| Project/Area Number |
22590287
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Pathological medical chemistry
|
|---|
| Research Institution | Kagoshima University |
|---|
Principal Investigator |
HARAGUCHI Misako 鹿児島大学, 大学院・医歯学総合研究科, 准教授 (10244229)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
OZAWA Masayuki 鹿児島大学, 大学院・医歯学総合研究科, 教授 (90136854)
|
|---|
| Project Period (FY) |
2010 – 2012
|
| Project Status |
Completed (Fiscal Year 2012)
|
| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
|
|---|
| Keywords | Snail / ピルビン酸デヒドロゲナーゼキナーゼ(PDK1) / VLCAD / ATPクエン酸リアーゼ / ATP / グルコース / snail / ピルビン酸デヒドロゲナーゼ / MDCK / スネイル / ピルビン酸デヒドロゲナーゼキナーセ / ワールブルグ効果 / 酸化的リン酸化 / 解糖系 |
|---|
| Research Abstract |
Snail expression decreases oxidative phosphorylation and increases glucose dependency in MDCK cells. MDCK/Snail cells exhibited reduced activity of pyruvate dehydrogenase (PDH), which controls pyruvate entry into the TCA cycle, and increased promoter activity and expression of pyruvate dehydrogenase kinase 1 (PDK1), which inhibits PDH. In addition, the activities of TCA cycle enzymes were decreased in MDCK/snail cells. Consequently, MDCK/snail cells exhibited lower O2 consumption and ATP levels than MDCK/neo cells. MDCK/snail cells also showed reduced expression of enzymes essential for glutaminolysis and fatty acid synthesis.
|
