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Analysis of microRNA function on heart and smooth muscles and itsanti-tumor effect using genetically modified mice

Research Project

Project/Area Number 22590369
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Experimental pathology
Research InstitutionChubu University

Principal Investigator

IWAMOTO Takashi  中部大学, 生命健康科学部, 教授 (60223426)

Co-Investigator(Kenkyū-buntansha) ICHIHARA Masatoshi  中部大学, 生命健康科学部, 教授 (00314013)
UEYAMA Tomomi  京都府立医科大学, 医学系研究科, 講師 (80379388)
NAKAYAMA Shinsuke  名古屋大学, 医学系研究科, 准教授 (30192230)
Co-Investigator(Renkei-kenkyūsha) OUCHI Yasuo  中部大学, 実験動物教育研究センター, 助教 (70553858)
Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywordsマイクロ RNA / トランスジェニックマウス / 癌 / 拡張型心筋症 / マイクロRNA / アンジオテンシン変換酵素 / マイクロRNA / 大腸癌 / c-Myc / p68/p72 / 心不全 / 心筋細胞
Research Abstract

Although accumulating evidence has shown that microRNAs are involved in many human diseases, the molecular mechanisms are still unknown. In this project, we reveal that strong expression of certain microRNAs suppresses the intestine tumor development in the model mice of familial adenomatous polyposis and that cooperation of miRNAs plays pivotal roles in inhibiting protooncogene expression. We further show that key components for biogenesis of a subset of tumor suppressor microRNAs are downregulated in the transgenic tumors. These data indicate the complicated feedback inhibition between micoRNAs and molecules for their biogenesis. This project also demonstrates that their strong expression in mouse heart inducescardiomyopathy, partially clarifying its molecular mechanisms.

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (23 results)

All 2013 2012 2011 2010 Other

All Journal Article (7 results) (of which Peer Reviewed: 7 results) Presentation (15 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Forced Expression of miR-143 Represses ERK5/c-Myc and p68/p72 Signaling in Concert with miR-145 in Gut Tumors of ApcMin Mice.2012

    • Author(s)
      Takaoka Y., Shimizu Y., Hasegawa H., Ouchi Y., Qiao S., Nagahara M., Ichihara M., Lee J.D., Adachi K., Hamaguchi M., Iwamoto T.
    • Journal Title

      PLoS ONE

      Volume: 7(8)

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Forced Expression of miR-143 Represses ERK5/c-Myc and p68/p72 Signaling in Concert with miR-145 in Gut Tumors of ApcMin Mice.2012

    • Author(s)
      Yuji Takaoka
    • Journal Title

      PLoS ONE

      Volume: 7 Issue: 8 Pages: e42137-e42137

    • DOI

      10.1371/journal.pone.0042137

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Mimosine-Induced Apoptosis in C6 Glioma Cells Requires the Release of Mitochondria-Derived Reactive Oxygen Species and p38, JNK Activation2012

    • Author(s)
      Schanlou Qiao
    • Journal Title

      Neurochemical Research

      Volume: 37 Pages: 417-427

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A novel hairless mouse model for malignant melanoma2012

    • Author(s)
      Nguyen Dinh Thang
    • Journal Title

      Journal of Dermatological Science

      Volume: 65 Pages: 207-212

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] β-Catenin signaling regulates the timing of cell differentiation in mouse retinalprogenitor cells2011

    • Author(s)
      Yasuo Ouchi
    • Journal Title

      Molecular and Cellular Neuroscience

      Volume: 46 Pages: 770-780

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] β-Catenin signaling regulates the timing of cell differentiation in mouse retinal progenitor cells.2011

    • Author(s)
      Yasuo Ouchi
    • Journal Title

      Molecular and Cellular Neuroscience

      Volume: (In press)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Reduced adult hippocampal neurogenesis and working memory deficits in the DGCR8-deficient mouse model of 22q11 deletion-associated schizophrenia can be rescued by Igf2.

    • Author(s)
      Ouchi Y.、 Banno Y., Shimizu Y., Ando S., Hasegawa H., Adachi K., Iwamoto T.
    • Journal Title

      J.Neuroscience

      Volume: (in press)

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Presentation] Deficiency of DGCR8 gene, a potential gene for 22q11 deletion syndrome, decreases adult neurogenesis in the mouse hippocampal dentate gyrus2011

    • Author(s)
      大内靖夫
    • Organizer
      日本分子生物学会年会(BMB2011)
    • Place of Presentation
      パシフィコ横浜(横浜市)
    • Year and Date
      2011-12-13
    • Related Report
      2011 Annual Research Report
  • [Presentation] The heterochronic gene Lin-28 regulates cell proliferation and neural expansion during early development in zebrafish2011

    • Author(s)
      山本純矢
    • Organizer
      日本分子生物学会年会(BMB2011)
    • Place of Presentation
      パシフィコ横浜(横浜市)
    • Year and Date
      2011-12-13
    • Related Report
      2011 Annual Research Report
  • [Presentation] miR-143トランスジェニックマウスは内在性miR-145を誘導しβカテニンシグナルを制御することにより腸管腫瘍を抑制する2011

    • Author(s)
      高岡祐司
    • Organizer
      第70回日本癌学会
    • Place of Presentation
      名古屋国際会議場(名古屋市)
    • Year and Date
      2011-10-05
    • Related Report
      2011 Annual Research Report
  • [Presentation] 22q11欠損症候群モデルマウスにおけるDGCR8遺伝子の欠損は成体マウス海馬歯状回に内在する神経幹細胞の増殖を低下させる2011

    • Author(s)
      大内靖夫
    • Organizer
      第34回日本神経科学会大会
    • Place of Presentation
      パシフィコ横浜(横浜市)
    • Year and Date
      2011-09-16
    • Related Report
      2011 Annual Research Report
  • [Presentation] The heterochrinic gene Lin-28 regulates cell proliferation and neural expansion during early development in zebrafish2011

    • Author(s)
      山本純矢
    • Organizer
      17th Japanese medaka and zebrafish meeting
    • Place of Presentation
      東レ総合研修センター(三島市)
    • Year and Date
      2011-09-08
    • Related Report
      2011 Annual Research Report
  • [Presentation] 癌抑制性マイクロRNAのfeed backネットワーク2011

    • Author(s)
      高岡祐司
    • Organizer
      第3回日本RNAi研究会
    • Place of Presentation
      グランドプリンスホテル広島(広島市)
    • Year and Date
      2011-08-27
    • Related Report
      2011 Annual Research Report
  • [Presentation] DGCR8遺伝子ヘテロ欠損型統合失調症モデルマウス海馬歯状回における神経幹細胞の解析2011

    • Author(s)
      大内靖夫
    • Organizer
      第3回日本RNAi研究会
    • Place of Presentation
      グランドプリンスホテル広島(広島市)
    • Year and Date
      2011-08-26
    • Related Report
      2011 Annual Research Report
  • [Presentation] miR-143トランスジェニックマウスは内在性miR-145を誘導し・カテニンシグナルを制御することにより腸管腫瘍を抑制する2011

    • Author(s)
      高岡祐司、岩本隆司、大内靖夫、濱口道成
    • Organizer
      第70回日本癌学会、名古屋国際会議場
    • Place of Presentation
      名古屋市
    • Related Report
      2012 Final Research Report
  • [Presentation] 癌抑制性マイクロRNAのfeed backネットワーク2011

    • Author(s)
      高岡祐司、清水裕子、大内靖夫、岩本隆司
    • Organizer
      第3回日本RNAi研究会、グランドプリンスホテル広島
    • Place of Presentation
      広島市
    • Related Report
      2012 Final Research Report
  • [Presentation] 腸管上皮細胞におけるRNA結合分子Lin28の機能解析2010

    • Author(s)
      大内靖夫
    • Organizer
      BMB2010
    • Place of Presentation
      神戸国際会議場 他(兵庫県)
    • Year and Date
      2010-12-09
    • Related Report
      2010 Annual Research Report
  • [Presentation] Zebrafishを用いたRNA結合タンパク質Lin28の機能解析、神戸2010

    • Author(s)
      山本純矢
    • Organizer
      BMB2010
    • Place of Presentation
      神戸国際会議場 他(兵庫県)
    • Year and Date
      2010-12-07
    • Related Report
      2010 Annual Research Report
  • [Presentation] 家族性大腸腺腫症モデルマウスにおけるmiR-143の発がん抑制機能解析2010

    • Author(s)
      清水裕子
    • Organizer
      第2回日本RNAi研究会
    • Place of Presentation
      グランドプリンスホテル広島(広島県)
    • Year and Date
      2010-08-28
    • Related Report
      2010 Annual Research Report
  • [Presentation] Altered brain microRNA biogenesis affects neural stem cell proliferation in mouse hippocampal dentate gyrus.2010

    • Author(s)
      大内靖夫
    • Organizer
      第8回幹細胞シンポジウム
    • Place of Presentation
      淡路夢舞台国際会議場(兵庫県)
    • Year and Date
      2010-05-15
    • Related Report
      2010 Annual Research Report
  • [Presentation] crosstalk betweenp53 network and microRNA processingpathway2010

    • Author(s)
      Suzuki H I, Yamagata K, Sugimoto K,Iwamoto T, Kato S, Miyazono K.: Dynamicsof microRNA biogenesis
    • Organizer
      BMB2010
    • Place of Presentation
      神戸ポートアイランド,神戸市
    • Related Report
      2012 Final Research Report
  • [Presentation] 家族性大腸腺腫症モデルマウスにおけるmiR-143 の発がん抑制機能解析2010

    • Author(s)
      Shimizu Y., Hasegawa H., Ouchi Y.,Iwamoto T.
    • Organizer
      第2回日本RNAi研究会、グランドプリンスホテル広島
    • Place of Presentation
      広島市
    • Related Report
      2012 Final Research Report
  • [Patent(Industrial Property Rights)] 神経幹細胞の増殖促進剤及びその用途2013

    • Inventor(s)
      大内 靖夫,岩本 隆司
    • Industrial Property Rights Holder
      中部大学
    • Industrial Property Number
      2013-000289
    • Filing Date
      2013-01-10
    • Related Report
      2012 Final Research Report

URL: 

Published: 2010-08-23   Modified: 2019-07-29  

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