Development of nuclear DNA markers for phylogenetic classification and population genetic analyses

• Project/Area Number: 22590376
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Parasitology (including Sanitary zoology)
• Research Institution: Asahikawa Medical College
• Principal Investigator: NAKAO Minoru 旭川医科大学, 医学部, 准教授 (70155670)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2010: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 条虫類 / 系統分類 / 核DNAマーカー / 蛋白遺伝子 / 集団遺伝

Research Abstract

Single-locus nuclear DNA markers for phylogenetic classification and population genetic analyses have been developed for cestodes, and universal PCR primers to amplify the marker loci were desigend. A phylogenetic analysis of taeniid cestodes using the markers accelerated the taxonomic revision of the Taeniidae. The family have been devided into the genera Taenia, Hydatigera, Versteria and Echinococcus.

Research Products

• [Journal Article] Molecular phylogeny of the genus Taenia (Cestoda: Taeniidae): Proposals for the resurrection of Hydatigera Lamarck, 1816 and the creation of a new genus Versteria (2013)
  • Author(s): Nakao M, Lavikainen A, Iwaki T, Haukisalmi V, Konyaev S, Oku Y, Okamoto M, Ito A.
  • Journal Title: International Journal for Parasitology Volume: 43(6) Issue: 6 Pages: 427-37
  • DOI: 10.1016/j.ijpara.2012.11.014
  • Peer Reviewed
• [Journal Article] Mitochondrial phylogeny of the genus Echinococcus (Cestoda: Taeniidae) with emphasis on relationships among Echinococcus canadensis genotypes. (2013)
  • Author(s): Nakao M, Yanagida T, Konyaev S, Lavikainen A, Ito A.
  • Journal Title: Parasitology Volume: (in press)
  • Peer Reviewed
• [Journal Article] Phylogeny-based taxonomy of the genus Echinococcus (Cestoda: Taeniidae) (2013)
  • Author(s): Nakao M, Lavikainen A, Yanagida T, Ito A.
  • Journal Title: International Journal for Parasitology Volume: (in press)
  • Peer Reviewed
• [Journal Article] Phylogenetic relationships within Echinococcus and Taenia tapeworms (Cestoda: Taeniidae): an inference from nuclear protein-coding genes (2011)
  • Author(s): Knapp J, Nakao M, Yanagida T, Okamoto M, Saarma U, Lavikainen A, Ito A.
  • Journal Title: Molecular Phylogenetics and Evolution Volume: 61 Issue: 3 Pages: 628-638
  • DOI: 10.1016/j.ympev.2011.07.022
  • Peer Reviewed
• [Presentation] テニア科条虫の分類学的改訂 (2013)
  • Author(s): 中尾 稔
  • Organizer: 第82回日本寄生虫学会大会
  • Place of Presentation: 東京医科歯科大学
• [Presentation] テニア科条虫の分類学的改訂 (2013)
  • Author(s): 中尾 稔
  • Organizer: 第８２回日本寄生虫学会大会
  • Place of Presentation: 東京医科歯科大学
• [Presentation] ミトコンドリアゲノムから推定したテニア科条虫の系統関係 (2012)
  • Author(s): 中尾 稔、伊藤 亮
  • Organizer: 第81回日本寄生虫学会大会
  • Place of Presentation: 兵庫医科大学
• [Presentation] 核DNAマーカーから推定したテニア科条虫の分岐年代 (2011)
  • Author(s): 中尾 稔、Knapp Jenny、柳田哲矢、伊藤 亮
  • Organizer: 第80回日本寄生虫学会大会
  • Place of Presentation: 東京慈恵会医科大学