Functional analysis of motility-related genes of Trypanosoma brucei: relationship with cellular morphology and apoptosis

• Project/Area Number: 22590380
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Parasitology (including Sanitary zoology)
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: TAKASHI Suzuki 東京医科歯科大学, 大学院・医歯学総合研究科, 特任教授 (70305530)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: アポトシス / 鞭毛 / アフリカトリパノソーマ原虫 / トリパノソーマ / 動き / 細胞形態変化

Research Abstract

Double knock-down analysis of TbUNC119 and its binding molecule showed cellular apoptosis, morphological change (extended cell shape), and reduced motility in insect-form trypanosome cells. Among those phenotypes, extended cell shape was firstly observed. This was revealed to be at least in part by up-regulated endocytosis. In bloodstream-form trypanosome cells, TbUNC119 was expressed in flagellum and an area around flagella pocket, only in which endocytosis occurred. Functions of TbUNC119 and its binding protein were thus considered to include regulation of cellular endocytosis.

Research Products

• [Journal Article] Shifting the focus from open release of genetically modified mosquitoes, to controlled individualized treatment for infectious diseases (2012)
  • Author(s): 鈴木高史（Dziedzom K. de Souza）
  • Journal Title: Asia Pacific Journal of Molecular Biology and Biotechnology Volume: 20 Pages: 117-118
  • Peer Reviewed
• [Journal Article] Differential KineticActivities of Glycerol Kinase among African Trypanosome Species: Phylogenetc and Therapeutic Implications (2011)
  • Author(s): Ohashi-Suzuki M, Yabu Y, Ohshima S, Nakamura K, Kido Y, Sakamoto K, Kita K, Ohta N, Suzuki T
  • Journal Title: J Vet Med Sci Volume: 73(5) Pages: 615-21
  • Peer Reviewed
• [Journal Article] Administrative practices of health professionals and use of artesunate-amodiaquine by community members for treating uncomplicated malaria in southern Ghana: implications for artemisinin-based combination therapy deployment (2011)
  • Author(s): Kwansa-Bentum B, Ayi I, Suzuki T,Otchere J, Kumagai T, Anyan WK, Asahi H, Akao N, Wilson MD, Boakye DA, Ohta N
  • Journal Title: Trop Med Int Health Volume: 10. doi:10.1111n
  • Peer Reviewed
• [Journal Article] Plasmodium falciparum isolates from southern Ghana exhibit polymorphisms in the SERCA-type PfATPase6 though sensitive to artesunate in vitro (2011)
  • Author(s): Kwansa-Bentum B, Ayi I, Suzuki T, Otchere J, Kumagai T, Anyan WK, Osei JH, Asahi H, Ofori MF, Akao N, Wilson MD,Boakye DA, Ohta N
  • Journal Title: Malar J Volume: 10:187
  • Peer Reviewed
• [Journal Article] Differential Kinetric Activities of Glycerol Kinase among African Trypanosome Species : Phylogenetic and Therapeutic Implications (2011)
  • Author(s): 鈴木高史(大橋光子)
  • Journal Title: Journal of Veterinary Medical Science Volume: 73 Pages: 615-621
  • Peer Reviewed
• [Journal Article] Plasmodium falciparum isolates from southern Ghana exhibit polymorphisms in the SERCA-type PfATPase6 though sensitive to artesunate in vitro (2011)
  • Author(s): 鈴木高史(Kwansa-Bentum Bethel)
  • Journal Title: Malaria Journal Volume: 11 Issue: 1 Pages: 187-187
  • DOI: 10.1186/1475-2875-10-187
  • Peer Reviewed
• [Journal Article] The biological investigation of prostacyclin in preeclamptic women seen reduced endothelial function (2010)
  • Author(s): Yamamoto T, Suzuki Y, Kojima K, Suzumori N, Suzuki T
  • Journal Title: Hypertens Pregnancy Volume: 29(4) Pages: 484-91
  • Peer Reviewed
• [Journal Article] TbLJNC119 and its binding protein are essential for propagation motitity, and morphogenesis of Trypansoma brucei procylic form celIs. (2010)
  • Author(s): 鈴木高史(大島茂)
  • Journal Title: PloS One Volume: 5
  • Peer Reviewed
• [Journal Article] Purification and kinetic characterization of recombinant alternative oxidase from Trypanosoma brucei brucei (2010)
  • Author(s): 鈴木高史(城戸康年)
  • Journal Title: Biochim Biophys Acta Volume: 1797
  • Peer Reviewed
• [Journal Article] Trypanosome altemative oxidase, a potential therapeutic targct for sleeping sickness, is conserved among Trypanosoma brucei subspecies. (2010)
  • Author(s): 鈴木高史(中村康介)
  • Journal Title: Parasitology International Volume: 59 Pages: 560-564
  • Peer Reviewed
• [Presentation] Finding a Novel Drug Target for African Trypanosomiasis (2013)
  • Author(s): Suzuki T
  • Organizer: Asian-AfricanResearch Forum on Emerging andReemerging Infections
  • Place of Presentation: Tokyo (Japan)
  • Year and Date: 2013-01-23
• [Presentation] Molecular cloning and functional analysis of Trypanosoma brucei motility-related genes (2013)
  • Author(s): Suzuki T, Ohshima S, Ohashi-Suzuki M, Dadzie S, Boakye D, Ohta N
  • Organizer: Asian African Research Forum for on Emerging and Reemerging Infections
  • Place of Presentation: Kobe (Japan)
  • Year and Date: 2013-01-11
• [Presentation] Plasmodium falciparum isolates from southern Ghana exhibit polymorphisms in the SERCA-type PfATPase6 though sensitive to artesunate in vitro (2013)
  • Author(s): Kwansa-Bentum B, Ayi I, Suzuki T, Otchere J, Kumagai T, Anyan WK, Osei JH, Asahi H, Ofori MF, Akao N, Wilson MD, Boakye DA, Ohta N
  • Organizer: Asian African Research Forum for on Emerging and Reemerging Infections
  • Place of Presentation: Kobe (Japan)
  • Year and Date: 2013-01-11
• [Presentation] Prevalence of Placental Toxoplasmosis in the Greater Accra Region of Ghana (2013)
  • Author(s): 鈴木高史（Kofi Dadzie Kwofie）
  • Organizer: International Society for Travel Medicine (ISTM) scientific conference
  • Place of Presentation: Maastricht Exposition Conference Centre (MECC), Maastricht, Netherlands
• [Presentation] Finding a Novel Drug Target for African Trypanosomiasis (2013)
  • Author(s): 鈴木高史
  • Organizer: Asian-African Research Forum on Emerging and Reemerging Infections 2013
  • Place of Presentation: Akio Suzuki Memorial Hall, Tokyo Medical and Dental University
  • Invited
• [Presentation] Epidemiology of Toxoplasmosis in Ghana (2013)
  • Author(s): 鈴木高史（Irene Ayi）
  • Organizer: Asian-African Research Forum on Emerging and Reemerging Infections 2013
  • Place of Presentation: Akio Suzuki Memorial Hall, Tokyo Medical and Dental University
• [Presentation] Bio-efficacy, user perception and acceptability of some selected pyrethoid-based mosquito coils in controlling An. gambiae s.l., a malaria vector in some parts of the Greater Accra region of Ghana (2012)
  • Author(s): Dadzie S, Adu-Acheampong S, Kyerematen R, Williams J, Suzuki T, Ohta N, Appawu M, Boakye DA
  • Organizer: Asian African Research Forum for on Emerging and Reemerging Infections
  • Place of Presentation: Kobe (Japan)
  • Year and Date: 2012-01-11
• [Presentation] Molecular cloning and functional analysis of Trypanosoma brucei motility-related gene (2012)
  • Author(s): 鈴木高史
  • Organizer: Asia Africa Research Forum
  • Place of Presentation: 国際会議場(神戸)
  • Year and Date: 2012-01-11
• [Presentation] Plasmodium falciparum isolates from southern Ghana exhibit polymorphisms in the SERCA-type PfATPase6 though sensitive to artesunate in vitro (2012)
  • Author(s): 鈴木高史(Kwansa-Bentum Bethel)
  • Organizer: Asia Africa Research Forum
  • Place of Presentation: 国際会議場(神戸)
  • Year and Date: 2012-01-11
• [Presentation] Indication of High Risk of Mother-to-Child transmission of Toxoplasma gondii infection in the Greater Accra Region of Ghana (2012)
  • Author(s): 鈴木高史（Kofi Dadzie Kwofie）
  • Organizer: 6th Annual College of Health Sciences conference
  • Place of Presentation: College of Physicians and Surgeons, Accra
• [Presentation] Indication of High Risk of Mother-to-Child transmission of Toxoplasma gondii infection in the Greater Accra Region of Ghana (2012)
  • Author(s): 鈴木高史（Kofi Dadzie Kwofie）
  • Organizer: American Society for Tropical Medicine and Hygiene (ASTMH) scientific conference
  • Place of Presentation: Marriot Marquis, Atlanta GA, USA
• [Presentation] Molecular biological and epidemiological study of African trypanosomiasis (2011)
  • Author(s): 鈴木高史
  • Organizer: SATREPS Joint Workshop
  • Place of Presentation: ガーナ大学(アクラ)
  • Year and Date: 2011-12-12
• [Presentation] Detection of Trypanosoma species from Tsetse flies anddomestic animals in the Central and Volta regions of Ghana (2011)
  • Author(s): Alhassan A, Nakayima J, Nakao R, Suzuki T, Sugimoto C
  • Organizer: SATREPS Joint Workshop
  • Place of Presentation: Accra (Ghana)
  • Year and Date: 2011-12-04
• [Presentation] Plasmodium falciparum isolates from southern Ghana exhibit polymorphisms in the SERCA-type PfATPase6 though sensitive to artesunate in vitro (2011)
  • Author(s): Kwansa-Bentum B, Ayi I, Suzuki T, Otchere J, Kumagai T, Anyan WK, Osei JH, Asahi H, Ofori MF, Akao N, Wilson MD, Boakye DA, Ohta N
  • Organizer: SATREPS Joint Workshop
  • Place of Presentation: Accra (Ghana)
  • Year and Date: 2011-12-04
• [Presentation] Molecular biological and epidemiological study of African trypanosomiasis (2011)
  • Author(s): Suzuki T, Ohshima S, Boakye D, Alhassan A, Bosompem K, Ohta N
  • Organizer: SATREPS Joint Workshop
  • Place of Presentation: Accra (Ghana)
  • Year and Date: 2011-12-03
• [Presentation] Molecular cloning and functional analysis of Trypanosoma brucei motility-related genes. (2010)
  • Author(s): 大橋光子(鈴木高史)
  • Organizer: 12^<th> Internation Congress of Parasitology
  • Place of Presentation: 国際展示場(メルボルン)
  • Year and Date: 2010-08-18