Cellular and microenvironmental factors controlling hepatitis Cvirus infection, examined by micro-proteomics of human liver tissues.
| Project/Area Number |
22590350
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Experimental pathology
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| Research Institution | Nihon University |
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Principal Investigator |
ESUMI Mariko 日本大学, 医学部, 准教授 (10147019)
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| Co-Investigator(Kenkyū-buntansha) |
SUGITANI Masahiko 日本大学, 医学部, 教授 (40187654)
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| Research Collaborator |
YAMAGUCHI Hiromi 日本大学, 医学部, 専修研究員 (90547118)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Keywords | C 型肝炎ウイルス / 感染制御 / 微小環境 / レーザーマイクロダイセクション / プロテオミクス / 細胞膜セリンプロテアーゼ / ゴルジ体 / C型肝炎ウイルス / ウイルス量 / GOLM1 / 細胞膜セリンプロテーゼ / TMPRSS2 / ブロテオミクス / 肝非実質 |
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| Research Abstract |
Hepatitis C virus (HCV) is a major cause of hepatocellular carcinoma. Anti-HCV drugs developed have still some problems such as side effects and drug-resistant mutant viruses. In this study, we investigated a new strategy of humane anti-HCV therapy based on human-made anti-HCV response. We examined which proteins were differentially produced in liver tissues containing different HCV loads by 1000 folds. When we compared them, we obtained liver tissue samples separately from liver parenchyma and stroma by laser microdissection. Ninety and 27 were differential proteins between the two groups of high and low viral loads, from the liver parenchyma and stroma, respectively. Both pro-viral and anti-viral proteins were up-regulated in the high HCV groups. Proteins which function was unknown in the viral infection were examined by the in vitro HCV infection system using cultured cells. We found two proteins up-regulated in the high HCV groups: a serine protease involved in the entry step of HCV infection and a golgi protein related to the secretion of HCV.
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Report
(4 results)
Research Products
(20 results)
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[Journal Article] High TSC22D3 and low GBP1 expression in the liver is a risk factor for early recurrence of hepatocellular carcinoma2011
Author(s)
Takagi K, Takayama T, Nagase H, Moriguchi M, Wang X, Hirayamagi K, Suzuki T, Hasegawa H, Ochiai T, Yamaguchi N, Kochi M, Kimura M, Esumi M
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Journal Title
Experimental and Therapeutic Medicine
Volume: 2
Pages: 425-431
Related Report
Peer Reviewed
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[Presentation] Liver microenvironment containing cytokeratin 19-positive cells associated with the early recurrence of hepatocellular carcinoma2011
Author(s)
Esumi M, Yamaguchi H, Kuroda K, Munekata Y, Obana Y, Takahashi R, Fuchinoue F, Sugitani M, Nagase H, Takemura T, Minowa T, Hanagata N, Nakayama H, Takayama T
Organizer
The 3rd JCA-AACR Special Joint Conference-The Latest Advances in Liver Cancer Research : From Basic Science to Therapeutics
Place of Presentation
舞浜
Year and Date
2011-03-02
Related Report
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