The analyses of tight junction formation via disulfide bond
Project/Area Number |
22590336
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Human pathology
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Research Institution | Sapporo Medical University |
Principal Investigator |
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Project Period (FY) |
2010 – 2012
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Project Status |
Completed (Fiscal Year 2012)
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Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
|
Keywords | 分子病理 / タイト結合 / ジスルフィド結合 / thioredoxin / redox / 酸化ストレス |
Research Abstract |
We found that occludin, a transmembrane protein found in tightjunctions, had an intra-molecular disulfide bond in its cytoplasmic tail of the c-terminus and that the ratio of disulfide bond formation depended on its sub-cellular location. In addition, we discovered that a disulfide bond in the extracellular loop of occludin might affect its stability. Co-immunoprecipitation with occludin containing a cysteine mutation revealed an associated with some kinds of protein thatmight regulate disulfide bond formation. We also found that zo-1, another tight junctionprotein, might have dimerized via a disulfide bond.
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Report
(4 results)
Research Products
(32 results)
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[Journal Article] An NF-・B signaling pathway via PKC・regulates replication of respiratory syncytial virus in polarized normal human nasal epithelial cells2011
Author(s)
Masaki T, Kojima T, Okabayashi T, Ogasawara N, Ohkuni T, Obata K, Takasawa A, Murata M, Tanaka S, Hirakawa S, Fuchimoto J, Ninomiya T, Fujii N, Tsutsumi H, Himi T, Sawada N
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Journal Title
Mol Biol Cell
Volume: 22(13)
Issue: 13
Pages: 2144-2156
DOI
Related Report
Peer Reviewed
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[Journal Article] Transcriptional regulation of claudin-18 via specific protein kinase C signaling pathways and modification of DNA methylation in human pancreatic cancaer cells2011
Author(s)
Ito T, Kojima T, Yamaguchi H, Kyuno D, Kimura Y, Imamura M, Takasawa A, Murata M, Tanaka S, Hirata K, Sawada N
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Journal Title
J Cell Biochem
Volume: 112(7)
Issue: 7
Pages: 1761-72
DOI
Related Report
Peer Reviewed
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[Journal Article] Poly(I:C) reduces expression of JAM-A and induces secretion of IL-8 and TNF-・via distinct NF-・B pathways in human nasal epithelial cells2011
Author(s)
Ohkuni T, Kojima T , Ogasawara N, Masaki T, Fuchimoto J, Kamekura R, Koizumi J, Ichimiya S, Murata M, Tanaka S, Himi T, Sawada N
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Journal Title
Toxicology and Applied Pharmacology
Volume: 250
Issue: 1
Pages: 29-38
DOI
Related Report
Peer Reviewed
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[Journal Article] Transcriptional regulation of claudin-18 via specific protein kinase Csignaling pathway and modification of DNA-methylation in human pancreatic cancer cells2011
Author(s)
Ito T, Kojima T, Yamaguchi H, Kyuno D, Kimura Y, Imamura M, Takasawa A, Murata M, Tanaka S, Hirata K, Sawada N
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Journal Title
J Cell Biochem
Volume: 112(7)
Pages: 1761-1772
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Peer Reviewed
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[Journal Article] Transcriptional control of tight junction proteins via a protein kinase C signal pathway in human telomerase reverse transcriptase-transfected human pancreatic duct epithelial cells2010
Author(s)
Yamaguchi H, Kojima T, Ito T, Kimura Y, Imamura M, Son S, Koizumi J, Murata M, Nagayama M, Nobuoka T,Tanaka S,Hirata K, Sawada N
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Journal Title
Am J Pathol
Volume: 177
Issue: 2
Pages: 698-712
DOI
Related Report
Peer Reviewed
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[Journal Article] PPARγ agonists upregulate the barrier function of tight junctions via a PKC pathway in human nasal epithelial cells2010
Author(s)
Ogasawara N, Kojima T, Go M, Ohkuni T, Koizumi J, Kamekura R, Masaki T, Murata M, Tanaka S,Fuchimoto J, Himi T, Sawada N
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Journal Title
Pharmacol Res
Volume: 61
Issue: 6
Pages: 489-498
DOI
Related Report
Peer Reviewed
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[Journal Article] Protein kinase Calpha inhibitor protects against downregulation of claudin-1 during epithelial-mesenchymal transition of pancreatic cancer
Author(s)
Kyuno D, Kojima T, Yamaguchi H, Ito T, Kimura Y, Imamura M, Takasawa A, Murata M, Tanaka S, Hirata K, Sawada N
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Journal Title
Related Report
Peer Reviewed
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