Recruitment of Polycomb-group complex for stable repressions
| Project/Area Number |
22590278
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General medical chemistry
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| Research Institution | The Institute of Physical and Chemical Research |
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Principal Investigator |
ISONO Kyoichi 独立行政法人理化学研究所, 免疫器官形成研究グループ, 上級研究員 (90323435)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | ポリコーム / 発現制御 / 遺伝子制御 / エピジェネティクス / 核内ドメイン / ポリコーム群 / クロマチン / 細胞イメージング / 核ドメイン |
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| Research Abstract |
To better understand recruitment mechanisms of PRC1, we focused on subnuclear domains consisting of PRC1. To data H3K27me3 is known to be one of factors for PRC1 recruitment. Conditional deletion experiments of Ezh2 however did not support that H3K27me3 was a critical recruiter of PRC1. On the other hand, FRAP analysis showed that about 20% of PRC1 component was immobile on chromatin, implying that such an immobile fraction becomes a memory tag of PRC1 beyond cell division. We thus have been addressing this hypothesis
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Report
(4 results)
Research Products
(14 results)
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[Journal Article] Histone H2A Mono-Ubiquitination Is a Crucial Step to Mediate PRC1-Dependent Repression of Developmental Genes to Maintain ES Cell Identity.2012
Author(s)
Endoh M, Endo TA, Endoh T, Isono K, Sharif J, Ohara O, Toyoda T, Ito T, Eskeland R, Bickmore WA, Vidal M, Bernstein BE, Koseki H.
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Journal Title
PLoS Genet.
Volume: 8
Issue: 7
Pages: e1002774-e1002774
DOI
NAID
Related Report
Peer Reviewed
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