Analyses of influence by Warburg Effect small molecular Metbolites via Cancer-Stroma interactions
Project/Area Number |
22590342
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Human pathology
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Research Institution | National Center of Neurology and Psychiatry |
Principal Investigator |
FUJII GEN 独立行政法人国立がん研究センター, 研究所, 主任研究員 (90321877)
|
Project Period (FY) |
2010-04-01 – 2014-03-31
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Project Status |
Completed (Fiscal Year 2013)
|
Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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Keywords | がん間質相互作用 / ワールブルグ効果 / 遺伝子発現 / 乳酸 / ピルビン酸 / ラミニン5 / 共培養 |
Research Abstract |
Local fluctuations of small molecular metabolic products concentration, like lactate or pyruvate produced with Warburg effect, were frequently seen in and around cancer tissue. In this research plan, we investigate molecular influences and mechanism of unique expression due to these metabolites concentration changes, via cancer - stroma interaction, for organization, maintenance, and exacerbation of cancer. Using co-cultured cell model system composed with cancer cell and stroma cell, we successfully perused semi-independent expression profiles of each cell group from integrated DNA chip expression data with novel bio-informatics techniques. Several gene products were extracted, and certified as these expressions were specifically responded to those metabolite concentration changes. Molecular signal transduction pathways were presumed by experiments using reagents showing resembled molecular - structure with small molecular metabolites, inhibitors, and activators for Warburg effect.
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Report
(5 results)
Research Products
(42 results)
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[Journal Article] Sesamol suppresses cyclooxygenase-2 transcriptional activity in colon cancer cells and modifies intestinal polyp development in <i>Apc</i><sup>Min/+</sup> mice2014
Author(s)
Shimizu S, Fujii G, Takahashi M, Nakanaishi R, Komiya M, Shimura M, Noma N, Onuma W, Yano T, Mutoh M.
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Journal Title
Journal of Clinical Biochemistry and Nutrition
Volume: 54
Issue: 2
Pages: 95-101
DOI
NAID
ISSN
0912-0009, 1880-5086
Related Report
Peer Reviewed
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[Journal Article] Diagnostic and prognostic significance of the alternatively spliced ACTN4 variant in high-grade neuroendocrine pulmonary tumours2013
Author(s)
Miyanaga A, Honda K, Tsuta K, Masuda M, Yamaguchi U, Fujii G, Miyamoto A, Shinagawa S, Miura N, Tsuda H, Sakuma T, Asamura H, Gemma A, Yamada T
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Journal Title
Ann Oncol
Volume: 24
Pages: 84-90
Related Report
Peer Reviewed
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[Journal Article] Diagnostic and prognostic significance of the alternatively spliced ACTN4 variant in high-grade neuroendocrine pulmonary tumours.2013
Author(s)
Miyanaga, A., Honda, K., Tsuta, K., Masuda, M., Yamaguchi, U., Fujii, G., Miyamoto, A., Shinagawa, S., Miura, N., Tsuda, H., Sakuma, T.,, Asamura, H., Gemma, A., Yamada, T.
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Journal Title
Ann Oncol.
Volume: 24
Issue: 1
Pages: 84-90
DOI
Related Report
Peer Reviewed
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[Journal Article] Involvement of inflammatory factors in pancreatic carcinogenesis and preventive effects of anti-inflammatory agents.2013
Author(s)
Takahashi, M., Mutoh, M., Ishigamori, R., Fujii, G., Imai, T.
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Journal Title
Semin in Immunopathol,
Volume: 35
Issue: 2
Pages: 203-227
DOI
Related Report
Peer Reviewed
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[Journal Article] Suppressive effect of pioglitazone, a PPAR gamma ligand, on azoxymethane-induced colon aberrant crypt foci in KK-A^y mice2012
Author(s)
Ueno T, Teraoka N, Takasu S, Nakano K, Takahashi M, Yamamoto M, Fujii G, Komiya M, Yanaka A, Keiji Wakabayashi K, Mutoh M
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Journal Title
Asian Pac J Cancer Prev
Volume: 13
Pages: 4067-4073
Related Report
Peer Reviewed
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[Journal Article] Effects of triglyceride on colon epithelial cells during colon carcinogenesis2012
Author(s)
Nakano, K., Yamamoto, M., Takahashi, M., Fujii, G., Hifumi, Y., Shimura, M., Nakanishi, R., Komiya, M., Yanaka, A., Mutoh, M
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Journal Title
Ulcer Res
Volume: 39
Pages: 196-200
NAID
Related Report
Peer Reviewed
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[Journal Article] Synergistic suppression of mice intestinal tumorigenesis by angiotensin II receptor blocker and metformin2012
Author(s)
Hifumi, Y., Fujii, G., Takahashi, M., Yamamoto, M., Nakano, K., Nakanishi, R., Shimura, M., Ishigamori, R., Yanaka, A., Mutoh, M
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Journal Title
Ulcer Res
Volume: 39
Pages: 201-206
NAID
Related Report
Peer Reviewed
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[Journal Article] Suppressive effect of pioglitazone, a PPAR gamma ligand, on azoxymethane-induced colon aberrant crypt foci in KK-Ay mice.2012
Author(s)
Ueno, T., Teraoka, N., Takasu, S., Nakano, K., Takahashi, M., Yamamoto, M., Fujii, G., Komiya, M., Yanaka, A., Wakabayashi, K., Mutoh, M.
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Journal Title
Asian Pac. J. Cancer Prev.
Volume: 13
Issue: 8
Pages: 4067-4073
DOI
Related Report
Peer Reviewed
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[Journal Article] Novel Compound SK-1009 Suppresses Interleukin-6 Expression through Modulation of Activation of Nuclear Factor-KappaB Pathway2012
Author(s)
Shimura, M., Yamamoto, M., Fujii, G., Takahashi, M., Komiya, M., Noma, N., Tanuma, S., Yanaka, A., Mutoh, M.
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Journal Title
Biological and Pharmaceutical Bulletin
Volume: 35
Issue: 12
Pages: 2186-2191
DOI
NAID
ISSN
0918-6158, 1347-5215
Related Report
Peer Reviewed
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