Biological implication of microRNAs in tumorigenesis, tumor development, and progression ? analysis with in situ hybridization using histopathological sections
| Project/Area Number |
22590339
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Human pathology
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| Research Institution | Fujita Health University |
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Principal Investigator |
INADA Kenichi 藤田保健衛生大学, 医学部, 准教授 (70246081)
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| Co-Investigator(Kenkyū-buntansha) |
水谷 泰嘉 藤田保健衛生大学, 医学部, 助教 (10546229)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2011: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2010: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
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| Keywords | microRNA / in situ hybridization / LNA/DNA オリゴプローブ / thyramide / 病理組織切片 / 食道扁平上皮癌 / Brm / Egr1 / miRNA199a / LNAプローブ / 免疫組織化学 / 胃癌 / C型肝硬変 / C型肝細胞癌 / microRNA199a / LNA/DNAオリゴプローブ / tyramide |
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| Research Abstract |
Although we examined various tumors including esophageal cancer (squamous cell carcinoma), gastric cancer (adenocarcinoma), ovarian cancer (adenocarcinoma), and hepatocellular carcinoma in this study, no apparent biological interrelationship between microRNAs and their targeted specific proteins was revealed except esophageal cancer. We supposed that the central reason for the failure to detect the interrelationship was deterioration of ISH probes and unexpected change of company that supplied them. On the contrary, we found that microRNA 199a, Brm, and Egr1 constructed double-negative feed in esophageal cancer, suggesting that they were mutually related in the process of esophageal carcinogenesis, cancer development, and its progression. But no special relationship was found between them and tumor stage. We are continuously studying about other tumors both epithelial and nonepithelial and various precancerous lesions because this research method is very unique.
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Report
(4 results)
Research Products
(12 results)
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[Journal Article] Novel approach to identifying autoantibodies in rheumatoid synovitis with a biotinylated human autoantigen library and the enzyme-labeled method.2012
Author(s)
Mizutani Y., Matsuoka K., Takeda H.,Shiogama K., Inada K., Hayakawa K.,Yamada H., Miyazaki T., Sawasaki T.,Endo Y., Tsutsumi Y.
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Journal Title
J. Immunol. Method.
Volume: 387
Pages: 57-70
Related Report
Peer Reviewed
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[Journal Article] Rebamipide induces dendritic cell recruitment to N-methyl-N'-nitro-N-nitrosoguanidine (MNNG)-exposed rat gastric mucosa based on IL-1β upregulation.2012
Author(s)
Yamamichi N., Oka M., Inada K., Konno-Shimizu M., Kageyama-Yahara N., Tamai H., Kato J., Fujishiro M., Kodashima S., Niimi K., Ono S.,Tsutsumi Y., Ichinose M., Koike K.
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Journal Title
Biochem. Biophys. Res. Commun
Volume: 424
Pages: 124-129
Related Report
Peer Reviewed
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[Journal Article] Specific in situ visualization of plasma cells producing antibodies against porphyromonasgingivalis in gingival radicular cyst.Application of the enzyme-labeled antigen method.2011
Author(s)
Tsuge S., Mizutani Y., Matsuoka K., Sawasaki T., Endo Y., Naruishi K., Maeda H., Takashiba S., Shiogama K., Inada K., Tsutsumi Y.
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Journal Title
J. Histochem. Cytochem
Volume: 59
Pages: 673-689
Related Report
Peer Reviewed
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[Journal Article] MicroRNAs miR-199a-5p and -3p target the Brm subunit of SWI/SNF to generate a double-negative feedback loop in a variety of human cancers.2011
Author(s)
Sakurai K., Furukawa C., Haraguchi T., Inada K., Shiogama K., Tagawa T., Fujita S., Ueno Y., Ogata A., Ito M., Tsutsumi Y., Iba H.
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Journal Title
Cancer Res
Volume: 71
Pages: 1680-1689
Related Report
Peer Reviewed
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