The role of mouse APOBEC3 in the regulation of endogenous retrovirus

• Project/Area Number: 22590352
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Experimental pathology
• Research Institution: Kinki University
• Principal Investigator: KAWAHARA Sachiyo 近畿大学, 医学部, 講師 (60297629)
• Co-Investigator(Kenkyū-buntansha): MIYAZAWA Masaaki 近畿大学, 医学部, 教授 (60167757) ; HAKATA Yoshiyuki 近畿大学, 医学部, 助教 (30344500)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 発がん制御 / 内在性レトロウイルス / APOBEC3 / 乳癌 / リンパ腫 / 白血病

Research Abstract

Mouse APOBEC3, a host factor that restricts the replication of exogenous retrovirus, is abundantly expressed in virus-resistant C57BL/6 (B6) mice. We have found that loss of APOBEC3 causes spontaneous mammary carcinoma and T cell lymphomas in B6 mice. The transcripts of endogenous mouse mammary tumor virus are abundant in the tumor cells prepared from APOBEC3-deficient mice. These results suggest that APOBEC3 contributes to the control of activated endogenous retrovirus.

Research Products

• [Journal Article] Molecular evolution of physiologically functioning anti-retroviral APOBEC3 deaminases (2012)
  • Author(s): Miyazawa M
  • Journal Title: Uirusu Volume: 62 Pages: 27-38
  • NAID: 10030767931
  • URL: http://dx.doi.org/10.2222/jsv.62.27
• [Journal Article] Molecular evolution of physiologically functioning anti-retroviral APOBEC3 deaminases. (2012)
  • Author(s): Miyazawa M. et al.
  • Journal Title: ウイルス Volume: 62 Pages: 27-38
  • NAID: 10030767931
• [Journal Article] In vivo diagnostic imaging using micro-CT: sequential and comparative evaluation of rodent models for hepatic/brain ischemia and stroke. (2012)
  • Author(s): Hayasaka N. et al.
  • Journal Title: PLoS One Volume: 7
  • Peer Reviewed
• [Journal Article] Two genetic determinants acquired late in Mus evolution regulate the inclusion of exon 5, which alters mouse APOBEC3 translation efficiency (2012)
  • Author(s): Li, J., Y. Hakata, E. Takeda, Q. Liu, Y. Iwatani, C. A. Kozak, M. Miyazawa
  • Journal Title: PLoS Pathogens Volume: 8 Issue: 1 Pages: e1002478-e1002478
  • DOI: 10.1371/journal.ppat.1002478
  • NAID: 120006985441
  • Peer Reviewed
• [Journal Article] Two genetic determinants acquired late in mus evolution regulate the inclusion of exon 5,which alters mouse APOBEC3 translation efficiency (2011)
  • Author(s): Li J, Hakata Y, Takeda E, Liu Q, Iwatani Y, Kozak CA, Miyazawa M
  • Journal Title: PLoS Pathog Volume: 8(1)
  • NAID: 120006985441
  • Peer Reviewed
• [Journal Article] A hole in the T-cell repertoire induced after retroviral infection of immunocompetent adult mice (2011)
  • Author(s): Miyazawa, M., S. Takamura, S. Tsuji-Kawahara, E. Kajiwara, T. Chikaishi, and M. Kato
  • Journal Title: Retrovirol Volume: 8 (Suppl. 2)
  • Peer Reviewed
• [Journal Article] Natural killer cells recognize friend retrovirus-infected erythroid progenitor cells through NKG2D-RAE-1 interactions in Vivo (2011)
  • Author(s): Ogawa T*, Tsuji-Kawahara S*, Yuasa T, Kinoshita S, Chikaishi T, Takamura S, Matsumura H, Seya T, Saga T, Miyazawa M
  • Journal Title: J Virol Volume: 85 Pages: 5423-35
  • Peer Reviewed
• [Journal Article] Attenuated Food Anticipatory Activity and Abnormal Circadian Locomotor Rhythms in Rgs16 Knockdown Mice (2011)
  • Author(s): Hayasaka N, Aoki K, Kinoshita S, Yamaguchi S, Wakefield J. K, Tsuji-Kawahara S, Horikawa K, Ikegami H, Wakana S, Murakami T, Ramabhadran R, Miyazawa M, and Shibata S
  • Journal Title: PLoS ONE Volume: 6
  • Peer Reviewed
• [Journal Article] Natural killer cells recognize friend retrovirus-infected erythroid progenitor cells through NKG2D-RAE-1 interactions in vivo (2011)
  • Author(s): Ogawa T., et al
  • Journal Title: J.Virol. Volume: 85 Pages: 5423-35
  • Peer Reviewed
• [Journal Article] Attenuated Food Anticipatory Activity and Abnormal Circadian Locomotor Rhythms in Rgs16 Knockdown Mice. (2011)
  • Author(s): Hayasaka N, Aoki K, Kinoshita S, Yamaguchi S, Wakefield J.K, Tsuji-
  • Journal Title: Plos ONE Volume: 6
  • Peer Reviewed
• [Journal Article] Persistence of viremia and production of neutralizing antibodies differentially regulated by polymorphic APOBEC3 and BAFF-R loci in Friend virus-infected mice (2010)
  • Author(s): Tsuji-Kawahara S, Chikaishi T., Takeda E., Kato M., Kinoshita S., Kajiwara E., Takamura S., and Miyazawa M
  • Journal Title: J. Virol Volume: 84 Pages: 6082-95
  • Peer Reviewed
• [Journal Article] Premature terminal exhaustion of friend virus-specific effector CD8(+) T cells by rapid induction of multiple inhibitory receptors (2010)
  • Author(s): Takamura S, Tsuji-Kawahara S, Yagita H, Akiba H, Sakamoto M, Chikaishi T, Kato, M, Miyazawa M
  • Journal Title: J Immunol Volume: 184 Pages: 4696-707
  • Peer Reviewed
• [Journal Article] Persistence of viremia and production of neutralizing antibodies differentially regulated by polymorphic APOBEC3 and BAFF-R loci in Friend virus-infected mice. (2010)
  • Author(s): Tsuji-Kawahara S, Chikaishi T., Takeda E., Kato M., Kinoshita S., Kajiwara E.
  • Journal Title: J.Virol. Volume: 84 Pages: 6082-6095
  • Peer Reviewed
• [Journal Article] Premature terminal exhaustion of friend virus-specific effector CD8(+) T cells by rapid induction of multiple inhibitory receptors. (2010)
  • Author(s): Takamura S, Tsuji-Kawahara S, Yagita H, Akiba H, Sakamoto M, Chikaishi T, Kato
  • Journal Title: J.Immunol. Volume: 184 Pages: 4696-4707
  • Peer Reviewed
• [Presentation] Rapid production of virus-neutralizing IgM antibodies and protection against lethal retroviral infection in mice deficient of activation-induced cytidine deaminase (AID) (2012)
  • Author(s): Miyazawa M, Tsuji-Kawahara S
  • Organizer: 24th Workshop on Retrovirla Pathogenesis
  • Place of Presentation: Philadelphia PA
  • Year and Date: 2012-10-24
• [Presentation] Vpr依存的G2期停止におけるVprと宿主因子との分子間相互作用 (2012)
  • Author(s): 博多義之
  • Organizer: 第60回日本ウイルス学会
  • Place of Presentation: 大阪市 大阪国際会議場
• [Presentation] Rapid production of virus-neutralizing IgM antibodies and protection against lethal retroviral infection in mice deficient of activation-induced cytidine deaminase (AID) (2012)
  • Author(s): Miyazawa M.
  • Organizer: 24th Workshop on Retrovirla Pathogenesis
  • Place of Presentation: philadelphia, PA
• [Presentation] Production of virus-neutralizing antibodies and protection against lethal retroviral infection in mice deficient of activation-induced (2011)
  • Author(s): Kato M., et al
  • Organizer: 第40回日本免疫学会学術集会
  • Place of Presentation: 千葉市幕張メッセ
  • Year and Date: 2011-11-27
• [Presentation] Infection of thymus with murine retrovirus induces virus-specific central tolerance that prevents dynamic differentiation of functional memory CD8+ T cells (2011)
  • Author(s): Takamura S., et al
  • Organizer: 第40回日本免疫学会学術集会
  • Place of Presentation: 千葉市幕張メッセ
  • Year and Date: 2011-11-27
• [Presentation] Host immune responses detarmine integration of either F-MuLV alone or F-MuLV plus SFFV in Friend virus leukemogenesis (2011)
  • Author(s): Miyazawa M., et al
  • Organizer: The 23rd Workshop on Retroviral pathogenesis
  • Place of Presentation: Monpellier, France
  • Year and Date: 2011-11-02
• [Presentation] Functional consequences of mouse APOBEC3 gene polymorphisms and multiple genetic factors that influence the production of virus-neutralizing antibodies in Friend virus-infected mice (2011)
  • Author(s): Miyazawa M., et al
  • Organizer: The 23rd Workshop on Retroviral pathogenesis
  • Place of Presentation: Monpellier, France
  • Year and Date: 2011-11-02
• [Presentation] A hole in the T-cell repertoire induced after retroviral infection of immunocompetent adult mice (2011)
  • Author(s): Miyazawa M., et al
  • Organizer: Frontiers of Retrovirology
  • Place of Presentation: Amsterdam, The Netherlands
  • Year and Date: 2011-10-03
• [Presentation] A hole in the T-cellrepertoire induced after retroviral infection ofimmunocompetent adult mice (2011)
  • Author(s): Miyazawa, M., Tsuji-Kawahara S
  • Organizer: Frontiers of Retrovirology
  • Place of Presentation: Amsterdam, The Netherlands
• [Presentation] Host immune responses determine integration of either F-MuLV alone or F-MuLV plus SFFV in Friend virus leukemogenesis (2011)
  • Author(s): Miyazawa, M., Tsuji-Kawahara S
  • Organizer: The 23rd Workshop on Retrovirla Pathogenesis
  • Place of Presentation: Montpellier, Farance
• [Presentation] Polymorphisms in mouse APOBEC3 influence the production of virus-neutralizing antibodies by altering the levels of retrovirus-induced B-cell dysfunction (2010)
  • Author(s): Tsuji-Kawahara, S
  • Organizer: 第14回国際免疫学会
  • Place of Presentation: 神戸市
  • Year and Date: 2010-08-26
• [Presentation] Premature terminal exhaustion of Friend virus-specific effector CD8+ T cells by rapid induction of multiple inhibitory receptors (2010)
  • Author(s): Takamura S., Tsuji-Kawahara, S., Miyazawa, M
  • Organizer: 第14回国際免疫学会
  • Place of Presentation: 神戸市
  • Year and Date: 2010-08-26
• [Presentation] Rac2が発揮する抗HIV-1活性の分子機構 (2010)
  • Author(s): Hakata Y
  • Organizer: 第58回日本ウイルス学会
  • Place of Presentation: 徳島市
• [Remarks]
  • URL: http://www.med.kindai.ac.jp/immuno/