Functional analysis of CIBZ, a methyl-CpG binding protein which is essential for the ES cell differentiation

• Project/Area Number: 22590272
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General medical chemistry
• Research Institution: Nara Institute of Science and Technology
• Principal Investigator: MATSUDA Eishou 奈良先端科学技術大学院大学, バイオサイエンス研究科, 助教 (00335481)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: ES 細胞 / 細胞増殖 / 細胞分化 / 細胞周期 / DNA メチル化 / 筋芽細胞分化 / 胚性幹（ES）細胞 / DNAメチル化 / メチル化DNA結合タンパク質 / 転写後修飾 / Nanog / ES細胞 / 細胞死 / 転写制御

Research Abstract

G1/S transition is important for embryonic stem cell (ESC) proliferation; however, the transcriptional factor that regulates this remains largely unknown. We present data showing that CIBZ, a transcription factor, regulates ESC proliferation and G1/S transition. Our results indicate that CIBZ-associated ESC proliferation and G1/S transition is dependent on Nanog expression.

Research Products

• [Journal Article] CtBP-interacting BTB zinc finger protein (CIBZ) promotes proliferation and G1/S transition in embryonic stem cells via Nanog (2012)
  • Author(s): Tomonori Nishii, Yu Oikawa, Yasumasa Ishida, Masashi Kawaichi and Eishou Matsuda
  • Journal Title: The Journal of Biological Chemistry Volume: 287巻 Issue: 15 Pages: 12417-12424
  • DOI: 10.1074/jbc.m111.333856
  • NAID: 120005818445
  • Peer Reviewed
• [Journal Article] The methyl-CpG-binding protein CIBZ suppresses myogenic differentiation by directly inhibiting myogenin expression (2011)
  • Author(s): Yu Oikawa, Reiko Omori, Tomonori Nishii, Yasumasa Ishida, Masashi Kawaichi and Eishou Matsuda
  • Journal Title: Cell Research Volume: 21巻 Issue: 11 Pages: 1578-1590
  • DOI: 10.1038/cr.2011.90
  • NAID: 120005818451
  • Peer Reviewed
• [Presentation] DNA methylation and myoblast differentiation: role of a new methyl-binding protein (2011)
  • Author(s): 松田永照
  • Organizer: BIT's 1st Annual World Congress of Molecular & Cell Biology
  • Place of Presentation: 中国北京市北京国際会議中心
  • Year and Date: 2011-08-07
• [Presentation] DNA methylation and myoblast differentiation : role of a new methyl-binding protein (2011)
  • Author(s): Eishou Matsuda
  • Organizer: BIT's 1st Annual World Congress of Molecular & Cell Biology
  • Place of Presentation: 中国北京市北京国際会議中心
  • Year and Date: 2011-08-07