Study for metabolic regulatory mechanism by a mitochondrial protein and a sex hormone

• Project/Area Number: 22590290
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Pathological medical chemistry
• Research Institution: Jichi Medical University
• Principal Investigator: ENDO Hitoshi 自治医科大学, 医学部, 教授 (50221817)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: ミトコンドリア / プロヒビチン / 性ホルモン / 核-ミトコンドリア連関 / 脂肪分化 / プロテオーム / 核―ミトコンドリア連関 / 質量分析

Research Abstract

Prohibitin 2 (PHB2) has been reported as a multifunctional protein that mainly localizes in mitochondria and translocates into nucleus in the presence of estrogen receptor and estrogen. Decrease of estrogen level is a major cause of obesity in early post-

Research Products

• [Journal Article] Translational repression of the McKusick-Kaufman syndrome transcript by unique upstream open reading frames encoding mitochondrial proteins with alternative polyadenylation sites (2013)
  • Author(s): Akimoto, C. Sakashita, E., Kasashima, K., Kuroiwa, K., Tominaga, K., Hamamoto, T., and Endo, H.
  • Journal Title: Biochem Biophys Acta Volume: 1830 Issue: 3 Pages: 2728-2738
  • DOI: 10.1016/j.bbagen.2012.12.010
  • Peer Reviewed
• [Journal Article] Epitope determination of anti Thy-1 monoclonal antibodies that regulate neurite outgrowth (2012)
  • Author(s): Kuroiwa, K., Torkai,Y., Osawa, M., Nakashima, T., Nakashima, M., Endo, H., and Arai, T
  • Journal Title: Hybridoma Volume: 31 Issue: 4 Pages: 225-232
  • DOI: 10.1089/hyb.2012.0002
  • Peer Reviewed
• [Journal Article] Maintenance of mitochondrial genome distribution by mitochondrial AAA+ protein.ClpX. Exp (2012)
  • Author(s): Kasashima, K., Sumitani, M., and Endo, H
  • Journal Title: Cell Res Volume: 318 Issue: 18 Pages: 2335-2343
  • DOI: 10.1016/j.yexcr.2012.07.012
  • Peer Reviewed
• [Journal Article] ミトコンドリア膜の機能的新タンパク質類―プロヒビチン (2012)
  • Author(s): 遠藤仁司
  • Journal Title: 生体の科学 Volume: 63 Pages: 432-433
• [Journal Article] Human mitochondrial transcription factor A is required for the segregation of mitochondrial DNA in cultured cells (2011)
  • Author(s): Kasashima, K., Sumitani, M., and Endo, H
  • Journal Title: Exp. Cell Res. Volume: 317(2) Issue: 2 Pages: 210-220
  • DOI: 10.1016/j.yexcr.2010.10.008
  • Peer Reviewed
• [Journal Article] Biochemical properties of Caenorhabditis elegans HMG-5, a regulator of mitochondrial DNA (2011)
  • Author(s): Sumitani, M, Kasashima, K, Matsugi, J, Endo, H
  • Journal Title: J. Biochem. Volume: 149(5) Issue: 5 Pages: 581-589
  • DOI: 10.1093/jb/mvr008
  • NAID: 10030585363
  • Peer Reviewed
• [Journal Article] Biochemical properties of Caenorhabditis elegans HMG-5, a regulator of mitochondrial DNA (2011)
  • Author(s): 遠藤仁司
  • Journal Title: J Biochem Volume: 149 Pages: 581-589
  • NAID: 10030585363
  • Peer Reviewed
• [Journal Article] Human mitochondrial transcription factor A is required for the segregation of mitochondrial DNA in cultured cells (2011)
  • Author(s): 遠藤仁司
  • Journal Title: Exp.Cell Res. Volume: 317 Pages: 210-220
  • Peer Reviewed
• [Journal Article] Biochemical properties of Caenorhabditis elegans HMG-5, a regulator of mitochondrial DNA. (2011)
  • Author(s): 遠藤仁司
  • Journal Title: J.Biochem. Volume: (Epub)
  • NAID: 10030585363
  • Peer Reviewed
• [Journal Article] SR and SR-related proteins redistribute to segregated fibrillar components of nucleoli in a response to DNA damage. (2010)
  • Author(s): Sakashita, E. and Endo, H.
  • Journal Title: Nucleus Volume: 1(4) Issue: 4 Pages: 367-380
  • DOI: 10.4161/nucl.1.4.12683
  • Peer Reviewed
• [Journal Article] SR and SR-related proteins redistribute to segregated fibrillar components of nucleoli in a response to DNA damage. (2010)
  • Author(s): 遠藤仁司
  • Journal Title: Nucleus Volume: 1 Pages: 367-387
  • Peer Reviewed
• [Journal Article] Human mitochondrial transcription factor A is required for the segregation of mitochondrial DNA in cultured cells. (2010)
  • Author(s): 遠藤仁司
  • Journal Title: Exp.Cell Res. Volume: 317 Pages: 210-220
  • Peer Reviewed
• [Presentation] 紫外線照射により核内再分配されるSR蛋白質の動態解析 (2012)
  • Author(s): 坂下英司、遠藤仁司
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡
• [Presentation] Human ClpX regulates the distribution of mitochondrial DNA through TFAM function (2012)
  • Author(s): 笠嶋克巳、遠藤仁司
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
• [Presentation] ミトコンドリアシャペロンClpXによるTFAM調節機構の解析 (2012)
  • Author(s): 笠嶋克巳、遠藤仁司
  • Organizer: 第12回日本ミトコンドリア学会年会
  • Place of Presentation: つくば
• [Presentation] Thy-1による神経突起伸展およびT細胞凝集を調節するモノクローナル抗体のエピトープ同定 (2012)
  • Author(s): 黒岩憲二、中島充博、遠藤仁司、新井孝夫
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
• [Presentation] 生殖細胞とミトコンドリア (2012)
  • Author(s): 遠藤仁司、長尾恭光 、笠嶋克巳
  • Organizer: 第30回日本受精着床学会
  • Place of Presentation: 大阪
• [Presentation] 生殖細胞とミトコンドリアヌクレオイド (2012)
  • Author(s): 遠藤仁司
  • Organizer: 第３０回日本受精着床学会
  • Place of Presentation: 大阪
  • Invited
• [Presentation] Mitochondrial function of AAA+ protein ClpX as a mitochondrial nucleoid factor (2011)
  • Author(s): Kasashima, K., Sumitani, M., Endo, H.
  • Organizer: The 8th Conference of Asian Society for Mitochondrial Research and Medicine and The 11th Conference of Japanese Society of Mitochondrial Research and Medicine
  • Place of Presentation: Kagoshima
• [Presentation] Oligomeric formation of human TFAM, a diverse regulator of mitochondrial DNA (2011)
  • Author(s): Kasashima, K., Endo, H.
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜
• [Presentation] 加齢性難聴モデルマウスを用いた内耳蝸牛プロテオーム解析 (2011)
  • Author(s): 中村謙一 、太田恵理子 、黒岩憲二 、遠藤仁司
  • Organizer: 日本プロテオーム学会年会
  • Place of Presentation: 新潟
• [Presentation] 内耳蝸牛プロテオーム解析による加齢性難聴関連タンパク質の同定 (2011)
  • Author(s): 中村謙一 、太田恵理子 、黒岩憲二 、遠藤仁司
  • Organizer: 日本生化学会年会
  • Place of Presentation: 京都
• [Presentation] DNA損傷応答によりSR タンパク質は核小体繊維状部へ移行する (2010)
  • Author(s): 坂下英司、遠藤仁司
  • Organizer: 第12回RNA Meeting
  • Place of Presentation: 東京
• [Presentation] CUGBP1 on pyrimidine tract induces exon skipping by preventing exon recognition complex formation of the alternative exon on F1γ pre-mRNA (2010)
  • Author(s): 坂下英司、遠藤仁司
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会大会 合同大会
  • Place of Presentation: 神戸
• [Presentation] Thy-1による神経突起伸展およびT細胞凝集の分子機構 (2010)
  • Author(s): 黒岩憲二、大澤麻文、太田恵理子、遠藤仁司、新井孝夫
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 神戸
• [Presentation] Human TFAM is required for the symmetric segregation of mitochondrial DNA in cultured cells (2010)
  • Author(s): Kasashima, K., Sumitani, M., Endo, H.
  • Organizer: The 7th Conference of Asian Society for Mitochondrial Research and Medicine and The 10th Conference of Japanese Society of Mitochondrial Research and Medicine
  • Place of Presentation: Fukuoka
• [Book] ミトコンドリア膜の機能的新タンパク質類-プロヒビチン、・生体の科学 (2012)
  • Author(s): 遠藤仁司、笠 嶋克巳
  • Publisher: 医学書院
  • URL: http://www.bitway.ne.jp/ejournal/biglobe/2425101344.html
• [Patent(Industrial Property Rights)] ミトコンドリア膜タンパク質群およびそれらをコードする遺伝子群 (2012)
  • Inventor(s): 遠藤仁司
  • Industrial Property Rights Holder: 学校法人自治医科大学
  • Acquisition Date: 2012-02-10
• [Patent(Industrial Property Rights)] プロヒビチン2(PHB2)のミトコンドリア機能 (2012)
  • Inventor(s): 遠藤仁司・笠嶋克巳
  • Industrial Property Rights Holder: 学校法人自治医科大学
  • Acquisition Date: 2012-03-13
• [Patent(Industrial Property Rights)] Mitochondrial function of prohibitin 2 (PHB2). (2012)
  • Inventor(s): 遠藤仁司・笠嶋克巳
  • Industrial Property Rights Holder: 学校法人自治医科大学
  • Acquisition Date: 2012-04-10
  • Overseas
• [Patent(Industrial Property Rights)] プロヒビチン2とPGC1αとの結合を用いた脂肪分化調節剤のスクリーニング方法 (2010)
  • Inventor(s): 遠藤仁司
  • Industrial Property Rights Holder: 学校法人自治医科大学
  • Industrial Property Number: 2010-248139
  • Filing Date: 2010-11-05