| Project/Area Number |
22590337
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Human pathology
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| Research Institution | Iwate Medical University |
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Principal Investigator |
OIKAWA Hiroki 岩手医科大学, 医学部, 講師 (50285582)
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| Co-Investigator(Kenkyū-buntansha) |
MASUDA Tomoyuki 岩手医科大学, 医学部, 教授 (10199698)
前沢 千早 岩手医科大学, 医学部, 教授 (10326647)
稲葉 亨 岩手医科大学, 医学部, 助教 (60405800)
小谷 康慈 岩手医科大学, 医学部, 助教 (50433488)
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| Co-Investigator(Renkei-kenkyūsha) |
MAESAWA Chihaya 岩手医科大学, 医学部, 教授 (10326647)
INABA Toru 岩手医科大学, 医学部, 助教 (60405800)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2012: ¥130,000 (Direct Cost: ¥100,000、Indirect Cost: ¥30,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2010: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | HSP27 / アセチル化 / βcatenin / 乳癌 / β-catenin / アポトーシス / 細胞増殖 |
|---|
| Research Abstract |
Using silencing of HDAC6 or NACC1 on MB-231, hyperacetylation of HSP27 was seen by immunoprecipitation of the endogenous proteins with anti-HSP27 antibody, but not with anti-acetyl-lysine antibody. None of serial mutations from lysine to arginine in HSP27 also led to evident change of the acetylation state by treatment with HDAC6 siRNA or TSA (HDAC inhibitor). Additionally, LC tandem mass spectorometric analysis did not detect acetylation sites of HSP27 on treatment with HDAC6 siRNA or TSA. Therefore association between HSP27 and β-catenin was examined. Immunohistochemical analysis indicated colocalization of HSP27 and β-catenin in the cytoplasm of MB-231. The interaction between HSP27 and β-catenin was confirmed by immunoprecipitation using cell lysate of MB-231and pull down assay using Halo- or Flag-tagged proteins of HSP27 and β-catenin by wheat germ protein expression system . Silencing of HSP27 induced the ubiquitination ofβ-catenin and the degradation by proteasome system.
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