Invasion-associated microRNA of small lung adenocarcinoma-A detailed study using laser microdissection
| Project/Area Number | 22590307 |
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Human pathology
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| Research Institution | Akita University (2011-2012)
The University of Tokyo (2010) |
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Principal Investigator |
GOTO Akiteru 秋田大学, 大学院・医学系研究科, 教授 (90317090)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed(Fiscal Year 2012)
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| Budget Amount *help |
¥4,550,000 (Direct Cost : ¥3,500,000、Indirect Cost : ¥1,050,000)
Fiscal Year 2012 : ¥1,170,000 (Direct Cost : ¥900,000、Indirect Cost : ¥270,000)
Fiscal Year 2011 : ¥1,430,000 (Direct Cost : ¥1,100,000、Indirect Cost : ¥330,000)
Fiscal Year 2010 : ¥1,950,000 (Direct Cost : ¥1,500,000、Indirect Cost : ¥450,000)
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| Keywords | 呼吸器・縦隔 / 浸潤・転移 / エピジェネティクス / 肺腺癌 / 間質浸潤 / マイクロＲＮＡ / マイクロRNA / マイクロアレイ / EGFR遺伝子 / 癌浸潤 / マイクロダイセクション / EGFR |
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| Research Abstract |
Invasion-associated microRNAs in lung adenocarcinoma were identified by microarray analysis of microRNA. The samples were microdissected from invasive and non-invasive parts of small lung adenocarcinomas. Among 143 microRNAs differentially expressed by more than 2-folds, sequential qRT-PCR study validated five microRNAs (miR-21, miR-107, miR-141, miR-146a, miR-451) as invasion-associated microRNAs, regardless of EGFR gene status. We also identified BTG2 gene as the target of mir-146a. As for the stromal cells in invasive part of lung adenocarcinoma, the combination of immunohistochemistry of smooth-muscle actin and in situ hybridization of mir-21 revealed strong expression of miR-21 in myofibroblasts.
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Report
(4results)
Research Products
(29results)
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[Journal Article] Sperm-associated antigen 4, a novel hypoxia-inducible factor 1 target, regulates eytokinesis, and its expression correlates with the prognosis of renal cell earcinoma.2013
Author(s)
Shoji K, Mimura I, Murayama T, Wada T, Kume H, Goto A, Ohse T, Tanaka T, Inagi R, van der Hoorn FA, Manabe I, Homma Y, Fukayama M, Sakurai T, Hasegawa T, Aburatani H, Kodama T, Nangaku M.
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Journal Title
American Journal of Pathology
Volume : 182
Pages : 2191-2203
DOI
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Peer Reviewed
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[Journal Article] High-grade Lung Adenocarcinoma With Fetal Lung-like Morphology: Clinicopathologic, Immunohistochemical, and Molecular Analyses of 17 Cases2013
Author(s)
Morita S, Yoshida A, Goto A, Ota S, Tsuta K, Yokozawa K, Asamura H, Nakajima J, Takai D, Mori M, Oka T, Tamaru J, Itoyama S, Furuta K, Fukayama M, Tsuda H.
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Journal Title
Am J Surg Pathol
Volume : 37(6)
Pages : 924-932
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Peer Reviewed
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[Journal Article] Pulmonary Venous Invasion, Determined by Chest Computed Tomographic Scan, as a Potential Early Indicator of Zygomycosis Infection: A Case Series2012
Author(s)
Kitagawa H, Watanabe K, Kage H, Inoh S, Goto A, Fukayama M, Nagase T, Ohishi N, Takai D
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Journal Title
J Thorac Imaging
Volume : 27
DOI
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Peer Reviewed
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[Journal Article] Quantitative analysis of viral load per haploid genome revealed the different biological features of merkel cell polyomavirus infection in skin tumor2012
Author(s)
Ota, S., Ishikawa, S., Takazawa, Y., Goto, A., Fujii, T., Ohashi, K., Fukayama, M
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DOI
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[Journal Article] Harris CC. Macrophages, nitric oxide and microRNAs are associated with DNA damage response pathway and senescence in inflammatory bowel disease2012
Author(s)
Sohn J, Schetter A, Yfantis H, Ridnour L, Horikawa I., Khan M, RoblesA, Hussain S, Goto, A., Bowman E, Hofseth L, Bartkova J, Bartek J, Wogan G, Wink D
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DOI
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[Journal Article] Aberrant expression of tumor suppressors, CADM1 and 4.1B, in invasive lesions of primary breast cancer2012
Author(s)
Takahashi Y, Iwai M, Kawai T, Arakawa A, Ito T, Sakurai-Yageta M, Ito A, Goto A, Saito M, Kasumi F, and Murakami Y.
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Journal Title
Breast Cancer
Volume : 19
Pages : 242-252
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Peer Reviewed
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[Journal Article] CpG island methylation of microRNAs is associated with tumor size and recurrence of non-small-cell lung cancer2011
Author(s)
Kitano K, Watanabe K, Emoto N, Kage H, Hamano E, Nagase T, Sano A, Murakawa T, Nakajima J, Goto A, Fukayama M, Yatomi Y, Ohishi N, Takai D.
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Journal Title
Cancer Sci
Volume : 102
Pages : 2126-2131
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[Journal Article] (*: Equal contribution): MicroRNA expression and clinical outcomes in patients treated with adjuvant chemotherapy after complete resection of nonsmall cell lung carcinoma2010
Author(s)
Voortman J*, Goto A*, Mendiboure J, Sohn JJ, AJ Schetter, Saito M, Dunant A, Pham TC, Petrini I, Lee A, Khan MA, Hainaut P, Pignon JP, Brambilla E, Popper HH, Filipits M, Harris CC, G Giaccone
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Journal Title
Cancer Res
Volume : 70(21)
Pages : 8288-98
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Peer Reviewed
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