| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
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| Research Abstract |
We found that the nestin-negative glioma cells were grown around the nestin-positive glioma cells growing in perivascular in the subcutaneous tumors grown as xenografts in nude mice. By using FACS, these two populations were successfully harvested. Global gene expression profiles demonstrated that some mesenchymal markers were up-regulated in the nestin-positive glioma cells. In the prescence of TGF-beta, nestin-positive glioma cells differentiated into myofibroblasts and osetoblasts. We also found that nestin-positive glioma cells were more resistant to anti-cancer reagents compared with nestine-negative glioma cells. Taken together, nestin-positive glioma cells were considered to be more undifferentiated than nestin-negative glioma cells.
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