Investigation of the regulatory mechanisms of cellular energy metabolism by snail
| Project/Area Number |
22590287
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pathological medical chemistry
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| Research Institution | Kagoshima University |
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Principal Investigator |
HARAGUCHI Misako 鹿児島大学, 大学院・医歯学総合研究科, 准教授 (10244229)
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| Co-Investigator(Kenkyū-buntansha) |
OZAWA Masayuki 鹿児島大学, 大学院・医歯学総合研究科, 教授 (90136854)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | Snail / ピルビン酸デヒドロゲナーゼキナーゼ(PDK1) / VLCAD / ATPクエン酸リアーゼ / ATP / グルコース / snail / ピルビン酸デヒドロゲナーゼ / MDCK / スネイル / ピルビン酸デヒドロゲナーゼキナーセ / ワールブルグ効果 / 酸化的リン酸化 / 解糖系 |
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| Research Abstract |
Snail expression decreases oxidative phosphorylation and increases glucose dependency in MDCK cells. MDCK/Snail cells exhibited reduced activity of pyruvate dehydrogenase (PDH), which controls pyruvate entry into the TCA cycle, and increased promoter activity and expression of pyruvate dehydrogenase kinase 1 (PDK1), which inhibits PDH. In addition, the activities of TCA cycle enzymes were decreased in MDCK/snail cells. Consequently, MDCK/snail cells exhibited lower O2 consumption and ATP levels than MDCK/neo cells. MDCK/snail cells also showed reduced expression of enzymes essential for glutaminolysis and fatty acid synthesis.
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Report
(4 results)
Research Products
(13 results)
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[Journal Article] Akiyama S, Thymidine phosphorylase enhances reactive oxygen species generation and interleukin-8 expression in human cancer cells2012
Author(s)
Tabata S, Ikeda R, Yamamoto M, Furukawa T, Kuramoto T, Takeda Y, Yamada K, Haraguchi M, Nishioka Y, Sone S
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Journal Title
Oncol Rep
Volume: 28
Pages: 895-902
DOI
Related Report
Peer Reviewed
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