High-throughput tumor-related gene expression analysis in various cell lines.

• Project/Area Number: 22590334
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Human pathology
• Research Institution: Yamaguchi University
• Principal Investigator: KONDO Tomoko 山口大学, 大学院・医学系研究科, 助教 (30379979)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2012: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2010: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: イメージサイトメトリー / 腫瘍マーカー / セルアレイ / 網羅的解析 / サイトメトリー

Research Abstract

We analyzed expression level of tumor makers in many cell lines by ‘cell array system’ which we developed for high content analysis. The expression level of 24 kinds of intracellular molecules was measured in various cell lines by Laser scanning cytometer (LSC). We tried novel classification of tumors based on the data we obtained, but we did not succeed.

Research Products

• [Journal Article] DNA copy number aberrations associated with lymphovascular invasion in upper urinary tract urothelial carcinoma. (2012)
  • Author(s): Misumi T
  • Journal Title: Cancer Genet Volume: 205 Issue: 6 Pages: 313-318
  • DOI: 10.1016/j.cancergen.2012.04.008
  • Peer Reviewed
• [Journal Article] Luminal A and luminal B (HER2 negative) subtypes of breast cancer consist of a mixture of tumors with different genotype. (2012)
  • Author(s): Yanagawa M
  • Journal Title: BMC Res Notes Volume: 25 Issue: 1 Pages: 376-381
  • DOI: 10.1186/1756-0500-5-376
  • Peer Reviewed
• [Journal Article] DNA copy number aberrations associated with the clinicopathological features of colorectal cancers : Identification of genomic biomarkers by array-based comparative genomic hybridization (2011)
  • Author(s): Nakao M, Kawauchi S, Uchiyama T, Adachi J, Ito H, Chochi Y, Furuya T, Oga A, Sasaki K
  • Journal Title: Oncol Rep Volume: 25(6) Pages: 1603-1611
  • DOI: 10.3892/or.2011.1246
  • Peer Reviewed
• [Journal Article] A simple method for enhancing hybridization efficiency in chromosome and array comparative genomic hybridization (2011)
  • Author(s): Kawauchi S, Furuya T, Nakao M, Ikemoto K, Oga A, Sasaki K
  • Journal Title: Biotech Histochem Volume: 86 Issue: 3 Pages: 192-198
  • DOI: 10.3109/10520291003666781
  • Peer Reviewed
• [Journal Article] DNA copy number aberrations associated with the clinicopathological features of colorectal cancers: Identification of genomic biomarkers by array-based comparative genomic hybridization (2011)
  • Author(s): Nakao M, Kawauchi S, Uchiyama T, Acachi J, Ito H, Chochi Y, Furuya T, Oga A, Sasaki K
  • Journal Title: Oncology report Volume: 25 Pages: 1603-1611
  • Peer Reviewed
• [Journal Article] Centrosome amplification as a putative prognostic biomarker for the classification of urothelial carcinomas (2011)
  • Author(s): Yamamoto Y, Misumi T, Eguchi S, Chochi Y, Kitahara S, Nakao M, Nagao K, Hara T, Sakano S, Furuya T, Oga A, Kawauchi S, Sasaki K, Matsuyama H
  • Journal Title: Hum Pahol Volume: 42 Issue: 12 Pages: 1923-1930
  • DOI: 10.1016/j.humpath.2011.02.013
  • Peer Reviewed
• [Journal Article] Loss of 6q or 8p23 is associated with the total number of DNA copy number aberrations in adenoid cystic carcinoma (2011)
  • Author(s): Oga A, Uchida K, Nakao M, Kawauchi S, Furuya T, Chochi Y, Ikemoto K, Okada T, Ueyama Y, Sasaki K, Yousefpour F
  • Journal Title: Oncol Rep Volume: 26(6) Pages: 1393-1398
  • DOI: 10.3892/or.2011.1446
  • Peer Reviewed
• [Journal Article] DNA copy number aberrations associated with the clinicopathological features of colorectal cancers : Identification of genomic biomarkers by array-based comparative genomic hybridization. (2011)
  • Author(s): Nakao M
  • Journal Title: Oncol Rep. Volume: 25(6) Pages: 1603-1611
  • Peer Reviewed
• [Journal Article] DNA copy number aberrations associated with aneuploidy and chromosomal instability in breast cancers (2010)
  • Author(s): Kawauchi S, Furuya T, Ikemoto K, Nakao M, Yamamoto S, Oka M, Sasaki K
  • Journal Title: Oncol Rep Volume: 24 Pages: 875-883
  • Peer Reviewed
• [Journal Article] Loss of BCL2L10 protein expression as prognostic predictor for poor clinical outcome in gastric carcinoma (2010)
  • Author(s): Xu JD, Furuya T, Cao XX, Liu XL, Li QQ, Wang WJ, Xu ZD, Sasaki K, Liu XP
  • Journal Title: Histopathology Volume: 57 Pages: 814-824
  • Peer Reviewed
• [Journal Article] Loss of BCL2L10 protein expression as prognostic predictor for poor clinical outcome in gastric carcinoma. (2010)
  • Author(s): Xu JD.
  • Journal Title: Histopathology Volume: 57(6) Pages: 1365-2559
  • Peer Reviewed
• [Journal Article] DNA copy number aberrations associated with aneuploidy and chromosomal instability in breast cancers. (2010)
  • Author(s): Kawauchi S
  • Journal Title: Onco Rep. Volume: 24(4) Pages: 875-883
  • Peer Reviewed
• [Presentation] 各種乳癌培養細胞に対する 12q15 領域にコードされる遺伝子産物の影響 (2012)
  • Author(s): 古屋智子、伊藤秀明、帖地康世、小賀厚徳、河内茂人、佐々木功典
  • Organizer: 第101回日本病理学会総会
  • Place of Presentation: 東京、京王プラザホテル
  • Year and Date: 2012-04-27
• [Presentation] 各種乳癌培養細胞に対する12q15領域にコードされる遺伝子産物の影響 (2012)
  • Author(s): 古屋智子
  • Organizer: 第101回日本病理学会総会
  • Place of Presentation: 京王プラザホテル（東京）
• [Presentation] Detection fo tetrasomic cells with proliferative capability arising from disomic cells by image cytometry and FISH (2012)
  • Author(s): Hideaki Ito
  • Organizer: Cyto 2012
  • Place of Presentation: Leipzig, Germany
• [Presentation] The evaluation of DNA ploidy and chromosomal changes by image cytometry in bone-marrow mesenchymal stem cells clutire in vitro (2012)
  • Author(s): Tomoko Furuya
  • Organizer: Cyto 2012
  • Place of Presentation: Leipzig, Germany
• [Presentation] 乳癌におけるDNAコピー数異常領域に存在する遺伝子産物の乳癌培養細胞に対する影響についての検討 (2011)
  • Author(s): 古屋智子、河内茂人、帖地康世、伊藤秀明、小賀厚徳、佐々木功典
  • Organizer: 第100回日本病理学会総会
  • Place of Presentation: 横浜、パシフィコ横浜
  • Year and Date: 2011-04-29
• [Presentation] 乳癌におけるDNAコピー数異常領域に存在する遺伝子産物の乳癌培養細胞に対する影響についての検討 (2011)
  • Author(s): 古屋智子
  • Organizer: 第100回日本病理学会総会
  • Place of Presentation: パシフィコ横浜(横浜市)
  • Year and Date: 2011-04-29
• [Presentation] 乳癌のゲノムアレイ解析 (2010)
  • Author(s): 古屋智子
  • Organizer: 第20回日本サイトメトリー学会
  • Place of Presentation: 東京・東京慈恵会医科大学
  • Year and Date: 2010-06-26
• [Presentation] 乳癌におけるDNAコピー数異常と各種マーカーの発現との関連について (2010)
  • Author(s): 古屋智子、河内茂人、帖地康世、小賀厚徳、佐々木功典
  • Organizer: 第99回日本病理学会総会
  • Place of Presentation: 東京、京王プラザホテル
  • Year and Date: 2010-04-29