| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Research Abstract |
We found that occludin, a transmembrane protein found in tightjunctions, had an intra-molecular disulfide bond in its cytoplasmic tail of the c-terminus and that the ratio of disulfide bond formation depended on its sub-cellular location. In addition, we discovered that a disulfide bond in the extracellular loop of occludin might affect its stability. Co-immunoprecipitation with occludin containing a cysteine mutation revealed an associated with some kinds of protein thatmight regulate disulfide bond formation. We also found that zo-1, another tight junctionprotein, might have dimerized via a disulfide bond.
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