| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Research Abstract |
To better understand recruitment mechanisms of PRC1, we focused on subnuclear domains consisting of PRC1. To data H3K27me3 is known to be one of factors for PRC1 recruitment. Conditional deletion experiments of Ezh2 however did not support that H3K27me3 was a critical recruiter of PRC1. On the other hand, FRAP analysis showed that about 20% of PRC1 component was immobile on chromatin, implying that such an immobile fraction becomes a memory tag of PRC1 beyond cell division. We thus have been addressing this hypothesis
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