| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Research Abstract |
Invasion-associated microRNAs in lung adenocarcinoma were identified by microarray analysis of microRNA. The samples were microdissected from invasive and non-invasive parts of small lung adenocarcinomas. Among 143 microRNAs differentially expressed by more than 2-folds, sequential qRT-PCR study validated five microRNAs (miR-21, miR-107, miR-141, miR-146a, miR-451) as invasion-associated microRNAs, regardless of EGFR gene status. We also identified BTG2 gene as the target of mir-146a. As for the stromal cells in invasive part of lung adenocarcinoma, the combination of immunohistochemistry of smooth-muscle actin and in situ hybridization of mir-21 revealed strong expression of miR-21 in myofibroblasts.
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