| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Research Abstract |
Using organoid culture, FRET biosensor-based live imaging, and the inducible system which allows manipulating protein expression and localization, I aimed to reveal signal transduction events which regulate normal cell polarity and morphology of the epithelial structures, and malignant situation where the polarity is disrupted. Although in some cases, benign phenotypes such as adenoma or hyperplasia were reconstituted, no malignant phenotypes including invasion were not observed. It should be noted the systems established in this study is useful to dissect a single gene function in the epithelial cells. Using the systems, we are now ready to reconstitute the cancer situation combining several mutations.
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