Human gene therapy model by induction of stable bone marrow chimerim with regulation of proliferative ability of hematopoietic stem cell
| Project/Area Number |
22590355
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Experimental pathology
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| Research Institution | Kitasato University (2011-2012)
The University of Tokyo (2010) |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
OTSU Makoto 東京大学, 医科学研究所, 准教授 (30361330)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 造血幹細胞 / 遺伝子治療 / Lnk 遺伝子変異体 / 骨髄キメリズム / 遺伝子導入 / 慢性肉芽腫症 / 造血幹細胞移植 / Lnk 蛋白 / 幹細胞増殖能制御 / 移植前処置軽減 / プラム色素マーカー遺伝子 / 遺伝子導入ウイルス / 遺伝子治療モデル / Lnk遺伝子 / 骨髄造血幹前駆細胞移植 / 細胞内アダプター蛋白 |
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| Research Abstract |
The gene therapy using hematopoietic stem cells (HSCs) is a curative for X-linked chronic granulomatous disease. However, long-term treatment result is not satisfactory because of disappearance of neutrophils with functional reconstitution. We attempt the development of curative gene therapy model by transductionof Lnk-gene variant which promotes appropriate proliferation into HSCs. We made an animal gene therapy model with reproducing clinical problem of human gene therapy. But, the confirmation of the efficacy of transduction or expression of Lnk variant is difficult. We reexamine the modification of a gene vector or methods for transduction.
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Report
(4 results)
Research Products
(2 results)
