Significance of triglyceride metabolism in human vascular smooth muscle cell phenotype

• Project/Area Number: 22590349
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Experimental pathology
• Research Institution: University of the Ryukyus
• Principal Investigator: MATSUMOTO Hirofumi 琉球大学, 医学部附属病院, 助教 (90532408)
• Co-Investigator(Kenkyū-buntansha): KATO Seiya 琉球大学, 大学院・医学研究科, 教授 (60268844)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 血管平滑筋細胞 / トリグリセリド / 脂質代謝 / ATGL / 細胞 / 脂肪酸代謝

Research Abstract

VSMC could use TG mediated ATGL as an cellular energy. TG load in VSMC provided TG accumulation and enhanced TG metabolism and associated with inflammatory phenotype. In addition, coordination of ATGL expression level and beta-oxidation level was probably responsible for inflammatory phenotype. We believe that this culture model is useful in resolution of inflammatory mechanism in neointima and relationship between TG metabolism and atherosclerosis.

Research Products

• [Journal Article] Vascular smooth muscle cells isolated from adipose triglyceride lipase-deficient mice exhibit distinct phenotype and phenotypic plasticity (2013)
  • Author(s): Lin Y, Chiba S, Suzuki A, Yamaguchi S,Nakanishi T, Matsumoto H, Ikeda Y, Ishibashi-Ueda H, Hirano K, Kato S
  • Journal Title: Biochem Biophys Res Commun Volume: 434 Pages: 534-40
  • Peer Reviewed
• [Journal Article] 若年者の動脈硬化病変 (2011)
  • Author(s): 加藤誠也, 松本裕文, 仲西貴也
  • Journal Title: Cardiac Practice Volume: 22 Pages: 6-10
• [Presentation] Ectopic gene transfer successfully corrects vascular smooth muscle phenotypes of adipose tissue triglyceride lipase deficient mouse (2012)
  • Author(s): Yanhui Lin, Shunmei Chiba, Ken-ichi Hirano, Akira Suzuki, Satoshi Yamaguchi, Takaya Nakanishi, Hirofumi Matsumoto, Seiya Kato
  • Organizer: American Heart Association Scientific Sessions
  • Place of Presentation: Los Angels, USA
  • Year and Date: 2012-11-04
• [Presentation] オレイン酸添加血管平滑筋培養細胞の飢餓刺激による中性脂肪代謝と細胞形質との関連 (2012)
  • Author(s): 松本裕文, 仲西貴也, 千葉俊明, YanhuiLin, 又吉宣, 平野賢一, 加藤誠也
  • Organizer: 第101回日本病理学会総会
  • Place of Presentation: 東京都新宿区
  • Year and Date: 2012-04-28
• [Presentation] Ectopic gene transfer successfully corrects vascular smooth muscle phenotypes of adipose tissue triglyceride lipase deficient mouse. (2012)
  • Author(s): Yanhui Lin, Shunmei Chiba, Ken-ichi Hirano, Akira Suzuki, Satoshi Yamaguchi, Takaya Nakanishi, Hirofumi Matsumoto, Seiya Kato.
  • Organizer: American Heart Association Scientific Sessions
  • Place of Presentation: アメリカ、ロサンゼルス
• [Presentation] Adipose Tissue Triglyceride Lipase(ATGL)欠損マウスに由来する大動脈平滑筋細胞の形質と野生型ATGL遺伝子導入の効果 (2012)
  • Author(s): 林 艶輝、千葉俊明、鈴木 朗、山口知是、仲西貴也、松本裕文、池田善彦、植田初江、平野賢一、加藤誠也
  • Organizer: 第31回分子病理学研究会
  • Place of Presentation: 岐阜県恵那
• [Presentation] Relationship between lipid deposition and ATGL expression in human myocardial tissue: A study with autopsy cases (2011)
  • Author(s): Takaya Nakanishi, Hirofumi Matsumoto, Yoshihiko Ikeda, Hatsue Ueda, Kenichi Hirano, Seiya Kato
  • Organizer: 第1回中性脂肪蓄積心筋血管症国際シンポジウム
  • Place of Presentation: 京都
  • Year and Date: 2011-11-26
• [Presentation] Relationship between lipid deposition and ATGL expression in human myocardial tissue : A study with autopsy cases (2011)
  • Author(s): Takaya Nakanishi, Hirofumi Matsumoto, Yoshihiko Ikeda, Hatsue Ueda, Kenichi Hirano, Seiya Kato
  • Organizer: 第1回中性脂肪蓄積心筋血管症国際シンポジウム
  • Place of Presentation: 京都府京都市京都大学百周年時計台記念館百周年記念ホール
  • Year and Date: 2011-11-26
• [Presentation] 剖検症例における中性脂肪蓄積心筋血管症の検索と培養心筋細胞におけるATGL の機能解析 (2011)
  • Author(s): 仲西貴也, 松本裕文, 千葉俊明, 池田善彦, 植田初江, 平野賢一, 加藤誠也
  • Organizer: 第100回日本病理学会総会
  • Place of Presentation: 神奈川県横浜市
  • Year and Date: 2011-04-30
• [Presentation] 血管平滑筋細胞の古典的形質転換と炎症性形質転換 (2011)
  • Author(s): 加藤誠也, Lin Yanhui, 松本裕文, 新垣和也, 又吉宣, 仲西貴也, 千葉俊明, 荒川文子, 大島孝一, 洲鎌和茂
  • Organizer: 第100回日本病理学会総会
  • Place of Presentation: 神奈川県横浜市
  • Year and Date: 2011-04-30
• [Presentation] 剖検症例における中性脂肪蓄積心筋血管症の検索と培養心筋細胞におけるATGLの機能解析 (2011)
  • Author(s): 仲西貴也, 松本裕文, 千葉俊明, 池田善彦, 植田初江, 平野賢一, 加藤誠也
  • Organizer: 第100回日本病理学会総会
  • Place of Presentation: 神奈川県横浜市パシフィコ横浜
  • Year and Date: 2011-04-30
• [Presentation] 血管平滑筋細胞の古典的形質転換と炎症性形質転換 (2011)
  • Author(s): 加藤誠也, Lin Yanhui, 松本裕文, 新垣和也, 又吉宣, 仲西貴也, 千葉俊明, 荒川文子, 大島孝一, 洲鎌和茂
  • Organizer: 第100回日本病理学会総会
  • Place of Presentation: 神奈川県横浜市パシフィコ横浜
  • Year and Date: 2011-04-30
• [Presentation] MODIFICATION OF IL-4 MEDIATED VASCULAR CELL-CELL INTERACTION AND PHENOTYPES USING CELL-SELECTIVE MOUSE IL-4 AGONIST (2010)
  • Author(s): Lin YH, Chiba S, Matayoshi, Arakaki K, Matsumoto H, Nakanishi T, Arakawa F, Oshima K, Sugama K, Kato S
  • Organizer: 第56回日本病理学会秋期特別総会International Poster Session
  • Place of Presentation: 福岡県北九州市
  • Year and Date: 2010-11-25