Establishment of organoid culture for basal-cell like breast cancer and solving the mechanism to maintain the morphology
| Project/Area Number |
22590358
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Experimental pathology
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| Research Institution | Kanazawa Medical University (2011-2012)
Kyoto University (2010) |
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Principal Investigator |
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | がん / FRET / 腺腫 / 極性 / イメージング / 形態 / 低分子量G蛋白質 / MDCK / 増殖 / 細胞周期 / 分裂軸 |
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| Research Abstract |
Using organoid culture, FRET biosensor-based live imaging, and the inducible system which allows manipulating protein expression and localization, I aimed to reveal signal transduction events which regulate normal cell polarity and morphology of the epithelial structures, and malignant situation where the polarity is disrupted. Although in some cases, benign phenotypes such as adenoma or hyperplasia were reconstituted, no malignant phenotypes including invasion were not observed. It should be noted the systems established in this study is useful to dissect a single gene function in the epithelial cells. Using the systems, we are now ready to reconstitute the cancer situation combining several mutations.
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Report
(4 results)
Research Products
(38 results)
