DOI: 10.1254/jphs.10R31FM
  • NAID: 10029895523
  • ISSN: 1347-8613, 1347-8648
  • Peer Reviewed
• [Journal Article] Role of Na+/Ca2+ exchanger type 1 (NCX1) in theangiogenesis induced by Lipo-PGE1 in murine hindlimb ischemia model. (2011)
  • Author(s): Matsui Y, Kita S, Iyoda T, Katsuragi T, Komuro I, Iwamoto T, Ohjimi H.
  • Journal Title: Med Bull Fukuoka Univ Volume: 38(2) Pages: 77-84
  • Peer Reviewed
• [Journal Article] Cilostazol suppresses angiotensin II-induced vasoconstriction via protein kinase A-mediated phosphorylation of the transient receptor potential canonical 6 channel (2011)
  • Author(s): Nishioka, K.
  • Journal Title: Arterioscler Thromb Vasc Biol Volume: 31 Pages: 2278-2286
  • Peer Reviewed
• [Journal Article] TRIC-A channels in vascular smooth muscle contribute to blood pressure maintenance (2011)
  • Author(s): Yamazaki, D.
  • Journal Title: Cell Metab Volume: 14 Pages: 231-241
  • Peer Reviewed
• [Journal Article] OSU-03012, a novel celecoxib derivative, induces cell swelling and shortens action potential duration in mouse ventricular cells (2010)
  • Author(s): Yamamoto S, Iyoda T, Kita S, Yamada T, Iwamoto T.
  • Journal Title: Biomedical Research Volume: 31 Issue: 6 Pages: 413-417
  • DOI: 10.2220/biomedres.31.413
  • NAID: 130004470768
  • ISSN: 0388-6107, 1880-313X
  • Peer Reviewed
• [Journal Article] Cellular Ca2+ Dynamics in Urinary Bladder Smooth Muscle From Transgenic Mice Overexpressing Na+-Ca2+ Exchanger (2010)
  • Author(s): Murata H, Hotta S, Sawada E, YamamuraH, Ohya S, Kita S, Iwamoto T, ImaizumiY.
  • Journal Title: Journal of Pharmacological Sciences Volume: 112 Issue: 3 Pages: 373-377
  • DOI: 10.1254/jphs.09319SC
  • NAID: 130000253493
  • ISSN: 1347-8613, 1347-8648
  • Peer Reviewed
• [Journal Article] Cellular Ca^<2+> dynamics in urinary bladder smooth muscle from transgenic mice overexpressing Na^+-Ca^<2+> exchanger. (2010)
  • Author(s): Murata, H.
  • Journal Title: T Pharmacol Sci Volume: 112 Pages: 373-377
  • Peer Reviewed
• [Journal Article] OSU-03012, a novel celecoxib derivative, induces cell swelling and shortens action potential duration in mouse ventricular cells. (2010)
  • Author(s): Yamamoto, S.
  • Journal Title: Biomed Res Volume: 31 Pages: 413-417
  • NAID: 130004470768
  • Peer Reviewed
• [Journal Article] Mechanisms, sms for linking high salt intake to vascular tone : role of Na^+ pumpand Na^+/Ca^<2+> exchanger coupling. (2010)
  • Author(s): Kita, S.
  • Journal Title: Yakugaku Zasshi Volume: 130 Pages: 1399-1405
  • Peer Reviewed
• [Presentation] Ca2+ mobilization mechanisms through TRPCs and NCX1 in regulation of vascular tonus. (2013)
  • Author(s): Kita, S.
  • Organizer: 第86回日本薬理学会年会
  • Place of Presentation: 福岡
  • Year and Date: 2013-03-22
• [Presentation] Preferential involvement ofNCX1 in the brain damage caused bytransient focal cerebral ischemia in mice. (2013)
  • Author(s): Kita, S
  • Organizer: Biophysical Society 57th Annual Meeting
  • Place of Presentation: Philadelphia(米国)
  • Year and Date: 2013-02-04
• [Presentation] Preferential involvement of NCX1 in the brain damage caused by transient focal cerebral ischemia in mice (2013)
  • Author(s): Kita, S.
  • Organizer: Biophysical Society 57th Annual Meeting
  • Place of Presentation: Philadelphia, USA
• [Presentation] Ca2+ mobilization mechanisms through TRPCs and NCX1 in regulation of vascular tonus (2013)
  • Author(s): Kita, S.
  • Organizer: 第86回日本薬理学会年会
  • Place of Presentation: 福岡
• [Presentation] マウス腸間膜細動脈の筋原性収縮およびα1アゴニスト収縮におけるTRPC3/6の役割 (2012)
  • Author(s): 喜多紗斗美
  • Organizer: 第65回日本薬理学会西南部会
  • Place of Presentation: 熊本
  • Year and Date: 2012-11-23
• [Presentation] Vascular NCX1 contributes to hypertension inpseudohypoaldosteronism type II and cushing's syndrome models (2012)
  • Author(s): 後藤雄輔
  • Organizer: 第85回日本薬理学会年会
  • Place of Presentation: 京都
  • Year and Date: 2012-03-15
• [Presentation] Vascular NCX1 contributes to hypertension in pseudohypoaldosteronism type II and cushing's syndrome models (2012)
  • Author(s): 後藤雄輔
  • Organizer: 第85回日本薬理学会年会
  • Place of Presentation: 京都国際会館(京都)
  • Year and Date: 2012-03-15
• [Presentation] Vascular Na+/Ca2+ exchanger type-1 contributes to hypertension in pseudohypoaldosteronism type II and Cushing's syndrome models. (2012)
  • Author(s): Kita, S.
  • Organizer: Biophysical Society 56th Annual Meeting
  • Place of Presentation: San Diego(米国)
  • Year and Date: 2012-02-29
• [Presentation] Vascular Na^+/Ca^<2+> exchanger type-1 contributes to hypertension in pseudohypoaldosteronism type II and Cushing's syndrome models (2012)
  • Author(s): Kita, S.
  • Organizer: Biophysical Society 56th Annual Meeting
  • Place of Presentation: San Diego(米国)
  • Year and Date: 2012-02-29
• [Presentation] Involvement of TRPC3 and TRPC6 channels in myogenic tone regulation of murine mesenteric arteries. (2012)
  • Author(s): Kita, S.
  • Organizer: 第35回日本分子生物学会大会
  • Place of Presentation: 福岡
• [Presentation] Involvement of TRPC3 and TRPC6 channels in myogenic tone regulation of murine mesenteric arteries (2012)
  • Author(s): Kita, S.
  • Organizer: 第35回日本分子生物学会大会
  • Place of Presentation: 福岡
• [Presentation] The role of NCX1 in pseudohypoaldosteronism type II and ACTH-induced hypertension. (2011)
  • Author(s): Kita, S.
  • Organizer: 第34回日本分子生物学会大会
  • Place of Presentation: 神奈川
  • Year and Date: 2011-12-14
• [Presentation] The role of NCX1 in pseudohypoaldosteronism type II and ACTH-induced hypertension (2011)
  • Author(s): Kita, S.
  • Organizer: 第34回日本分子生物学会大会
  • Place of Presentation: パシフィコ横浜(神奈川)
  • Year and Date: 2011-12-14
• [Presentation] ACTH誘発性およびPHAII型高血圧におけるNCX1の役割 (2011)
  • Author(s): 喜多紗斗美
  • Organizer: 第64回日本薬理学会西南部会
  • Place of Presentation: 福岡
  • Year and Date: 2011-11-20
• [Presentation] ACTH誘発性およびPHAII型高血圧におけるNCX1の役割 (2011)
  • Author(s): 喜多紗斗美
  • Organizer: 第64回日本薬理学会西南部会
  • Place of Presentation: KKRホテル博多(福岡)
  • Year and Date: 2011-11-20
• [Presentation] Na+/Ca2+交換輸送体をめぐるトランスポートソーム (2011)
  • Author(s): 喜多紗斗美
  • Organizer: 第84回日本薬理学会年会
  • Place of Presentation: 神奈川
  • Year and Date: 2011-03-23
• [Presentation] Na^+/Ca^<2+>交換輸送体をめぐるトランスポートソーム (2011)
  • Author(s): 喜多紗斗美
  • Organizer: 第84回日本薬理学会年会
  • Place of Presentation: パシフィコ横浜(神奈川)
  • Year and Date: 2011-03-23
• [Presentation] Caveolin-3 regulates the volume-regulated anion channel in mouse ventricular cells. (2011)
  • Author(s): Yamamoto, S.
  • Organizer: 55th Annual Meeting of the Biophysical Society
  • Place of Presentation: Baltimore(米国)
  • Year and Date: 2011-03-07
• [Presentation] Caveolin-3 regulates the volume-regulated anion channel in mouse ventricular cells (2011)
  • Author(s): Yamamoto, S.
  • Organizer: 55^<th> Annual Meeting of the Biophysical Society
  • Place of Presentation: Baltimore(USA)
  • Year and Date: 2011-03-07
• [Presentation] 心不全発症におけるPIP5キナーゼの役割について (2010)
  • Author(s): 喜多紗斗美
  • Organizer: 平成22年度生理研研究会
  • Place of Presentation: 愛知
  • Year and Date: 2010-11-05
• [Presentation] 心不全発症におけるPIP5キォーゼの役割について (2010)
  • Author(s): 喜多紗斗美
  • Organizer: 平成22年度生理研研究会
  • Place of Presentation: 生理学研究所(愛知)
  • Year and Date: 2010-11-05
• [Presentation] PIP5キナーゼのイオン輸送体制御を介する心肥大のメカニズム (2010)
  • Author(s): 喜多紗斗美
  • Organizer: 第4回トランスポーター研究会九州部会
  • Place of Presentation: 長崎
  • Year and Date: 2010-09-11
• [Presentation] PIP5キナーゼのイオン輸送体制御を介する心肥大のメカニズム (2010)
  • Author(s): 喜多紗斗美
  • Organizer: 第4回トランスポーター研究会九州部会
  • Place of Presentation: ハウステンボスユトレヒト会議室(長崎)
  • Year and Date: 2010-09-11
• [Presentation] Role of functional coupling between TRPC3 and NCX1 in sympathetic vasoconstriction. (2010)
  • Author(s): 喜多紗斗美
  • Organizer: 第87回日本生理学会大会
  • Place of Presentation: 岩手
  • Year and Date: 2010-05-21
• [Presentation] Role of functional coupling between TRPC3 and NCX1 in sympathetic vaso constriction (2010)
  • Author(s): 喜多紗斗美
  • Organizer: 第87回日本生理学会大会
  • Place of Presentation: 盛岡市民文化ホール(岩手)
  • Year and Date: 2010-05-21
• [Presentation] Na+/Ca2+ exchanger and oxidative stress inaldosterone-induced cardiac remodeling (2010)
  • Author(s): Iyoda, T.
  • Organizer: XXth ISHR World Congress2010 KYOTO
  • Place of Presentation: 京都
  • Year and Date: 2010-05-14
• [Presentation] Cardiac-specific overexpression of NCX1.1 mutant induces dilated cardiomyopathy in mice. (2010)
  • Author(s): Kita, S
  • Organizer: XXth ISHR World Congress 2010 KYOTO
  • Place of Presentation: 京都
  • Year and Date: 2010-05-14
• [Presentation] Cardiac-specific overexpression of NCX1.1 mutant induces dilated cardimyopathy in mice (2010)
  • Author(s): Kita, S.
  • Organizer: XXth ISHR World Congress 2010 KYOTO
  • Place of Presentation: 京都国際会館(京都)
  • Year and Date: 2010-05-14
• [Presentation] Na^+/Ca^<2+> exchanger and oxidative stress in aldosterone-induced cardiac remodeling (2010)
  • Author(s): Iyoda, T.
  • Organizer: XXth ISHR World Congress 2010 KYOTO
  • Place of Presentation: 京都国際会館(京都)
  • Year and Date: 2010-05-13
• [Presentation] Involvement of TRPC3 and TRPC6 channels in myogenic tone regulation of murine mesenteric arteries
  • Author(s): Kita, S.
  • Organizer: 第65回日本薬理学会西南部会
  • Place of Presentation: 熊本
• [Book] トランスポートソームの世界 (2011)
  • Author(s): 金井好克/竹島浩/森泰生/久保義弘
  • Total Pages: 471
  • Publisher: 京都広川書店
• [Remarks]
  • URL: http://www.med.fukuoka-u.ac.jp/
• [Remarks] 福岡大学医学部薬理学教室
  • URL: http://www.med.fukuoka-u.ac.jp/pharmaco/index.html
• [Remarks]
  • URL: http://www.med.fukuoka-u.ac.jp/
• [Remarks]
  • URL: http://www.med.fukuoka-u.ac.jp/