Study on histopathological biomarker in cervical lesions based on HPV subtypes and integration status of HPV within host genome

• Project/Area Number: 22590306
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Human pathology
• Research Institution: Gunma University
• Principal Investigator: SANO Takaaki 群馬大学, 大学院・医学系研究科, 准教授 (90292581)
• Co-Investigator(Renkei-kenkyūsha): YOSHIDA Tomomi 群馬大学, 医学部保健学科, 助教 (00312893)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: HPV / p16 蛋白 / L1 蛋白 / 免疫染色 / 子宮頸癌 / 異形成 / 腺癌 / 胃型形質 / p16 / Ki-67 / 細胞診 / p16蛋白 / L1蛋白 / 子宮頚部異形成 / 免疫組織化学 / 自己採取検体

Research Abstract

Human papillomavirus (HPV) L1 capsid protein (L1) is associated with the productive phase of HPV infection. We examined the relationship between L1 and p16 expression in cervical intraepithelial neoplasia. A study compared the usefulness of self-collected (SC)materials for cervical cytology and HPV genotyping. These results suggest that a combination of Pap and HPV tests on SC material may provide a diagnostic strategy with high sensitivity and specificity. A study evaluated the usefulness of p16/Ki-67 as a new biomarker in the diagnosis of cervical lesions. The p16/Ki-67 double immunostaining in cytology sample is useful as a surrogate marker of high-grade lesions.

Research Products

• [Journal Article] Cervical Cytology and Human PapillomaVirus Genotyping with Self-Collected and Clinician-Collected Materials: Analysis with Linear Array Assay (2011)
  • Author(s): Yoshida T, Sano T, Takada N, Kanuma T, Inoue H, Itoh T, Yazaki C, Obara M, Fukuda T.
  • Journal Title: Acta cytol Volume: 55 Pages: 106-112
  • Peer Reviewed
• [Journal Article] Usefulness of CINtec PLUS p16INK4a/Ki-67 double-staining in cytological screening of cervical Cancer (2011)
  • Author(s): Yoshida T, Sano T, Kanuma T, Inoue H, Itoh T, Yazaki C, Obara M, Fukuda T
  • Journal Title: Acta cytol Volume: 55 Pages: 413-420
  • Peer Reviewed
• [Journal Article] 福田利夫病理診断に役立つ分子生物学:Human papillomavirus:解説編 (2011)
  • Author(s): 佐野孝昭、吉田朋美
  • Journal Title: 病理と臨床 Volume: 29(臨時増刊号) Pages: 289-292
• [Journal Article] 病理診断に役立つ分子生物学:Human papillomavirus:診断編 (2011)
  • Author(s): 佐野孝昭、吉田朋美、福田利夫
  • Journal Title: 病理と臨床 Volume: 29(臨時増刊号) Pages: 293-297
• [Journal Article] Usefulness of CINtec® PLUS p16/Ki-67 double-staining in cytological screening of cervical cancer (2011)
  • Author(s): Yoshida T, Sano T, et al.
  • Journal Title: Acta cytologica Volume: 55 Issue: 5 Pages: 413
  • DOI: 10.1159/000331047
  • Peer Reviewed
• [Journal Article] 病理診断に役立つ分子生物学:Human papilloma virus:解説編 (2011)
  • Author(s): 佐野孝昭, ほか
  • Journal Title: 病理と臨床 Volume: 28(臨時増刊号) Pages: 289-292
• [Journal Article] 病理診断に役立つ分子生物学:Human papilloma virus:診断編 (2011)
  • Author(s): 佐野孝昭, ほか
  • Journal Title: 病理と臨床 Volume: 28(臨時増刊号) Pages: 293-297
• [Journal Article] Cervical Cytology and Human Papilloma Virus Genotyping with Self-Colected and Clinician-Collected Materials : Analysis with Linear Array Assay (2011)
  • Author(s): YoshidaT, SanoT, et al.
  • Journal Title: Acta cytologica Volume: 155 Pages: 106-112
  • Peer Reviewed
• [Journal Article] Immunohistological analysis of HPV L1 capsid protein and p16 protein in low-grade dysplastic lesions of the uterine cervix (2010)
  • Author(s): Hoshikawa S, Sano T, Yoshida T, Ito H, Fukuda T, Oyama T.
  • Journal Title: Pathol Res Pract Volume: 206 Pages: 816-820
  • Peer Reviewed
• [Journal Article] Immunohistological analysis of HPV L1 capsid protein and p16 protein in low-grade dysplastic lesions of the uterine cervix (2010)
  • Author(s): Hoshikawa S, Sano T, et al.
  • Journal Title: Pathology Research and Practice Volume: 206 Pages: 816-820
  • Peer Reviewed
• [Presentation] 若年者における子宮頸癌細胞診の普及への試み (2011)
  • Author(s): 吉田朋美、佐野孝昭、高田奈美、鹿沼達哉、小原満雄、伊藤晃子、矢崎千秋、井上浩、福田利夫
  • Organizer: 第52回日本臨床細胞学会総会
  • Year and Date: 2011-05-21
• [Presentation] 若年者における子宮頸癌細胞診の普及への試み (2011)
  • Author(s): 吉田朋美、佐野孝昭, ほか
  • Organizer: 第52回日本臨床細胞学会総会
  • Place of Presentation: 福岡
  • Year and Date: 2011-05-21
• [Presentation] 子宮頸部のLow grade dysplasiaにおけるL1 capsid蛋白およびp16蛋白の免疫組織学的検討 (2011)
  • Author(s): 星川里美、佐野孝昭、吉田朋美、伊藤秀明、小山徹也、福田利夫
  • Organizer: 第100回日本病理学会総会
  • Place of Presentation: 横浜
  • Year and Date: 2011-04-29