Elucidation of the mechanism underlying differentiation and function of Leydig cells through identification of enhanceosome.

• Project/Area Number: 22590273
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General medical chemistry
• Research Institution: Kyushu University
• Principal Investigator: BABA Takashi 九州大学, 大学院・医学研究院, 助教 (40435524)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 核内受容体 / エネルギーホメオスタシス / レギュロン / エンハンサー / 転写制御 / ステロイドホルモン / ライディッヒ細胞 / Ad4BP/SF-1 / クロマチン免疫沈降 / 転写複合体 / トランスジェニックマウス

Research Abstract

I investigated the mechanism of the transcriptional regulation of Ad4BP/SF-1 gene in Leydig cells. As a result, it was revealed that the expression of Ad4BP/SF-1 gene is regulated by Ad4BP/SF-1 itself. Further, I elucidated through a genome-wide analysis of target genes of Ad4BP/SF-1 that genes involved in the glycolytic pathway were targets of Ad4BP/SF-1.

Research Products

• [Journal Article] Steroid hormone and the development of reproductive organs (2013)
  • Author(s): K. Morohashi, T. Baba, M. Tanaka
  • Journal Title: Sex. Dev Volume: 7 Pages: 61-79
• [Journal Article] Contribution of Leydig and Sertoli cells to testosterone production in mouse fetal testes (2013)
  • Author(s): Shima Y, Miyabayashi K, Haraguchi S, Arakawa T, Otake H, Baba T, Matsuzaki S, Shishido Y, Akiyama H, Tachibana T, Tsutsui K, Morohashi K
  • Journal Title: Mol. Endocrinol Volume: 27 Pages: 63-73
  • Peer Reviewed
• [Journal Article] Steroid hormone and the development of reproductive organs. (Invited Review) (2013)
  • Author(s): Morohashi, K., Baba,T. and Tanaka, M.
  • Journal Title: Sexual Development Volume: 7 Issue: 1-3 Pages: 61-79
  • DOI: 10.1159/000342272
  • Peer Reviewed
• [Journal Article] Contribution of Leydig and Sertoli cells to testosterone production in mouse fetal testes (2013)
  • Author(s): Yuichi Shima
  • Journal Title: Molecular Endocrinology Volume: 27 Issue: 1 Pages: 63-73
  • DOI: 10.1210/me.2012-1256
  • Peer Reviewed
• [Journal Article] Identification of an enhancer in the Ad4BP/SF-1 gene specific for fetal Leydig cells (2012)
  • Author(s): Shima Y, Miyabayashi K, Baba T, Otake H, Oka S, Zubair M, Morohashi K
  • Journal Title: Endocrinology Volume: 153 Pages: 417-425
  • Peer Reviewed
• [Journal Article] Cbx2, a polycomb group gene, is required for Sry gene expression in mice (2012)
  • Author(s): Katoh-Fukui Y, Miyabayashi K, Komatsu T, Owaki A, Baba T, Shima Y, Kidokoro T, Kanai Y, Schedl A, Wilhelm D, Koopman P, Okuno Y, Morohashi K
  • Journal Title: Endocrinology Volume: 153 Pages: 913-924
  • Peer Reviewed
• [Journal Article] Global DNA methylation in the mouse liver is affected by methyl deficiency and arsenic in a sex-dependent manner (2011)
  • Author(s): Nohara K, Baba T, Murai H, Kobayashi Y, Suzuki T, Tateichi Y, Matsumoto M, Nishimura N, Sano T
  • Journal Title: Arch. Toxicol Volume: 85 Pages: 653-661
  • Peer Reviewed
• [Journal Article] Abnormal epithelial cell polarity and migration of Emx2 KO embryonic gonads induced by ectopic EGFRexpression (2010)
  • Author(s): Masatomo Kusaka, Yuko Katoh-Fukui, Hidesato Ogawa, Kanako Miyabayashi, Takashi Baba,Yuichi Shima, Noriyuki Sugiyama, Yukihiko Sugimoto, Yasushi Okuno, Ryuji Kodama, Akiko Iizuka-Kogo, Takeo Senda, Toshikuni Sasaoka, Kunio Kitamura, Shinichi Aizawa, Ken-ic
  • Journal Title: Endocrinology Volume: 151 Pages: 5893-5904
  • Peer Reviewed
• [Journal Article] Abnormal epithelial cell polarity and migration of Emx2 KO embryonic gonads induced by ectopic EGFR expression. (2010)
  • Author(s): Masatomo Kusaka, Yuko Katoh-Fukui, Hidesato Ogawa, Kanako Miyabayashi, Takashi Baba, et al.
  • Journal Title: Endocrinology Volume: 151 Pages: 5893-5904
  • Peer Reviewed
• [Journal Article] Global DNA methylation in the mouse liver is affected by methyl deficiency and arsenic in a sex-dependent manner. (2010)
  • Author(s): Nohara K, Baba T, Murai H, Kobayashi Y, Suzuki T, et al.
  • Journal Title: Arch Toxicol. Volume: (印刷中)
  • Peer Reviewed
• [Presentation] Deep sequencing から見えてきたAd4BP/SF-1 の多才性: グルコース代謝の組織特異的制御 (2013)
  • Author(s): 馬場崇
  • Organizer: 第5回核内受容体・ホルモン研究会
  • Place of Presentation: 霞が関コモンゲート西館
  • Year and Date: 2013-03-15
• [Presentation] 組織特異的転写因子Ad4BP/SF-1による解糖系遺伝子群の転写を介したグルコース代謝制御 (2013)
  • Author(s): 馬場崇
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場
• [Presentation] Deep sequencing から見えてきた Ad4BP/SF-1 の多才性: グルコース代謝の組織特異的制御 (2013)
  • Author(s): 馬場崇
  • Organizer: 第5回 核内受容体・ホルモン研究会
  • Place of Presentation: 霞ヶ関コモンゲート西館
  • Invited
• [Presentation] 6th International Symposium on the Biology of Vertebrate Sex Determination (2012)
  • Author(s): Identification of Ad4BP/SF-1 target genes by mRNA-seq and ChIP-seq in Y-1 adrenocortical cellsTakashi Baba
  • Organizer: King Kamehameha's Kona Beach
  • Place of Presentation: Hotel, KONA, HAWAII
• [Presentation] 核内受容体Ad4BP/SF-1の副腎皮質における新たな機能 (2012)
  • Author(s): 馬場崇
  • Organizer: 第85回日本内分泌学会学術総会
  • Place of Presentation: 名古屋国際会議場
• [Presentation] Identification of Ad4BP/SF-1 target genes by mRNA-seq and ChIP-seq in Y-1 adrenocortical cells (2012)
  • Author(s): 馬場崇
  • Organizer: 6th International Symposium on the Biology of Vertebrate Sex Determination
  • Place of Presentation: King Kamehameha's Kona Beach Hotel, KONA, HAWAII
• [Presentation] Identification of Ad4BP/SF-1 target genes by ChIP-seq in Y-1 adrenocortical cells (2011)
  • Author(s): 馬場崇
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2011-12-16
• [Presentation] Identification of Ad4BP/SF-1 target genes by ChIP-Seq in Y-1 adrenocortical cells (2011)
  • Author(s): 馬場崇、大竹博之、宮林香奈子、嶋雄一、佐藤哲也、木村宏、大川恭行、須山幹太、諸橋憲一郎
  • Organizer: 第34回日本分子生物学会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2011-12-16
• [Presentation] RNA-seq およびChIP-seq 法によるAd4BP/SF-1標的遺伝子の同定 (2011)
  • Author(s): 馬場崇
  • Organizer: 第4回性差生物医学研究会
  • Place of Presentation: 山口大学医学部霜仁会館
  • Year and Date: 2011-11-21
• [Presentation] RNA-SeqおよびChIP-Seq法によるAd4BP/SF-1標的遺伝子の同定 (2011)
  • Author(s): 馬場崇、大竹博之、宮林香奈子、嶋雄一、佐藤哲也、木村宏、大川恭行、須山幹太、諸橋憲一郎
  • Organizer: 第4回性差生物医学研究会
  • Place of Presentation: 山口大学医学部霜仁会館
  • Year and Date: 2011-11-21
• [Remarks]
  • URL: http://www.med.kyushu-u.ac.jp/seisaseibutu/
• [Remarks] 九州大学大学院 医学研究院 分子生命科学系部門 性差生物学研究室ホームページ
  • URL: http://www.med.kyushu-u.ac.jp/seisaseibutu/
• [Remarks]
  • URL: http://www.med.kyushu-u.ac.jp/seisaseibutu/project.html