Regulation of stress activated protein kinase pathway by protein phosphatase 2C

• Project/Area Number: 22590280
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Pathological medical chemistry
• Research Institution: Tohoku University
• Principal Investigator: TAMURA Shinri 東北大学, 加齢医学研究所, 教授 (20124604)
• Co-Investigator(Kenkyū-buntansha): KOBAYASHI Takayasu 東北大学, 加齢医学研究所, 准教授 (10221970)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: プロテインホスファターゼ / PP2C / 小胞体 / ストレス応答 / 小胞体ストレス / PP2Cε / INK / IRE1α

Research Abstract

In this study we identified acyl CoA binding domain containing 3 (ACBD3) as a binding partner of PPM1L and suggested that ACBD3 recruits PPM1L to the ER-Golgi membrane contact site. We also demonstrated that PPM1L interacts with GOLD(Golgi dynamics) domain that exists in several proteins involved in Golgi function.

Research Products

• [Journal Article] N-Myristoylation is essential for protein phosphatases PPM1A and PPM1B to dephosphorylate their physiological substrates in cells (2013)
  • Author(s): Chida, T.
  • Journal Title: Biochem J Volume: 449 Issue: 3 Pages: 741-749
  • DOI: 10.1042/bj20121201
  • Peer Reviewed
• [Journal Article] Acyl-CoA binding domain containing 3 (ACBD3) recruits the protein phosphatase PPM1L to ER-Golgi membrane contact sites (2012)
  • Author(s): Shinoda, Y.
  • Journal Title: FEBS Letter Volume: 586 Issue: 19 Pages: 3024-3029
  • DOI: 10.1016/j.febslet.2012.06.050
  • Peer Reviewed
• [Journal Article] Shigeru Tsuiki : a pioneer in the research fields of complex carbohydrates and protein phosphatases (2011)
  • Author(s): T.Miyagi, et. al.
  • Journal Title: J.Biochem. Volume: in press
  • Peer Reviewed
• [Journal Article] TGF-β-activated kinase 1 mediates mechanical stress-induced IL-6 expression in osteoblasts (2011)
  • Author(s): N.Fukuno, et. al.
  • Journal Title: Biochem.Biophys.Res.Commun. Volume: in press
  • Peer Reviewed
• [Presentation] Golgi complex-associated protein of 60 kDa (ACBD3) recruits the protein phosphatase PPM1L to ER-Golgi membrane contact sites (2013)
  • Author(s): Shinoda,Y
  • Organizer: 10th International Conference on Protein Phosphatase
  • Place of Presentation: 東京
• [Presentation] Targeted disruption of the mouse protein phosphatase 2Cε gene leads to structural abnormalities in the brain (2013)
  • Author(s): Fujita, K
  • Organizer: 10th International Conference on Protein Phosphatase
  • Place of Presentation: 東京
• [Presentation] Golgi complex-associated protein of 60 kDa (GCP60) recruits the protein phosphatase PPM1L to ER-Golgi membrane contact sites (2013)
  • Author(s): Shinoda, Y.
  • Organizer: 10th International Conference on Protein Phosphatase
  • Place of Presentation: Tokyo
• [Presentation] Targeted disruption of the mouse protein phosphatase 2Cε gene leads to structural abnormalities in the brain (2013)
  • Author(s): Fujita, K.
  • Organizer: 10th International Conference on Protein Phosphatase
  • Place of Presentation: Tokyo
• [Presentation] Golgi complex-associated protein of 60 kDa (GCP60) recruits the protein phosphatase PPM1L to ER-Golgi membrane contact sites (2013)
  • Author(s): 篠田康晴
  • Organizer: 10th International Conference on Protein Phosphatase
  • Place of Presentation: 東京
• [Presentation] プロテインホスファターゼPP2Cεによる小胞体ストレス応答の制御 (2012)
  • Author(s): 永浦裕子
  • Organizer: 日本生化学会東北支部第78回例会
  • Place of Presentation: 山形
  • Year and Date: 2012-05-26
• [Presentation] Golgi complex-associated protein of 60 kDa (ACBD3) recruits the protein phosphatase PPM1L toER-Golgi membrane contact sites (2012)
  • Author(s): 篠田康晴
  • Organizer: 第35回日本分子生物学会総会
  • Place of Presentation: 福岡
• [Presentation] ノックアウトマウスを用いたPP2Cεの新規機能解明 (2012)
  • Author(s): 藤田宏介
  • Organizer: 第35回日本分子生物学会総会
  • Place of Presentation: 福岡
• [Presentation] プロテインホスファターゼ2Cによる細胞機能の制御 (2012)
  • Author(s): 田村眞理
  • Organizer: 第5回日本プロテインホスファターゼ研究会学術集会
  • Place of Presentation: 大阪
• [Presentation] N-ミリストイル化はPP2CαおよびPP2Cβの機能発揮に必須である (2012)
  • Author(s): 小林孝安
  • Organizer: 第5回日本プロテインホスファターゼ研究会学術集会
  • Place of Presentation: 大阪
• [Presentation] ノックアウトマウスを用いたPP2Cεの新規機能解明 (2012)
  • Author(s): 藤田宏介
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
• [Presentation] Golgi complex-associated protein of 60 kDa (GCP60) recruits the protein phosphatase PPM1L to ER-Golgi membrane contact sites (2012)
  • Author(s): 篠田康晴
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
• [Presentation] Golgi complex-associated protein of 60 kDa (GCP60) recruits the protein phosphatase PPM1L to ER-Golgi membrane contact sites (2012)
  • Author(s): 篠田康晴
  • Organizer: 第35回日本分子生物学会総会
  • Place of Presentation: 福岡
• [Presentation] Regulation of Cellular Functions by PP2C Family Members (2011)
  • Author(s): 田村眞理
  • Organizer: 発がんスパイラル第1回国際シンポジウム&第9回プロテインボスファターゼ国際カンファランス
  • Place of Presentation: 東京
  • Year and Date: 2011-02-01
• [Presentation] AMP Activated Protein Kinase (AMPK) is Regulated by Multiple Protein Phosphatase (2011)
  • Author(s): 小林孝安
  • Organizer: 発がんスパイラル第1回国際シンポジウム&第9回プロテインボスファターゼ国際カンファランス
  • Place of Presentation: 東京
  • Year and Date: 2011-02-01
• [Presentation] PP2Cδ/ILKAPはcaspase-3の新たな基質である (2011)
  • Author(s): 神藤佑亮
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜
• [Presentation] PP2Cδ/ILKAP stabilizes p53 through regulation of the Akt-GSK3β pathway (2011)
  • Author(s): 千田透子
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜
• [Presentation] Mechanobiological regulation of bone remodeling cycle by the expression of cytokine-related genes via JNK and p38 inosteoblast (2011)
  • Author(s): 松井裕之
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜
• [Presentation] N-ミリストイル化はPP2Cαおよびβの機能発揮に必須である (2011)
  • Author(s): 小林孝安
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 京都
• [Presentation] AMPK activated Protein Kinase (AMPK)の脱リン酸化を担うプロテインホスファターゼの解析 (2010)
  • Author(s): 小林孝安
  • Organizer: 第33回日本分子生物学会・第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-10
• [Presentation] 骨芽細胞のJNKおよびp38はメカノバイオロジーにおいて破骨細胞とのサイトカインクロストークに寄与する (2010)
  • Author(s): 松井裕之
  • Organizer: 第33回日本分子生物学会・第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-07
• [Presentation] PP2CζはJNKにより負の制御を受ける (2010)
  • Author(s): 小林孝安
  • Organizer: 日本生化学会東北支部第76回例会・シンポジウム
  • Place of Presentation: 福島
  • Year and Date: 2010-05-08