Role of plasma ribosomal protein S19 in cellular immunity

• Project/Area Number: 22590348
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Experimental pathology
• Research Institution: Kumamoto University
• Principal Investigator: SEMBA Umeko 熊本大学, 大学院・生命科学研究部, 助教 (50109691)
• Co-Investigator(Kenkyū-buntansha): YAMAMOTO Tetsuro 熊本大学, 大学院・生命科学研究部, 教授 (60112405)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2011: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2010: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
• Keywords: RP S19 / 補体系 / 結核菌 / 蛋白質 / 生体防御

Research Abstract

When mycobacterial dead cells were exposed to plasma, ribosomalprotein S19 (RP S19) was cross-linked by a transglutaminase-catalyzed reaction on themycobacterium membrane, working as the negatively charged scaffold, during bloodcoagulation. The cross-linked RP S19 dimer induced chemotactic response ofmonocytes/macrophages recruitment via C5a receptor, suggesting phagocytosis ofmycobacterium by recruited macrophages. C5a anaphylatoxin was partly involved in thismonocyte chemotaxis. It was demonstrated that RP S19 participated in innate immunityagainst the mycobacterium infection in association with C5a.

Research Products

• [Journal Article] Inhibitoryeffects of C4a on chemoattractant andsecretagogue functions of the otheranaphylatoxins via Gi protein-adenylylcyclase inhibition pathway in mast cells.Int (2012)
  • Author(s): Xie P, Nishiura H, Semba U, Chen J, ZhaoR, Kuniyasu A, Yamamoto T
  • Journal Title: Immunopharmacol. Jan Volume: 12(1) Issue: 1 Pages: 158-68
  • DOI: 10.1016/j.intimp.2011.11.006
  • Peer Reviewed
• [Journal Article] Ribosomal protein S19 is present in plasma as a complex with prothrombin (2011)
  • Author(s): Nishiura, H., Tanase, S., Tsujita, K., Sugiyama, S., Ogawa, ・H., Nakagaki, T., Semba, U., and Yamamoto, T.
  • Journal Title: Eur. J. Haematol. Volume: 86 Issue: 5 Pages: 436-441
  • DOI: 10.1111/j.1600-0609.2011.01585.x
  • Peer Reviewed
• [Journal Article] Maintenance of ribosomal protein S19 in plasma by complex formation with prothrombin. (2011)
  • Author(s): Hiroshi Nishiura
  • Journal Title: European Journal of Haematology Volume: (in press)
  • Peer Reviewed
• [Journal Article] Base of molecular mimicry between humanribosomal protein S19 dimer and human C5aanaphylatoxin (2010)
  • Author(s): Nishiura H, Chen J, Ota Y, Semba U,Higuchi H, Nakashima T, Yamamoto T
  • Journal Title: Int Immunopharmacol Volume: 10(12) Issue: 12 Pages: 1541-7
  • DOI: 10.1016/j.intimp.2010.09.002
  • NAID: 120003184562
  • Peer Reviewed
• [Journal Article] Base of molecular mimicry between human ribosomal protein S19 dimer and human C5a anaphylatoxin. (2010)
  • Author(s): Hiroshi Nishiura
  • Journal Title: International Immunopharmacology Volume: 10 Pages: 1541-1547
  • Peer Reviewed
• [Presentation] Involvement of cross-linked ribosomalprotein S19 oligomers and C5a receptor indefinitive erythropoiesis (2013)
  • Author(s): Nishiura H, Chen J, Semba U, YamamotoT
  • Organizer: 第38回日本微小循環学会総会
  • Place of Presentation: 東京慈恵会医科大学(東京)
  • Year and Date: 2013-02-09
• [Presentation] Involvement of cross-linked ribosomal protein S19 oligomers and C5a receptor in definitive erythropoiesis (2013)
  • Author(s): Nishiura H
  • Organizer: 第38回日本微小循環学会総会
  • Place of Presentation: 東京慈恵会医科大学
• [Presentation] リボソームタンパク質 S19 架橋化多量体の赤血球造血への関与 (2012)
  • Author(s): 千場梅子、〓俊、西浦弘志、山本哲郎
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡国債会議場 マリンメッセ福岡(福岡)
  • Year and Date: 2012-12-15
• [Presentation] リボソームタンパク質S19架橋化多量体の赤血球造血への関与 (2012)
  • Author(s): 千場 梅子
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: マリンメッセ福岡
• [Presentation] 血漿リボソームタンパク質 S19 の結核菌感染に対する自然免疫における役割 (2011)
  • Author(s): 千場梅子、〓俊、山本哲郎
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 国立京都国際会館(京都)
  • Year and Date: 2011-09-22
• [Presentation] Role ofplasma ribosomal protein S19 and coagulation factor XIIIa in cellular fibrinolysis and innate immunity againstmycobacterial infection (2011)
  • Author(s): Yamamoto T, Semba U, Nishiura H
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 国立京都国際会館(京都)
  • Year and Date: 2011-09-21
• [Presentation] Ribosomal protein S19-prothrombincomplex in plasma and its role in coagulumresorption (2011)
  • Author(s): Semba U, Nishiura H, Yamamoto T
  • Organizer: 第36回日本微小循環学会年次総会
  • Place of Presentation: 名古屋市立大学病院(名古屋)
  • Year and Date: 2011-02-11
• [Presentation] 血漿リボソームタンパク質S19の結核菌感染に対する自然免疫における役割 (2011)
  • Author(s): 千場梅子
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 国立京都国際会館
• [Presentation] Ribosomal protein S19-prothrombin complex in plasma and its role in coagulum resorption. (2011)
  • Author(s): 千場梅子
  • Organizer: 第36回 日本微小循環学会総会
  • Place of Presentation: 名古屋市立大学病院ホール