Non-invasive in vivo fluorescence imaging in allogeneic hematopoi etic stem cell transplantation mouse model

• Project/Area Number: 22590372
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Experimental pathology
• Research Institution: National Cancer Center Japan
• Principal Investigator: IKARASHI Yoshinori 独立行政法人国立がん研究センター, 研究所, 主任研究員 (70280782)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 同種造血幹細胞移植 / 移植片対宿主病 / 生体内蛍光イメージング / GFP / 生体蛍光イメージング / GVHD / GFP-Tgマウス / マルチカラー / 蛍光イメージン / GFP-トランスジェニックマウス / 非侵襲 / 皮膚

Research Abstract

In this study, we established non-invasive in vivo fluorescence imaging which enable understanding the process of the graft-versus-host disease and graft-versus-leukemia after allogeneic hematopoietic stem cell transplantation. The localization and movement of donor cells from fluorescent protein transgenic mice were non-invasively visualized in the ear pinna. Furthermore, this imaging is very useful tool for evaluation of immunomoduratory drugs for allogeneic hematopoietic stem cell transplantation.

Research Products

• [Journal Article] Real-time zn vzvo cellular imaging of graft-versus-host disease and its reaction to immunomodulatory reagents, Immunology Letters (2012)
  • Author(s): Takahiro Yamazaki, Yoshinori Ikarashi et al.
  • Journal Title: Immunology Letters Volume: 144巻 Issue: 1-2 Pages: 33-40
  • DOI: 10.1016/j.imlet.2012.03.004
  • Peer Reviewed
• [Journal Article] In vivo delivery of interferon-α gene enhances tumor immunity and suppresses immunotolerance in reconstituted lymphopenic hosts (2011)
  • Author(s): Narumi, K., Udagawa, T., Kondoh, A., Kobayashi, A., Hara, H., Ikarashi, Y., Ohnami, S., Takeshita, F., Ochiya, T., Okada, T., Yamagishi, M., Yoshida, T., Aoki, K.
  • Journal Title: Gene Therapy Volume: 287 Issue: 1 Pages: 1397-1405
  • DOI: 10.1038/gt.2011.73
  • Peer Reviewed
• [Journal Article] Administration route-dependent induction of antitumor immunity by interferon-alpha gene transfer (2010)
  • Author(s): Kenta Narumi
  • Journal Title: Cancer Science Volume: 101 Pages: 1686-1694
  • Peer Reviewed
• [Presentation] NKT cell-ligand inhibits donor engraftment after allogeneic hematopoietic stem cell transplantation (2012)
  • Author(s): Yoshinori Ikarashi
  • Organizer: 日本癌学会
  • Place of Presentation: 札幌市
  • Year and Date: 2012-09-20
• [Presentation] Suppression of regulatory T cells enhances antitumor immunity induced by IFN-alpha gene transfer to pancreatic cancer. (2012)
  • Author(s): Aida K
  • Organizer: 日本癌学会
  • Place of Presentation: 札幌市
• [Presentation] Autologus hematopoietic stem cell transplantation suppresses immunetolerant environment in tumors (2011)
  • Author(s): Takeshi Udagawa
  • Organizer: 日本癌学会
  • Place of Presentation: 名古屋市
  • Year and Date: 2011-10-05
• [Presentation] Inhibition of regulatory T cell enhance antitumor immunity induced by intratumoral IFN-α gene transfer (2011)
  • Author(s): Aida K, Udagawa T, Narumi K, Suzuki K, Goto N, Ikarashi Y, Ohnami S, Yoshida T, Aoki K
  • Organizer: 日本癌学会
  • Place of Presentation: 名古屋市
  • Year and Date: 2011-10-05
• [Presentation] Autologous hematopoietic stem cell transplantation suppresses immunotolerant environment in tumors (2011)
  • Author(s): Udagawa T, Narumi K, Aida K, Suzuki K, Goto N, Ikarashi Y, Ohnami S, Yoshida T, Aoki K
  • Organizer: 日本癌学会
  • Place of Presentation: 名古屋市
  • Year and Date: 2011-10-05
• [Presentation] The effect of NKT cell ligand for donor cell engraftment by In vzvo fluorescence imaging in Allogeneic HSCT (2011)
  • Author(s): Yoshinori Ikarashi
  • Organizer: 日本癌学会
  • Place of Presentation: 名古屋市
  • Year and Date: 2011-10-03
• [Presentation] The effect of NKT cell ligand for donor cell engraftment by in vivo fluo rescence imaging in Allogeneic HSCT (2011)
  • Author(s): Ikarashi Y, Heike Y, Kim SW, Nakagama H, Aoki K
  • Organizer: 日本癌学会
  • Place of Presentation: 名古屋市
  • Year and Date: 2011-10-03
• [Presentation] Screening of immunomodulating drugs for graft-versus-host disease by in vivo fluorescence imaging (2010)
  • Author(s): Yoshinori Ikarashi
  • Organizer: ISCT Asia-Pacific Regional meeting
  • Place of Presentation: 宮崎市
  • Year and Date: 2010-10-18
• [Presentation] Differential chimerism of Langerhans cells, dermal dendritic cells and dendritic epidermal T cells after nonmyeloablative allogeneic hematopoietic stem cell transplantation. (2010)
  • Author(s): Yoshinori Ikarashi
  • Organizer: International Symposium on Dendritic Cells in Fundamental and Clinical Immunology
  • Place of Presentation: Lugano, Switzerland
  • Year and Date: 2010-10-18
• [Presentation] Differential chimerism of Langerhans cells, dermal dendritic cells and dendritic epidermal T cells after nonmyeloablativeallogeneic hematopoietic stem cell transplantation (2010)
  • Author(s): Yoshinori Ikarashi
  • Organizer: International Symposium on Dendritic Cells in Fundamental and Clinical Immunology
  • Place of Presentation: ルガーノスイス
  • Year and Date: 2010-09-29
• [Presentation] Noninvasive monitoring of donor cell infiltration in mouse graft-versus-host disease using in vivo fluorescence imaging (2010)
  • Author(s): Yoshinori Ikarashi
  • Organizer: 日本癌学会
  • Place of Presentation: 大阪市
  • Year and Date: 2010-09-24
• [Remarks] 独立行政法人 国立がん研究センター 研究所
  • URL: http://www.ncc.go.jp/jp/nccri/index.html
• [Remarks]