| Project/Area Number |
22590321
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Human pathology
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| Research Institution | Toho University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
ISHIKAWA Yukio 東邦大学, 医学部, 准教授 (30276894)
ONO Ichiro 札幌医科大学, 医学部, 准教授 (20125298)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 創傷治癒 / 間葉系前駆細胞 / 組織修復 / 瘢痕 / Fibrocyte / 細小動静脈 / 肉芽組織 / ケロイド / 肥厚性瘢痕 / 蛍光多重染色 |
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| Research Abstract |
In order to investigate the distributional relationship between circulating and resident fibrocytes in relation to vessel formation, double-staining was performed in 49 human skin tissues samples, with CD34 or leukocyte-specific protein-1 (LSP-1) plus pro-collagen I. A considerable difference in the numbers of fibrocytes between extra- and intra-vascular regions was evident, with obviously fewer circulating, than resident fibrocytes. The expression of resident, but not of circulating fibrocytes, changed as healing advanced, suggesting that the different cellular properties of fibrocytes between extra- and intra-vascular regions. The specific distribution of pro-collagen I+/CD34+ circulating fibrocytes was evident in arterioles/venules, with the obviously increased expression of CXCL12 in the endothelial cells. Based on the relatively specific distribution of circulating fibrocytes within arterioles/venules, CXCL12-positive arterioles might provide a microenvironment that promotes CXCR4-mediated fibrocyte chemotaxis.
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